Secreted Reporters for Monitoring Multiple Promoter Function

2017 ◽  
pp. 33-47 ◽  
Author(s):  
Ghazal Lashgari ◽  
Rami S. Kantar ◽  
Bakhos A. Tannous
Keyword(s):  
Promoter Function ◽  
Multiple Promoter

Regulation of the vhs Gene Promoter of Pseudorabies Virus by IE180 and EP0, and the Requirement of a Sp1 Site for the Promoter Function

Virus Genes ◽  
2004 ◽  
Vol 28 (3) ◽  
pp. 247-258 ◽  
Author(s):  
Yuan-Yen Chang ◽  
Hui-Wen Lin ◽  
Min-Liang Wong ◽  
Tien-Jye Chang
Keyword(s):  
Pseudorabies Virus ◽  
Gene Promoter ◽  
Promoter Function

scholarly journals Diphtheria toxin promoter function inCorynebacterium diphtheriaeandEscherichia coli

1985 ◽  
Vol 13 (9) ◽  
pp. 3147-3159 ◽  
Author(s):  
M. Kaczorek ◽  
G. Zettlmeissl ◽  
F. Delpeyroux ◽  
R.E. Streeck
Keyword(s):  
Diphtheria Toxin ◽  
Promoter Function

Sensitive fluorescent microplate bioassay using recombinant Escherichia coli with multiple promoter–reporter units in tandem for detection of arsenic

2009 ◽  
Vol 108 (5) ◽  
pp. 414-420 ◽  
Author(s):  
Chiaki Tani ◽  
Koichi Inoue ◽  
Yuri Tani ◽  
Md. Harun-ur-Rashid ◽  
Norihiro Azuma ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Escherichia Coli ◽  
Multiple Promoter

Transient expression of a mouse alpha-fetoprotein minigene: deletion analyses of promoter function

1983 ◽  
Vol 3 (7) ◽  
pp. 1295-1309
Author(s):  
R W Scott ◽  
S M Tilghman
Keyword(s):  
Transient Expression ◽  
Hela Cells ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Alpha Fetoprotein ◽  
Tata Box ◽  
Deletion Mutants ◽  
Promoter Function ◽  
Flanking Region

The constitutive transcription of a mouse alpha-fetoprotein (AFP) minigene was examined during the transient expression of AFP-simian virus 40-pBR322 recombinant DNAs introduced into HeLa cells by Ca3(PO4)2 precipitation. We tested three constructs, each of which contains the AFP minigene and pBR322 DNAs inserted in the late region of simian virus 40 and found that the relative efficiency of AFP gene expression was dependent on the arrangement of the three DNA elements in the vector. The transcripts begin at the authentic AFP cap site and are properly spliced and polyadenylated. To define a sequence domain in the 5' flanking region of the AFP gene required for constitutive expression, sequential 5' deletion mutants of the AFP minigene were constructed and introduced into HeLa cells. All AFP deletion mutants which retained at least the TATA motif located 30 base pairs upstream from the cap site were capable of directing accurate and efficient AFP transcription. However, when the TATA sequence was deleted, no accurately initiated AFP transcripts were detected. These results are identical to those obtained from in vitro transcription of truncated AFP 5' deletion mutant templates assayed in HeLa cell extracts. The rate of AFP transcription in vivo was unaffected by deletion of DNA upstream of the AFP TATA box but was greatly affected by the distance between the simian virus 40 control region and the 5' end of the gene. The absence of any promoter activity upstream of the TATA box in this assay system is in contrast to what has been reported for several other eucaryotic structural genes in a variety of in vivo systems. A sequence comparison between the 5' flanking region of the AFP gene and these genes suggested that the AFP gene lacks those structural elements found to be important for constitutive transcription in vivo. Either the AFP gene lacks upstream promoter function in the 5' flanking DNA contained within the minigene, or the use of a viral vector in a heterologous system precludes its identification.

Secretory overexpression of the endoglucanase by Saccharomyces cerevisiae via CRISPR-δ-integration and multiple promoter shuffling

2019 ◽  
Vol 121 ◽  
pp. 17-22 ◽  
Author(s):  
Yuka Sasaki ◽  
Ryosuke Mitsui ◽  
Ryosuke Yamada ◽  
Hiroyasu Ogino
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Multiple Promoter

scholarly journals Transcription of the Murine Interleukin 5 Gene Is Regulated by Multiple Promoter Elements

1995 ◽  
Vol 270 (35) ◽  
pp. 20575-20582 ◽  
Author(s):  
Kimberly S. Stranick ◽  
Faribourz Payvandi ◽  
Demetris N. Zambas ◽  
Shelby P. Umland ◽  
Robert W. Egan ◽  
...  
Keyword(s):  
Promoter Elements ◽  
Interleukin 5 ◽  
Multiple Promoter
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