Structural Analysis of the Rhodobacter capsulatus bchC Bacteriochlorophyll Biosynthesis Gene

1990 ◽  
pp. 57-64
Author(s):  
Cheryl L. Wellington ◽  
J. Thomas Beatty
Keyword(s):  
Structural Analysis ◽  
Rhodobacter Capsulatus ◽  
Biosynthesis Gene ◽  
Bacteriochlorophyll Biosynthesis

A genetic-physical map of the Rhodobacter capsulatus carotenoid biosynthesis gene cluster

1988 ◽  
Vol 213 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Giovanni Giuliano ◽  
Daniel Pollock ◽  
Henry Stapp ◽  
Pablo A. Scolnik
Keyword(s):  
Gene Cluster ◽  
Rhodobacter Capsulatus ◽  
Physical Map ◽  
Carotenoid Biosynthesis ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

scholarly journals Mutation of an Arginine Biosynthesis Gene Causes Reduced Pathogenicity in Fusarium oxysporum f. sp. melonis

2001 ◽  
Vol 14 (4) ◽  
pp. 580-584 ◽  
Author(s):  
Fumio Namiki ◽  
Michiko Matsunaga ◽  
Mitsuru Okuda ◽  
Iori Inoue ◽  
Kazufumi Nishi ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Fusarium Oxysporum ◽  
Restriction Enzyme ◽  
Argininosuccinate Lyase ◽  
Arginine Biosynthesis ◽  
Biosynthesis Gene ◽  
Remi Mutagenesis ◽  
Auxotrophic Mutation ◽  
Arginine Auxotroph

Restriction enzyme-mediated integration (REMI) mutagenesis was used to tag genes required for pathogenicity of Fusarium oxysporum f. sp. melonis. Of the 1,129 REMI transformants tested, 13 showed reduced pathogenicity on susceptible melon cultivars. One of the mutants, FMMP95–1, was an arginine auxotroph. Structural analysis of the tagged site in FMMP95-1 identified a gene, designated ARG1, which possibly encodes argininosuccinate lyase, catalyzing the last step for arginine biosynthesis. Complementation of FMMP95–1 with the ARG1 gene caused a recovery in pathogenicity, indicating that arginine auxotrophic mutation causes reduced pathogenicity in this pathogen.

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