Studying Antibody Repertoires with Next-Generation Sequencing

2016 ◽  
pp. 257-270 ◽  
Author(s):  
William D. Lees ◽  
Adrian J. Shepherd
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Antibody Repertoires ◽  
Generation Sequencing

scholarly journals Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

10.3791/58804 ◽  
2019 ◽  
Author(s):  
Lin Sun ◽  
Naoko Kono ◽  
Hiroyuki Toh ◽  
Hanbing Xue ◽  
Kaori Sano ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Human Antibody ◽  
Next Generation ◽  
Antibody Repertoires ◽  
Generation Sequencing

scholarly journals Observed Antibody Space: a resource for data mining next generation sequencing of antibody repertoires

2018 ◽  
Author(s):  
Aleksandr Kovaltsuk ◽  
Jinwoo Leem ◽  
Sebastian Kelm ◽  
James Snowden ◽  
Charlotte M. Deane ◽  
...  
Keyword(s):  
Data Mining ◽  
Immune System ◽  
Next Generation Sequencing ◽  
Sequence Diversity ◽  
Immunoglobulin Gene ◽  
Next Generation ◽  
Natural Diversity ◽  
Antibody Repertoires ◽  
Generation Sequencing

AbstractAntibodies are immune system proteins that recognize noxious molecules for elimination. Their sequence diversity and binding versatility have made antibodies the primary class of biopharmaceuticals. Recently it has become possible to query their immense natural diversity using next-generation sequencing of immunoglobulin gene repertoires (Ig-seq). However, Ig-seq outputs are currently fragmented across repositories and tend to be presented as raw nucleotide reads, which means nontrivial effort is required to reuse the data for analysis. To address this issue, we have collected Ig-seq outputs from 53 studies, covering more than half a billion antibody sequences across diverse immune states, organisms and individuals. We have sorted, cleaned, annotated, translated and numbered these sequences and make the data available via our Observed Antibody Space (OAS) resource at antibodymap.org. The data within OAS will be regularly updated with newly released Ig-seq datasets. We believe OAS will facilitate data mining of immune repertoires for improved understanding of the immune system and development of better biotherapeutics.

scholarly journals Observed Antibody Space: A Resource for Data Mining Next-Generation Sequencing of Antibody Repertoires

2018 ◽  
Vol 201 (8) ◽  
pp. 2502-2509 ◽  
Author(s):  
Aleksandr Kovaltsuk ◽  
Jinwoo Leem ◽  
Sebastian Kelm ◽  
James Snowden ◽  
Charlotte M. Deane ◽  
...  
Keyword(s):  
Data Mining ◽  
Next Generation Sequencing ◽  
Next Generation ◽  
Antibody Repertoires ◽  
Generation Sequencing

Next-Generation Sequencing

2012 ◽  
Vol 42 (9) ◽  
pp. 8
Author(s):  
PETER HULICK
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Generation Sequencing
Sign in / Sign up

Export Citation Format

Share Document