Abstract
Background: Tiller number is a factor determining panicle number and grain yield in wheat (Triticum aestivum L.). Auxin plays an important role in the regulation of branch production. PIN-FORMED 1 (PIN1), an auxin efflux carrier, plays a role in the regulation of tiller number in rice (Oryza sativa); however, little is known on the roles of PIN1 in wheat. Results: Nine homologs of TaPIN1 genes were identified in wheat, of which TaPIN1-6 genes showed higher expression in the stem apex and young leaf in wheat, and the TaPIN1-6a protein was localized in the plasma membrane. The down-expression of TaPIN1s increased the tiller number in TaPIN1-RNA interference (TaPIN1-RNAi) transgenic wheat plants, indicating that auxin might mediate the axillary bud production. By contrast, the spikelet number, grain number per panicle, and the 1000-grain weight were decreased in the TaPIN1-RNAi transgenic wheat plants compared with those in the wild type. Conclusions: Phylogenetic analysis and expression patterns of nine TaPIN1 genes, and their protein structures and subcellular localization of TaPIN1-6a protein were analyzed. Down-regulated expression of TaPIN1 genes increased the tiller numbers of transgenic wheat lines. Our study suggests that TaPIN1s is required for the regulation of grain yield in wheat.
A biolistic method for high-throughput production of transgenic wheat plants with single gene insertions