Histology of Muscle Development in Pigs, Epigenetics from Myotubes to Tapered Fibres

Pre-natal muscle development in pigs starts with myotubes (axial nuclei in a tube of myofibrils) and secondary fibres (peripheral nuclei on an axial strand of myofibrils). By the time of birth, the nuclei of myotubes move to a peripheral position like secondary fibres. As pre-natal secondary fibres grow in length, the number of fibres in a transverse section may appear to increase. This stereology may also occur in post-natal muscles that have tapered fibres anchored in endomysial connective tissue around adjacent fibres and with one or both ends not reaching the end of their fasciculus. Up to 100 days gestation, Peroneus longus (no tapered fibres) had larger (P < 0.001) diameter secondary fibres than Longissimus thoracis (with tapered fibres). Up to 100 days gestation, no radial growth of secondary fibres was detected, but myotubes decreased in diameter (P < 0.001). From a curve showing the relative numbers of myotubes and secondary fibres, it was deduced that approximately 80% of muscle fibres in pigs are derived from secondary fibres. In post-natal Sartorius muscle there was an increase (P < 0.005) in the apparent number of muscle fibres attributed to longitudinal growth of tapered fibres. Myotubes located centrally within their fasciculi had the same position as slow-contracting fibres with a high myoglobin content in adult muscle. Post-natal changes in muscle fibre histochemistry were achieved through transitional types, probably neurally regulated rather than by differential longitudinal growth of tapered endings. Secondary fibres are important – they give rise to both the majority of muscle fibres in adult pigs and affect subsurface optical pathways and pork colourimetry.

Prenatally Diagnosed Infantile Myofibroma of Sartorius Muscle—A Differential for Soft Tissue Masses in Early Infancy

Background: Infantile myofibromatosis (IM) is a soft tissue disease with solitary or multiple benign tumors, and an etiology still unknown. IM is a mesenchymal disorder of early infancy and is more frequent in males. IM may present as a solitary lesion of the skin, bone, muscle, subcutaneous tissue, located at the head, neck, and trunk, with good prognosis; or, as a multicentric form, with or without visceral involvement (heart, lung, gastrointestinal tract, kidney), with a poor prognosis. The definitive diagnosis of IM is confirmed by pathology. Treatment may be conservative, surgical, or chemotherapeutical. Case presentation: A two months old female patient, prenatally diagnosed at 30 weeks, presenting with a tumor on the antero-internal aspect of the left thigh. She was admitted due to rapid postnatal evolution, and the patient required surgery for tumor resection. Previously, clinically, biological and imaging investigations were performed, but the final diagnosis was histological and by immunostaining. The patient had a favorable postoperative outcome. Conclusions: Despite its low frequency, IM should be considered in the differential diagnosis of soft tissue masses at an early age. The clinical form (solitary or multicentric), location, and visceral involvement will dictate the treatment and prognosis.

Exploring bio-parametric solution-based design process for an ephemeral pavilion

This research proposes an experimental design approach to design an ephemeral pavilion located at the Serpentine Gallery in London. The Serpentine Pavilions Programme functions as an experimentation laboratory and, at the same time, public and event spaces, enabling architects to expose their projects and work methodologies. Thus, the methods of Biomimicry and Parametric Design were combined to develop the pavilion. While the first one was used to create an ephemeral pavilion based on the Sartorius muscle, the second was responsible for generating the parametric model from a fast and intuitive manipulation code capable of exploring shape variations. This work explores the solution-based method approached by Badarnah (2012) based on a predefined problem (the pavilion project) and only after that seek some natural inspiration. Firstly, it was investigated the anatomy of the Sartorius muscle. Subsequently, with the domain of the solution, the parametric insertion of the shape was computationally performed. The anatomical study of the sartorius muscle revealed functions such as flexion, abduction, lateral rotation of the thigh, and medial rotation of the knee. Thus, the architectural choices reflect both its narrow and elongated morphology of the muscle and flexibility and rotation aspects. The pavilion also considered the previous Serpentine Pavilions regarding attributes such as area, height, and materials, which with other parameters may be changed using the code implemented in Grasshopper.

Anterior prompt for debridement and myoplasty femoral neck and head osteomyelitis

The known anterior prompts to the femoral neck are non-traumatic, but they exclude the possibility of myoplasty and are not large enough for intra-articular sequestrectomy.Objective. The aim of the study was to develop an anterior prompt to the hip joint for sequestrectomy with myoplasty.Materials and research methods. For surgery neck and head of the hip osteomyelitis we perform a semi-oval incision in the projection of the femoral head, from the anterior spines of the ilium we cut off the tendons of the sartorius and rectus head of the quadriceps femoris muscles. An operating field is formed, which allows sequestrectomy to be performed. Plasty of the osteomyelitis cavity is performed with the proximal parts of the two anterior ileofemoral muscles. The operating field, if necessary, can be increased by temporary transection of the lumbar-iliac muscle tendon.Research results. The key to the anterior surgical prompt to the hip joint is the sartorius and rectus head of the quadriceps. The length of the surgical wound reaches 17–22 cm, and the width is 16–18 cm. With the mobilized proximal part of the sartorius muscle, we fill the entire bone cavity in the neck and head of the femur. Of the 12 operated patients, 11 (91.7 ± 7.6 %) had a stable long-term remission.Conclusion. The proposed approach is less traumatic and allows you to operate at an angle of about 90°. It complements the advantages of the known anterior prompts and is devoid of their disadvantages.

Measurement of Tissue Oximetry in Standing Unsedated and Sedated Horses

Near infrared spectroscopy (NIRS) noninvasively measures peripheral tissue oxygen saturation (StO2) and may be useful to detect early changes in StO2 in anaesthetized and critically ill horses. This study aimed to identify the muscle belly that provided the highest percentage of successful StO2 readings and the highest mean StO2 value. Fifty adult horses were enrolled in a prospective controlled study. StO2 was measured at six different muscles in each horse, for each intervention: hair overlying the muscle was clipped (post clipping: PC), clipped skin was cleaned with chlorhexidine (post-surgical prepping: PP) and medetomidine was administered intravenously (post medetomidine: PM). Mean StO2 values were calculated for each muscle, and a linear effects model was used to assess the effect of muscle group and intervention on StO2. The sartorius muscle gave the highest percentage of successful StO2 values (p < 0.001) and the highest mean (90% CI) StO2 values for the PC, PP and PM interventions. Surgical prepping of the skin increased the success for measurement of StO2 values. For all muscles, administration of medetomidine was associated with lower StO2 values (p < 0.001). In conclusion, of the muscles examined, the sartorius muscle may be the preferred muscle to measure StO2 in horses, and clipping and cleaning of the probe placement site is recommended.

1246 Partial Phalloplasty After Oncologic Resection: A Challenge for Health Centers in Remote Area of Indonesia

Background Restoring penile function for urination and sexual activity, and achieving cosmetic result, are the goals of penile reconstruction including in post-malignancy reconstruction. Lack of studies has reported the usage of radial forearm flap on partial phalloplasty. Case Presentation A 31-years old male presented with fragile, cauliflower-like mass sizing 6x8 cm. The tumor had covered whole glans until the middle shaft of the penis. Plan of treatment for this patient started with complete excision of the tumor (partial penectomy), continued with phalloplasty. Phalloplasty consisted of using radial forearm flap (RFF) for penile foreskin (including glans), reconstructing corporal bodies using sartorius muscle, reshaping the penile glans, and urethroplasty to correct the hypospadic-looking urethra. Radial forearm flap of the non-dominant arm was implanted on the remaining foreskin of the penis, continued with wound care. On the 7th day postoperatively, no signs of necrotic, wound dehiscence, nor pus were observed, with capillary refill time on the distal fingers shown less than 2 seconds. Further evaluation is scheduled. Conclusions Loss of physiologic function usually follows partial penilectomy, which could lead to decrease in psychological quality of life. The main goal of the reconstruction of penile foreskin (including glans) using RFF is to form a newly formed penile glans that would have a cosmetic appearance nearly similar to a normal glans, which could improve its physiologic function and benefit psychologically. A challenge for health care centers in remote is is how to monitor and maintain a viable flap after the reconstruction and to keep a regular evaluation from the patient.