Rapid Mitochondrial DNA Isolation Method for Direct Sequencing

2015 ◽  
pp. 89-95 ◽  
Author(s):  
Wilber Quispe-Tintaya ◽  
Ryan R. White ◽  
Vasily N. Popov ◽  
Jan Vijg ◽  
Alexander Y. Maslov
Keyword(s):  
Mitochondrial Dna ◽  
Dna Isolation ◽  
Direct Sequencing ◽  
Isolation Method

scholarly journals Biased nicking of mitochondrial DNA during extraction can be avoided by choice of isolation method

2021 ◽  
Author(s):  
Bruno Marçal Repolês ◽  
Choco Michael Gorospe ◽  
Phong Tran ◽  
Anna Karin Nilsson ◽  
Paulina H. Wanrooij
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Dna ◽  
Dna Isolation ◽  
Isolation Procedure ◽  
Isolation Method ◽  
Strand Breaks ◽  
Careful Choice ◽  
Isolation Methods ◽  
Solid Tissues ◽  
Long Range Pcr

AbstractThe integrity of mitochondrial DNA (mtDNA) isolated from solid tissues is critical for analyses such as long-range PCR. We show that a commonly-used DNA isolation procedure preferentially introduces strand breaks into the mtDNA extracted from the skeletal muscle of aged mice, while mtDNA from adult animals is less affected. We present a comparison of mtDNA isolation methods and identify one that avoids this biased loss of muscle mtDNA integrity. Our results highlight the importance of a careful choice of mtDNA isolation method and serve as a resource to researchers planning analysis of mtDNA isolated from solid tissues.

scholarly journals Két generációban megfigyelhető mitokondriális DNS A8344G mutáció

2017 ◽  
Vol 158 (12) ◽  
pp. 468-471 ◽  
Author(s):  
Anett Fekete ◽  
Kinga Hadzsiev ◽  
Judit Bene ◽  
Antónia Nászai ◽  
Petra Mátyás ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Isolation ◽  
Trna Gene ◽  
Clinical Phenotype ◽  
Direct Sequencing ◽  
Mitochondrial Diseases ◽  
Adult Onset ◽  
Definite Diagnosis ◽  
Investigation Period ◽  
Merrf Syndrome

Abstract: This article presents the case of a 62-year-old mother and her 41-year-old daughter, who have had severe neurological symptoms for a few decades. After a long investigation period the definite diagnosis of MERRF syndrome was confirmed. After DNA isolation from our patient’s blood sample we examined the mitochondrial DNA with direct sequencing. An adenine-guanine substitution was detected in the tRNA gene at position 8344, based on the sequence ferogram the heteroplasmy was over 90%. The clinical phenotype was not clearly characteristic for MERRF syndrome, adult-onset and lipomas are not typical in this disease. In our case report we would like to draw attention to the great phenotypic variation of the mitochondrial diseases and we emphasize that these disorders are underdiagnosed in Hungary even today. Orv. Hetil., 2017, 158(12), 468–471.

scholarly journals Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA

1987 ◽  
Vol 15 (2) ◽  
pp. 529-541 ◽  
Author(s):  
Lisa A. Wrischnik ◽  
Russell G. Higuchi ◽  
Mark Stoneking ◽  
Henry A. Erlich ◽  
Norman Arnheim ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Direct Sequencing ◽  
Human Mitochondrial Dna ◽  
Length Mutations

Methods for Detection of Anticarsia gemmatalis Nucleopolyhedrovirus DNA in Soil

1999 ◽  
Vol 65 (6) ◽  
pp. 2307-2311 ◽  
Author(s):  
R. R. de Moraes ◽  
J. E. Maruniak ◽  
J. E. Funderburk
Keyword(s):  
Laboratory Experiments ◽  
Dna Isolation ◽  
Pcr Amplification ◽  
Soil Samples ◽  
Anticarsia Gemmatalis ◽  
Isolation Method ◽  
Viral Dna ◽  
Pcr Inhibitors ◽  
Magnetic Capture ◽  
Pcr Products

ABSTRACT Two methods, phenol-ether and magnetic capture-hybridization (MCH), were developed and compared with regard to their sensitivities and abilities to extract the DNA of the insect baculovirus Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) from soil and to produce DNA amplifiable by PCR. Laboratory experiments were performed with 0.25 g of autoclaved soil inoculated with different viral concentrations to optimize both methods of baculovirus DNA extraction and to determine their sensitivities. Both procedures produced amplifiable DNA; however, the MCH method was 100-fold more sensitive than the phenol-ether procedure. The removal of PCR inhibitors from the soil appeared to be complete when MCH was used as the viral DNA isolation method, because undiluted aliquots of the DNA preparations could be amplified by PCR. The phenol-ether procedure probably did not completely remove PCR inhibitors from the soil, since PCR products were observed only when the AgMNPV DNA preparations were diluted 10- or 100-fold. AgMNPV DNA was detected in field-collected soil samples from 15 to 180 days after virus application when the MCH procedure to isolate DNA was coupled with PCR amplification of the polyhedrin region.

DNA isolation method for high polysaccharide Lesquerella species

1999 ◽  
Vol 9 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Benjamin Kaufman ◽  
Stacy Richards ◽  
David A Dierig
Keyword(s):  
Dna Isolation ◽  
Isolation Method

High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples

2011 ◽  
Vol 86 (3) ◽  
pp. 310-312 ◽  
Author(s):  
Jeff A. Brady ◽  
Jennifer B. Faske ◽  
Jessica M. Castañeda-Gill ◽  
Jonathan L. King ◽  
Forrest L. Mitchell
Keyword(s):  
High Throughput ◽  
Dna Isolation ◽  
Xylella Fastidiosa ◽  
Isolation Method
Sign in / Sign up

Export Citation Format

Share Document