Fluorimetric Methods for Analysis of Permeability, Drug Transport Kinetics, and Inhibition of the ABCB1 Membrane Transporter

2016 ◽  
pp. 87-103 ◽  
Author(s):  
Ana Armada ◽  
Célia Martins ◽  
Gabriella Spengler ◽  
Joseph Molnar ◽  
Leonard Amaral ◽  
...  
Keyword(s):  
Drug Transport ◽  
Transport Kinetics ◽  
Membrane Transporter

Analysis of Drug Transport Kinetics in Multidrug-Resistant Cells Using a Novel Coumarin-Vinblastine Compound

Biochemistry ◽  
1994 ◽  
Vol 33 (42) ◽  
pp. 12665-12675 ◽  
Author(s):  
William G. Bornmann ◽  
Paul D. Roepe
Keyword(s):  
Drug Transport ◽  
Multidrug Resistant ◽  
Transport Kinetics ◽  
Resistant Cells

Characterization and modulation of drug transport kinetics in K562 cl.6 daunorubicin-resistant cell line

1994 ◽  
Vol 86 (3) ◽  
pp. 547-554 ◽  
Author(s):  
Xu-Rong Jiang ◽  
Marion G. Macey ◽  
Peter W. Collins ◽  
Adrian C. Newland
Keyword(s):  
Cell Line ◽  
Drug Transport ◽  
Resistant Cell ◽  
Resistant Cell Line ◽  
Transport Kinetics

Identification of Human Cd8+ and Cd4+ Lymphocytes with Unique Drug Resistance Activity by Imaging the Drug Transport Kinetics at the Single Cell Resolution.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3894-3894
Author(s):  
Orian Shirihai ◽  
Israel Biran ◽  
Kirk M. Bertelsen ◽  
David J. Greenblatt ◽  
Motti Deutsch ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Drug Transport ◽  
Honeycomb Structure ◽  
Transport Kinetics ◽  
Kinetic Measurements ◽  
Imaging Device ◽  
Cd8 Cells ◽  
P Glycoprotein ◽  
The Individual ◽  
Cd4 Lymphocytes

Abstract Drug resistance activity of transporters such as P-glycoprotein within lymphocytes may have significant consequences to the pharmacologic treatment of HIV, immune suppression, autoimmune diseases, and leukemia, as many drugs are substrates of the MDR transporters. Variability in MDR activity may be a significant barrier in the evaluation of the predicted effect of a drug and on the therapeutic dosage necessary to overcome MDR activity. Current methodologies to measure MDR, e.g. FACS, do not provide kinetic measurements of dye efflux at the resolution of the individual cell thereby missing the existence of functionally different subpopulations. To overcome these limitations, we developed a new imaging device that is comprised of thousands of transparent pico-wells (20 micron in diameter) organized in a honeycomb structure that enables real-time visualization and sequential quantification of drug transport rate from thousands of individual living lymphocytes, thereby generating MDR kinetic data. Fluorescence microscopy was used to measure DiOC2 dye (a common fluorescent probe for P-glycoprotein transport) retention from individual human CD4+ or CD8+ lymphocytes over time. Kinetic analysis of dye export from CD4+ and CD8+ cells revealed the occurrence of subpopulations of cells with unique sensitivity to MDR inhibitors. When the MDR inhibitor Verapamil was used, CD8+ lymphocytes demonstrated a greater magnitude of inhibition compared to CD4+ lymphocytes. Remarkably, CD4+ lymphocytes contained a sizable subpopulation which was verapamil insensitive, suggesting the existence of a non-P-glycoprotein drug export activity in these cells. These findings indicate that functional MDR assays, which are based on transport kinetics, can efficiently dissect the heterogeneous lymphocyte cell populations and identify unique subsets of cells with increased activity. Figure Figure

Effect of controlled laser microporation on drug transport kinetics into and across the skin

2010 ◽  
Vol 146 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Y.G. Bachhav ◽  
S. Summer ◽  
A. Heinrich ◽  
T. Bragagna ◽  
C. Böhler ◽  
...  
Keyword(s):  
Drug Transport ◽  
Transport Kinetics

scholarly journals Does fed‐state associated prolonged gastric emptying affect drug transport kinetics in intestine?

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Sheena Sharma ◽  
Clark Kogan ◽  
Bhagwat Prasad
Keyword(s):  
Gastric Emptying ◽  
Drug Transport ◽  
Transport Kinetics ◽  
Fed State

Analysis of Drug Transport Kinetics in Multidrug-resistant Cells: Implications for Drug Action

2001 ◽  
Vol 8 (1) ◽  
pp. 51-64 ◽  
Author(s):  
Arlette Garnier-Suillerot ◽  
Carole Marbeuf-Gueye ◽  
Milena Salerno ◽  
Chatchanok Loetchutinat ◽  
Izabela Fokt ◽  
...  
Keyword(s):  
Drug Transport ◽  
Multidrug Resistant ◽  
Drug Action ◽  
Transport Kinetics ◽  
Resistant Cells
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