Paradoxes of in Situ Polyacrylamide Gel Assays for DNA Polymerase Priming

1984 ◽  
pp. 287-293
Author(s):  
Geoffrey R. Banks
Keyword(s):  
Dna Polymerase ◽  
Polyacrylamide Gel

Human DNA polymerase δ gene maps to region 19q13.3–q13.4 by in Situ hybridization

Genomics ◽  
1992 ◽  
Vol 14 (1) ◽  
pp. 205-206 ◽  
Author(s):  
Robert R. Kemper ◽  
Eugene R. Ahn ◽  
Peng Zhang ◽  
Marietta Y.W.T. Lee ◽  
Mark Rabin
Keyword(s):  
In Situ Hybridization ◽  
Dna Polymerase ◽  
Dna Polymerase Δ ◽  
Human Dna ◽  
Gene Maps

scholarly journals Isolation of mouse myocardial gap junctions.

1980 ◽  
Vol 86 (3) ◽  
pp. 755-764 ◽  
Author(s):  
R W Kensler ◽  
D A Goodenough
Keyword(s):  
Gap Junctions ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
New Method ◽  
Diffuse Band ◽  
Intercalated Disk

A new method is presented for the isolation of an enriched fraction of mouse myocardial gap junctions without the use of exogenous proteases. The junctions appear well preserved morphologically and similar to their appearance in situ. Contaminants of the preparation include fragments of the fascia adherens region of the intercalated disk. SDS polyacrylamide gel electrophoresis of the preparation reveals seven major bands with apparent mol wt of 28,000; 31,000; 33,500; 43,000; 47,000; 49,000; and 57,000. Only the bands at 38,000; 31,000; 33,500; and possibly the diffuse band at 47,000 copurify with the morphologically assayed gap junctions. Evidence is presented that the peptides at 43,000 and 57,000 are contained within the contaminating fascia adherens.

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