Gene Transfer in Mammalian Cells Using Liposomes as Carriers

1994 ◽  
pp. 118-142 ◽  
Author(s):  
Arun Singhal ◽  
Leaf Huang
Keyword(s):  
Gene Transfer ◽  
Mammalian Cells

Phage Particle-Mediated Gene Transfer to Cultured Mammalian Cells

1982 ◽  
Vol 2 (6) ◽  
pp. 607-616
Author(s):  
Masahiro Ishiura ◽  
Susumu Hirose ◽  
Tsuyoshi Uchida ◽  
Yoshio Hamada ◽  
Yoshiaki Suzuki ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Gene Transfer ◽  
Mammalian Cells ◽  
Phage Particle ◽  
High Efficiency ◽  
Linear Increase ◽  
Recombinant Phage ◽  
Optimal Values ◽  
Simplex Virus

Recombinant phage particles carrying the thymidine kinase (TK) gene of herpes simplex virus type 1, coprecipitated with calcium phosphate, efficiently transformed mouse Ltk − cells to the TK + phenotype. The conditions necessary to achieve high efficiency of transfer of the TK gene by phage particle-mediated gene transfer were investigated. Of the parameters examined, the pH of the buffer used for coprecipitation of phage particles with calcium phosphate, the length of time of coprecipitation, and the length of the adsorption period were found to alter the transfer efficiency significantly. The optimal pH was 6.87 at 25°C. The other optimal values for these parameters were as follows: coprecipitation time, 7 to 20 min; adsorption time, 18 to 30 h. Treatment with dimethyl sulfoxide, glycerol, or sucrose did not enhance gene transfer. The optimal conditions yielded about 1 transformant per 10 5 phage particles per 10 6 cells without carrier DNA. An increase in the dosage of phage particles, up to at least 5 × 10 7 phage particles per 100-mm dish, resulted in a linear increase in the number of transformants. Addition of carrier phage, up to 10 10 phage particles per dish, did not significantly affect the number of transformants.

Surfactant-Mediated Gene Transfer for Mammalian Cells

1997 ◽  
pp. 381-385
Author(s):  
Masamichi Kamihira ◽  
Jun You ◽  
Shinji Iijima
Keyword(s):  
Gene Transfer ◽  
Mammalian Cells

scholarly journals Electric pulse-mediated gene transfer in mammalian cells grown in suspension culture.

1986 ◽  
Vol 11 (2) ◽  
pp. 191-197 ◽  
Author(s):  
Hiroko Hama-Inaba ◽  
Tadahiro Shiomi ◽  
Koki Sato ◽  
Akemi Ito ◽  
Michiki Kasai
Keyword(s):  
Gene Transfer ◽  
Suspension Culture ◽  
Mammalian Cells ◽  
Electric Pulse

Baculovirus-mediated gene transfer into mammalian cells

1998 ◽  
Vol 41 (5) ◽  
pp. 473-478 ◽  
Author(s):  
Ying Zhu ◽  
Yipeng Qi ◽  
Ziye Liu ◽  
Gengfu Xiao
Keyword(s):  
Gene Transfer ◽  
Mammalian Cells
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