Detection of Cell Death and Phagocytosis in the Drosophila Ovary

2015 ◽  
pp. 191-206 ◽  
Author(s):  
Tracy L. Meehan ◽  
Alla Yalonetskaya ◽  
Tony F. Joudi ◽  
Kimberly McCall
Keyword(s):  
Cell Death ◽  
Drosophila Ovary

scholarly journals Mitochondrial regulation of cell death in the Drosophila ovary

Autophagy ◽  
2011 ◽  
Vol 7 (7) ◽  
pp. 793-794 ◽  
Author(s):  
Elizabeth A. Tanner ◽  
Kim McCall
Keyword(s):  
Cell Death ◽  
Mitochondrial Regulation ◽  
Drosophila Ovary

scholarly journals The axon guidance gene lola is required for programmed cell death in the Drosophila ovary

2007 ◽  
Vol 304 (2) ◽  
pp. 771-785 ◽  
Author(s):  
B. Paige Bass ◽  
Kristen Cullen ◽  
Kimberly McCall
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Axon Guidance ◽  
Drosophila Ovary

scholarly journals An RNAi screen of the kinome in epithelial follicle cells of the Drosophila melanogaster ovary reveals genes required for proper germline death and clearance

2021 ◽  
Vol 11 (2) ◽  
Author(s):  
Diane P V Lebo ◽  
Alice Chirn ◽  
Jeffrey D Taylor ◽  
Andre Levan ◽  
Valentina Doerre Torres ◽  
...  
Keyword(s):  
Cell Death ◽  
Drosophila Melanogaster ◽  
Follicle Cells ◽  
Multiple Time ◽  
Cell Clearance ◽  
Germline Cells ◽  
Multiple Time Points ◽  
Cell Corpse ◽  
Cell Corpse Clearance ◽  
Drosophila Ovary

Abstract Programmed cell death and cell corpse clearance are an essential part of organismal health and development. Cell corpses are often cleared away by professional phagocytes such as macrophages. However, in certain tissues, neighboring cells known as nonprofessional phagocytes can also carry out clearance functions. Here, we use the Drosophila melanogaster ovary to identify novel genes required for clearance by nonprofessional phagocytes. In the Drosophila ovary, germline cells can die at multiple time points. As death proceeds, the epithelial follicle cells act as phagocytes to facilitate the clearance of these cells. We performed an unbiased kinase screen to identify novel proteins and pathways involved in cell clearance during two death events. Of 224 genes examined, 18 demonstrated severe phenotypes during developmental death and clearance while 12 demonstrated severe phenotypes during starvation-induced cell death and clearance, representing a number of pathways not previously implicated in phagocytosis. Interestingly, it was found that several genes not only affected the clearance process in the phagocytes, but also non-autonomously affected the process by which germline cells died. This kinase screen has revealed new avenues for further exploration and investigation.

scholarly journals Eggs over easy: cell death in the Drosophila ovary

2004 ◽  
Vol 274 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Kimberly McCall
Keyword(s):  
Cell Death ◽  
Drosophila Ovary

scholarly journals Murder on the Ovarian Express: A Tale of Non-Autonomous Cell Death in the Drosophila Ovary

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1454
Author(s):  
Diane Patricia Vig Lebo ◽  
Kimberly McCall
Keyword(s):  
Cell Death ◽  
Follicle Cells ◽  
Nurse Cells ◽  
Fine Line ◽  
Egg Chambers ◽  
Drosophila Ovary ◽  
Size And Structure

Throughout oogenesis, Drosophila egg chambers traverse the fine line between survival and death. After surviving the ten early and middle stages of oogenesis, egg chambers drastically change their size and structure to produce fully developed oocytes. The development of an oocyte comes at a cost, the price is the lives of the oocyte’s 15 siblings, the nurse cells. These nurse cells do not die of their own accord. Their death is dependent upon their neighbors—the stretch follicle cells. Stretch follicle cells are nonprofessional phagocytes that spend the final stages of oogenesis surrounding the nurse cells and subsequently forcing the nurse cells to give up everything for the sake of the oocyte. In this review, we provide an overview of cell death in the ovary, with a focus on recent findings concerning this phagocyte-dependent non-autonomous cell death.

scholarly journals Bcl-2 proteins and autophagy regulate mitochondrial dynamics during programmed cell death in the Drosophila ovary

Development ◽  
2010 ◽  
Vol 138 (2) ◽  
pp. 327-338 ◽  
Author(s):  
E. A. Tanner ◽  
T. A. Blute ◽  
C. B. Brachmann ◽  
K. McCall
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Mitochondrial Dynamics ◽  
Drosophila Ovary

scholarly journals Cracking open cell death in the Drosophila ovary

APOPTOSIS ◽  
2009 ◽  
Vol 14 (8) ◽  
pp. 969-979 ◽  
Author(s):  
Tracy L. Pritchett ◽  
Elizabeth A. Tanner ◽  
Kimberly McCall
Keyword(s):  
Cell Death ◽  
Open Cell ◽  
Drosophila Ovary

scholarly journals Phagocytosis genes nonautonomously promote developmental cell death in the Drosophila ovary

2016 ◽  
Vol 113 (9) ◽  
pp. E1246-E1255 ◽  
Author(s):  
Allison K. Timmons ◽  
Albert A. Mondragon ◽  
Claire E. Schenkel ◽  
Alla Yalonetskaya ◽  
Jeffrey D. Taylor ◽  
...  
Keyword(s):  
Cell Death ◽  
Large Scale ◽  
Germ Line ◽  
Follicle Cells ◽  
Small Subset ◽  
Nurse Cells ◽  
Cell Death Program ◽  
Cell Death Mechanisms ◽  
Developmental Cell Death ◽  
Drosophila Ovary

Programmed cell death (PCD) is usually considered a cell-autonomous suicide program, synonymous with apoptosis. Recent research has revealed that PCD is complex, with at least a dozen cell death modalities. Here, we demonstrate that the large-scale nonapoptotic developmental PCD in the Drosophila ovary occurs by an alternative cell death program where the surrounding follicle cells nonautonomously promote death of the germ line. The phagocytic machinery of the follicle cells, including Draper, cell death abnormality (Ced)-12, and c-Jun N-terminal kinase (JNK), is essential for the death and removal of germ-line–derived nurse cells during late oogenesis. Cell death events including acidification, nuclear envelope permeabilization, and DNA fragmentation of the nurse cells are impaired when phagocytosis is inhibited. Moreover, elimination of a small subset of follicle cells prevents nurse cell death and cytoplasmic dumping. Developmental PCD in the Drosophila ovary is an intriguing example of nonapoptotic, nonautonomous PCD, providing insight on the diversity of cell death mechanisms.

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