Functional Expression of Plant Membrane Proteins in Lactococcus lactis

Author(s):  
Sylvain Boutigny ◽  
Emeline Sautron ◽  
Annie Frelet-Barrand ◽  
Lucas Moyet ◽  
Daniel Salvi ◽  
...  
Author(s):  
Sana Bakari ◽  
François André ◽  
Daphné Seigneurin-Berny ◽  
Marcel Delaforge ◽  
Norbert Rolland ◽  
...  

Science ◽  
2012 ◽  
Vol 337 (6090) ◽  
pp. 93-96 ◽  
Author(s):  
Sébastien Herzig ◽  
Etienne Raemy ◽  
Sylvie Montessuit ◽  
Jean-Luc Veuthey ◽  
Nicola Zamboni ◽  
...  

The transport of pyruvate, the end product of glycolysis, into mitochondria is an essential process that provides the organelle with a major oxidative fuel. Although the existence of a specific mitochondrial pyruvate carrier (MPC) has been anticipated, its molecular identity remained unknown. We report that MPC is a heterocomplex formed by two members of a family of previously uncharacterized membrane proteins that are conserved from yeast to mammals. Members of the MPC family were found in the inner mitochondrial membrane, and yeast mutants lacking MPC proteins showed severe defects in mitochondrial pyruvate uptake. Coexpression of mouse MPC1 and MPC2 in Lactococcus lactis promoted transport of pyruvate across the membrane. These observations firmly establish these proteins as essential components of the MPC.


PLoS ONE ◽  
2010 ◽  
Vol 5 (1) ◽  
pp. e8746 ◽  
Author(s):  
Annie Frelet-Barrand ◽  
Sylvain Boutigny ◽  
Lucas Moyet ◽  
Aurélien Deniaud ◽  
Daphné Seigneurin-Berny ◽  
...  

2012 ◽  
Vol 54 (5) ◽  
pp. 404-409 ◽  
Author(s):  
G. Gao ◽  
J.-J. Qiao ◽  
C.-H. Yang ◽  
D.-Z. Jiang ◽  
R.-Q. Li ◽  
...  

2010 ◽  
Vol 401 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Daniel M. Linares ◽  
Eric R. Geertsma ◽  
Bert Poolman

2014 ◽  
Vol 426 (1) ◽  
pp. 136-149 ◽  
Author(s):  
Nadia Gul ◽  
Daniel M. Linares ◽  
Franz Y. Ho ◽  
Bert Poolman

2010 ◽  
Vol 27 (4) ◽  
pp. 283-288 ◽  
Author(s):  
Siqing Liu ◽  
Kenneth M. Bischoff ◽  
Nasib Qureshi ◽  
Steven R. Hughes ◽  
Joseph O. Rich

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e21873 ◽  
Author(s):  
Joao P. C. Pinto ◽  
Oscar P. Kuipers ◽  
Ravi K. R. Marreddy ◽  
Bert Poolman ◽  
Jan Kok

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