Study of Gene Expression During in Vitro Culture of Tobacco thin Cell Layers by two-Dimensional Electrophoresis of Proteins

Plant Aging ◽  
1990 ◽  
pp. 207-213 ◽  
Author(s):  
Kiêm Tran Thanh Van ◽  
Michel Zivy ◽  
Alain Cousson ◽  
Hervé Thiellement
1986 ◽  
Vol 64 (9) ◽  
pp. 2073-2081 ◽  
Author(s):  
Robert S. Jeng

Soluble mycelial proteins from Ophiostoma ulmi (Buism.) Nannf., the causal agent of Dutch elm disease, were separated by analytical electrofocusing and two-dimensional electrophoresis in polyacrylamide gels. Results showed the aggressive and nonaggressive strains of this pathogen each had about 60 Coomassie blue stained bands having isoelectric points from 3 to 7. Both strains of this fungus had their own characteristic electrofocusing patterns. Nonaggressive isolate S116, for example, lacked two protein bands, one near the anode and one near the cathode, but it had five additional protein bands distributed from pH 4 to 6. Two-dimensional electrophoresis of total soluble proteins depicted that there were 36 proteins found to be specific for the nonaggressive isolate S116 and 12 proteins for the aggressive isolate RR2.


The Analyst ◽  
1994 ◽  
Vol 119 (6) ◽  
pp. 1341-1344 ◽  
Author(s):  
S. M. Saqlan Naqvi ◽  
V. Cengiz Özalp ◽  
H. Avni Öktem ◽  
Meral Yücel

2006 ◽  
Vol 114 (2) ◽  
pp. 147-153 ◽  
Author(s):  
Rui Vitorino ◽  
Sofia de Morais Guedes ◽  
Rita Ferreira ◽  
Maria Joao C. Lobo ◽  
Jose Duarte ◽  
...  

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