Myosin Head Movements during Isometric Contraction Studied by X-Ray Diffraction of Single Frog Muscle Fibres

1998 ◽  
pp. 265-270 ◽  
Author(s):  
Massimo Reconditi ◽  
Ian Dobbie ◽  
Malcolm Irving ◽  
Olivier Diat ◽  
Peter Boesecke ◽  
...  
Keyword(s):  
Isometric Contraction ◽  
Myosin Head ◽  
Frog Muscle ◽  
Head Movements ◽  
Muscle Fibres ◽  
X Ray Diffraction ◽  
X Ray

scholarly journals A combined mechanical and X‐ray diffraction study of stretch potentiation in single frog muscle fibres

2000 ◽  
Vol 526 (3) ◽  
pp. 589-596 ◽  
Author(s):  
M. Linari ◽  
L. Lucii ◽  
M. Reconditi ◽  
M. E. Vannicelli Casoni ◽  
H. Amenitsch ◽  
...  
Keyword(s):  
Diffraction Study ◽  
Frog Muscle ◽  
Muscle Fibres ◽  
X Ray Diffraction ◽  
X Ray

scholarly journals Structural responses to the photolytic release of ATP in frog muscle fibres, observed by time-resolved X-ray diffraction

1999 ◽  
Vol 520 (3) ◽  
pp. 681-696 ◽  
Author(s):  
Andrey K. Tsaturyan ◽  
Sergey Y. Bershitsky ◽  
Ronald Burns ◽  
Zhen-He He ◽  
Michael A. Ferenczi
Keyword(s):  
Frog Muscle ◽  
Muscle Fibres ◽  
X Ray Diffraction ◽  
Time Resolved ◽  
X Ray ◽  
Structural Responses

scholarly journals X-Ray Diffraction Study of the Effect of Temperature on the Structure of Mammalian Muscle at Rest and During Isometric Contraction

2011 ◽  
Vol 100 (3) ◽  
pp. 312a
Author(s):  
Marco Caremani ◽  
Massimo Reconditi ◽  
Elisabetta Brunello ◽  
Marco Linari ◽  
David Gore ◽  
...  
Keyword(s):  
Diffraction Study ◽  
Isometric Contraction ◽  
Effect Of Temperature ◽  
X Ray Diffraction ◽  
X Ray ◽  
Mammalian Muscle

scholarly journals X-ray diffraction studies of the contractile mechanism in single muscle fibres

2004 ◽  
Vol 359 (1452) ◽  
pp. 1883-1893 ◽  
Author(s):  
K. C. Holmes ◽  
D. R. Trentham ◽  
R. Simmons ◽  
Vincenzo Lombardi ◽  
Gabriella Piazzesi ◽  
...  
Keyword(s):  
Fibre Length ◽  
Elastic Response ◽  
Muscle Fibres ◽  
X Ray Diffraction ◽  
X Ray ◽  
Rapid Changes ◽  
Low Load ◽  
Intact Muscle ◽  
Motor Actions

The molecular mechanism of muscle contraction was investigated in intact muscle fibres by X–ray diffraction. Changes in the intensities of the axial X–ray reflections produced by imposing rapid changes in fibre length establish the average conformation of the myosin heads during active isometric contraction, and show that the heads tilt during the elastic response to a change in fibre length and during the elementary force generating process: the working stroke. X–ray interference between the two arrays of myosin heads in each filament allows the axial motions of the heads following a sudden drop in force from the isometric level to be measured in situ with unprecedented precision. At low load, the average working stroke is 12 nm, which is consistent with crystallographic studies. The working stroke is smaller and slower at a higher load. The compliance of the actin and myosin filaments was also determined from the change in the axial spacings of the X–ray reflections following a force step, and shown to be responsible for most of the sarcomere compliance. The mechanical properties of the sarcomere depend on both the motor actions of the myosin heads and the compliance of the myosin and actin filaments.

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