Loop-Mediated Isothermal Amplification and LFD Combination for Detection of Plasmodium falciparum and Plasmodium vivax

2017 ◽  
pp. 431-443 ◽  
Author(s):  
Darin Kongkasuriyachai ◽  
Suganya Yongkiettrakul ◽  
Wansika Kiatpathomchai ◽  
Narong Arunrut
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

Detection of Plasmodium vivax and Plasmodium falciparum DNA in human saliva and urine: Loop-mediated isothermal amplification for malaria diagnosis

Acta Tropica ◽  
2014 ◽  
Vol 136 ◽  
pp. 44-49 ◽  
Author(s):  
Zahra Ghayour Najafabadi ◽  
Hormozd Oormazdi ◽  
Lame Akhlaghi ◽  
Ahmad Reza Meamar ◽  
Mehdi Nateghpour ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Malaria Diagnosis ◽  
Human Saliva ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax

2014 ◽  
Vol 63 (6) ◽  
pp. 777-784 ◽  
Author(s):  
Suganya Yongkiettrakul ◽  
Wansadaj Jaroenram ◽  
Narong Arunrut ◽  
Wanwisa Chareanchim ◽  
Supicha Pannengpetch ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Lateral Flow Dipstick ◽  
Amplification Assay

scholarly journals Development of Two-Tube Loop-Mediated Isothermal Amplification Assay for Differential Diagnosis of Plasmodium falciparum and Plasmodium vivax and Its Comparison with Loopamp™ Malaria

Diagnostics ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1689
Author(s):  
Mudsser Azam ◽  
Kirti Upmanyu ◽  
Ratan Gupta ◽  
Karugatharayil Sasi Sruthy ◽  
Monika Matlani ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Plasmodium Vivax ◽  
Sensitivity And Specificity ◽  
Limit Of Detection ◽  
Lamp Assay ◽  
Repeat Sequence ◽  
Isothermal Amplification ◽  
Malaria Surveillance ◽  
Loop Mediated Isothermal Amplification ◽  
Closed Tube

To strengthen malaria surveillance, field-appropriate diagnostics requiring limited technical resources are of critical significance. Loop-mediated isothermal amplification (LAMP) based malaria diagnostic assays are potential point-of-care tests with high sensitivity and specificity and have been used in low-resource settings. Plasmodium vivax–specific consensus repeat sequence (CRS)-based and Plasmodium falciparum–specific 18S rRNA primers were designed, and a two-tube LAMP assay was developed. The diagnostic performance of a closed-tube LAMP assay and Loopamp™ Malaria Detection (Pan/Pf, Pv) kit was investigated using nested PCR confirmed mono- and co-infections of P. vivax and P. falciparum positive (n = 149) and negative (n = 67) samples. The closed-tube Pv LAMP assay showed positive amplification in 40 min (limit of detection, LOD 0.7 parasites/µL) and Pf LAMP assay in 30 min (LOD 2 parasites/µL). Pv LAMP and Pf LAMP demonstrated a sensitivity and specificity of 100% (95% CI, 95.96–100% and 89.85–100%, respectively). The LoopampTM Pan/Pf Malaria Detection kit demonstrated a sensitivity and specificity of 100%, whereas LoopampTM Pv showed a sensitivity of 98.36% (95% CI, 91.28–99.71%) and specificity of 100% (95% CI, 87.54–100%). The developed two-tube LAMP assay is highly sensitive (LOD ≤ 2 parasite/µL), demonstrating comparable results with the commercial Loopamp™ Malaria Detection (Pf/pan) kit, and was superior in detecting the P. vivax co-infection that remained undetected by the Loopamp™ Pv kit. The developed indigenous two-tube Pf/Pv malaria detection can reliably be used for mass screening in resource-limited areas endemic for both P. falciparum and P. vivax malaria.

Heat-enhancing aggregation of Gold nanoparticles combined with loop-mediated isothermal amplification (HAG-LAMP) for Plasmodium falciparum detection

2021 ◽  
pp. 114178
Author(s):  
Patcharapan Suwannin ◽  
Duangporn Polpanich ◽  
Saovanee Leelayoova ◽  
Mathirut Mungthin ◽  
Pramuan Tangboriboonrat ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Plasmodium Falciparum ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

scholarly journals Real-Time Loop-Mediated Isothermal Amplification (RealAmp) for the Species-Specific Identification of Plasmodium vivax

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54986 ◽  
Author(s):  
Jaymin C. Patel ◽  
Jenna Oberstaller ◽  
Maniphet Xayavong ◽  
Jothikumar Narayanan ◽  
Jeremy D. DeBarry ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Real Time ◽  
Isothermal Amplification ◽  
Specific Identification ◽  
Loop Mediated Isothermal Amplification ◽  
Species Specific ◽  
Time Loop

scholarly journals Evaluation of Loop Mediated Isothermal Amplification (LAMP) for Diagnosis of Plasmodium falciparum in Wad Medani City, Gezira State, Sudan

2019 ◽  
pp. 1-7
Author(s):  
M. Y. Mohamed ◽  
A. D. Abakar ◽  
B. A. Talha ◽  
Salah Eldin G. Elzaki ◽  
Y. A. Mohammed ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Diagnostic Performance ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Teaching Hospitals ◽  
Molecular Technique ◽  
Rrna Gene ◽  
Predictive Values ◽  
Loop Mediated Isothermal Amplification

Plasmodium falciparum considered as the most serious form of species causes malaria compared with other species. Diagnosis of falciparum malaria in Sudan remain a major problem, the laboratory diagnosis depends solely on microscopy and RDTs. Loop mediated isothermal amplification (LAMP) assay is a molecular technique done in isothermal temperature using simple, inexpensive instruments for detection of falciparum malaria. The aim of the study is to evaluate the diagnostic performance of loop mediated isothermal amplification (LAMP) assay for detection of P. falciparum and compare with microscopic detection. A cross sectional hospital based study conducted on 220 blood samples collected from participants suspected to have falciparum malaria attending Wad Medani Teaching Hospitals and 26 healthy participants during the period November 2018 to January 2019. Thick blood films were done and used for P. falciparum detection. The extracted DNA by TE buffer was amplified by LAMP assay targeting 18S rRNA gene. Data were analyzed using Medical calculator (MedCalc) programs (V. 16). The results showed that the sensitivity, specificity, positive predictive value, negative predictive values were 99.1%, 84.6%, 53.2%, 99.8% respectively. Validation of LAMP diagnostic performance revealed that area under the curve is 0.919, while Weighted Kappa is 0.866. The study concluded that the LAMP assay had the identical diagnostic performance compared with microscopy in diagnosis of Plasmodium falciparum malaria. This gives a relative effortlessness application of LAMP assay in Sudan after availing the required logistics.

scholarly journals Diagnosis of Plasmodium vivax by Loop-Mediated Isothermal Amplification in Febrile Patient Samples from Loreto, Perú

2020 ◽  
Vol 103 (4) ◽  
pp. 1549-1552
Author(s):  
Oscar Nolasco ◽  
Beronica Infante ◽  
Juan Contreras-Mancilla ◽  
Sandra Incardona ◽  
Xavier C. Ding ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Isothermal Amplification ◽  
Febrile Patient ◽  
Loop Mediated Isothermal Amplification
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