A genetic-physical map of the Rhodobacter capsulatus carotenoid biosynthesis gene cluster

1988 ◽  
Vol 213 (1) ◽  
pp. 78-83 ◽  
Author(s):  
Giovanni Giuliano ◽  
Daniel Pollock ◽  
Henry Stapp ◽  
Pablo A. Scolnik
Keyword(s):  
Gene Cluster ◽  
Rhodobacter Capsulatus ◽  
Physical Map ◽  
Carotenoid Biosynthesis ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

Organization of the Rhodobacter Capsulatus Carotenoid Biosynthesis Gene Cluster

1991 ◽  
pp. 89-100
Author(s):  
Gregory A. Armstrong ◽  
Marie Albetti ◽  
Francesca Leach ◽  
John E. Hearst
Keyword(s):  
Gene Cluster ◽  
Rhodobacter Capsulatus ◽  
Carotenoid Biosynthesis ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

scholarly journals Isolation and Characterization of Canthaxanthin Biosynthesis Genes from the Photosynthetic Bacterium Bradyrhizobiumsp. Strain ORS278

2000 ◽  
Vol 182 (13) ◽  
pp. 3850-3853 ◽  
Author(s):  
Laure Hannibal ◽  
Jean Lorquin ◽  
Nicolas Angles D'Ortoli ◽  
Nelly Garcia ◽  
Clemence Chaintreuil ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Photosynthetic Bacterium ◽  
Carotenoid Biosynthesis ◽  
Open Reading Frame ◽  
Biosynthesis Gene Cluster ◽  
Reading Frame ◽  
Functional Assignment ◽  
Isolation And Characterization ◽  
Biosynthesis Gene

ABSTRACT A carotenoid biosynthesis gene cluster involved in canthaxanthin production was isolated from the photosyntheticBradyrhizobium sp. strain ORS278. This cluster includes five genes identified as crtE, crtY,crtI, crtB, and crtW that are organized in at least two operons. The functional assignment of each open reading frame was confirmed by complementation studies.

scholarly journals Light-Induced Carotenogenesis in Streptomyces coelicolor A3(2): Identification of an Extracytoplasmic Function Sigma Factor That Directs Photodependent Transcription of the Carotenoid Biosynthesis Gene Cluster

2005 ◽  
Vol 187 (5) ◽  
pp. 1825-1832 ◽  
Author(s):  
Hideaki Takano ◽  
Saemi Obitsu ◽  
Teruhiko Beppu ◽  
Kenji Ueda
Keyword(s):  
Gene Expression ◽  
Gene Cluster ◽  
Sigma Factor ◽  
Streptomyces Coelicolor ◽  
Regulatory Region ◽  
Carotenoid Biosynthesis ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene ◽  
Extracytoplasmic Function Sigma Factor

ABSTRACT Carotenoids are produced by a variety of organisms, but the mechanisms that regulate gene expression leading to carotenoid biosynthesis have been characterized for only a few organisms. In this study, we found that Streptomyces coelicolor A3(2), a gram-positive filamentous bacterium, produces carotenoids under blue light induction. The carotenoid fraction isolated from the cell extract contained multiple compounds, including isorenieratene and β-carotene. The carotenoid biosynthesis gene cluster of S. coelicolor consists of two convergent operons, crtEIBV and crtYTU, as previously shown for Streptomyces griseus. The crtEIBV null mutant completely lost its ability to produce carotenoids. The crt gene cluster is flanked by a regulatory region that consists of two divergent operons, litRQ and litSAB. The lit (light-induced transcription) genes encode a MerR-type transcriptional regulator (LitR), a possible oxidoreductase (LitQ), an extracytoplasmic function sigma factor (σLitS), a putative lipoprotein (LitA), and a putative anti-sigma factor (LitB). S1 protection assay revealed that the promoters preceding crtE (PcrtE), crtY (PcrtY), litR (PlitR), and litS (PlitS) are activated upon illumination. A litS mutant lost both the ability to produce carotenoids and the activities of PcrtE, PcrtY, and PlitS, which suggested that σLitS directs light-induced transcription from these promoters. An RNA polymerase holocomplex containing purified σLitS recombinant protein generated specific PcrtE and PcrtY transcripts in an in vitro runoff transcriptional assay. A litR mutant that had an insertion of the kanamycin resistance gene was defective both in the ability to produce carotenoids and in all of the light-dependent promoter activities. Overexpression of litS resulted in constitutive carotenoid production in both the wild type and the litR mutant. These results indicate that σLitS acts as a light-induced sigma factor that directs transcription of the crt biosynthesis gene cluster, whose activity is controlled by an unknown LitR function. This is the first report to describe light-inducible gene expression in Streptomyces.

A carotenoid biosynthesis gene cluster in Fusarium fujikuroi: the genes carB and carRA

2002 ◽  
Vol 267 (5) ◽  
pp. 593-602 ◽  
Author(s):  
P. Linnemannstöns ◽  
M. Prado ◽  
R. Fernández-Martín ◽  
B. Tudzynski ◽  
J. Avalos
Keyword(s):  
Gene Cluster ◽  
Carotenoid Biosynthesis ◽  
Fusarium Fujikuroi ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

scholarly journals Elucidation of a Carotenoid Biosynthesis Gene Cluster Encoding a Novel Enzyme, 2,2′-β-Hydroxylase, from Brevundimonas sp. Strain SD212 and Combinatorial Biosynthesis of New or Rare Xanthophylls

2005 ◽  
Vol 71 (8) ◽  
pp. 4286-4296 ◽  
Author(s):  
Yasuhiro Nishida ◽  
Kyoko Adachi ◽  
Hiroaki Kasai ◽  
Yoshikazu Shizuri ◽  
Kazutoshi Shindo ◽  
...  
Keyword(s):  
Gene Cluster ◽  
High Performance ◽  
Atmospheric Pressure Chemical Ionization ◽  
Carotenoid Biosynthesis ◽  
Inhibitory Effect ◽  
Combinatorial Biosynthesis ◽  
Array Detector ◽  
Ionization Mass ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

ABSTRACT A carotenoid biosynthesis gene cluster mediating the production of 2-hydroxyastaxanthin was isolated from the marine bacterium Brevundimonas sp. strain SD212 by using a common crtI sequence as the probe DNA. A sequence analysis revealed this cluster to contain 12 open reading frames (ORFs), including the 7 known genes, crtW, crtY, crtI, crtB, crtE, idi, and crtZ. The individual ORFs were functionally analyzed by complementation studies using Escherichia coli that accumulated various carotenoid precursors due to the presence of other bacterial crt genes. In addition to functionally identifying the known crt genes, we found that one (ORF11, named crtG) coded for a novel enzyme, carotenoid 2,2′-β-hydroxylase, which showed intriguingly partial homology with animal sterol-C5-desaturase. When this crtG gene was introduced into E. coli accumulating zeaxanthin and canthaxanthin, the resulting transformants produced their 2-hydroxylated and 2,2′-dihydroxylated products which were structurally novel or rare xanthophylls, as determined by their nuclear magnetic resonance and high-performance liquid chromatography/photodiode array detector/atmospheric pressure chemical ionization mass spectrometry spectral data. The new carotenoid produced was suggested to have a strong inhibitory effect on lipid peroxidation.

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