Analysis of Individual Protein Turnover in Live Animals on a Proteome-Wide Scale

2014 ◽  
pp. 147-154 ◽  
Author(s):  
Stefan Reckow ◽  
Christian Webhofer
Keyword(s):  
Protein Turnover ◽  
Individual Protein ◽  
Wide Scale

scholarly journals Proteome Turnover in the Spotlight: Approaches, Applications & Perspectives

2020 ◽  
pp. mcp.R120.002190
Author(s):  
Alison B. Ross ◽  
Julian David Langer ◽  
Marko Jovanovic
Keyword(s):  
Steady State ◽  
Protein Turnover ◽  
Dynamic Responses ◽  
Protein Homeostasis ◽  
Biological Investigation ◽  
Kinetics And Dynamics ◽  
Wide Scale ◽  
Metabolic Tracing ◽  
External Stimuli ◽  
Synthesis And Degradation

In all cells, proteins are continuously synthesized and degraded in order to maintain protein homeostasis and modify gene expression levels in response to stimuli. Collectively, the processes of protein synthesis and degradation are referred to as protein turnover. At steady state, protein turnover is constant to maintain protein homeostasis, but in dynamic responses, proteins change their rates of synthesis and degradation in order to adjust their proteomes to internal or external stimuli. Thus, probing the kinetics and dynamics of protein turnover lends insight into how cells regulate essential processes such as growth, differentiation, and stress response. Here we outline historical and current approaches to measuring the kinetics of protein turnover on a proteome-wide scale in both steady-state and dynamic systems, with an emphasis on metabolic tracing using stable-isotope-labeled amino acids. We highlight important considerations for designing proteome turnover experiments, key biological findings regarding the conserved principles of proteome turnover regulation, and future perspectives for both technological and biological investigation.

Proteome dynamics in complex organisms: Using stable isotopes to monitor individual protein turnover rates

PROTEOMICS ◽  
2005 ◽  
Vol 5 (2) ◽  
pp. 522-533 ◽  
Author(s):  
Mary K. Doherty ◽  
Colin Whitehead ◽  
Heather McCormack ◽  
Simon J. Gaskell ◽  
Robert J. Beynon
Keyword(s):  
Stable Isotopes ◽  
Protein Turnover ◽  
Turnover Rates ◽  
Individual Protein

Respiratory energy requirements and rate of protein turnover in vivo determined by the use of an inhibitor of protein synthesis and a probe to assess its effect

1994 ◽  
Vol 92 (4) ◽  
pp. 585-594 ◽  
Author(s):  
T. J. Bouma ◽  
R. De Visser ◽  
J. H. J. A. Janssen ◽  
M. J. De Kock ◽  
P H. Van Leeuwen ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Protein Turnover ◽  
Energy Requirements ◽  
Inhibitor Of Protein Synthesis

Paroxetine treatment alters hippocampal protein turnover

2017 ◽  
Author(s):  
M Filiou ◽  
C Guillermier ◽  
C Poczatek ◽  
M Wang ◽  
A Chen ◽  
...  
Keyword(s):  
Protein Turnover ◽  
Paroxetine Treatment
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