bipolaris sorokiniana
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2022 ◽  
Vol 354 (11-12) ◽  
pp. 82-87
Author(s):  
A. L. Toygildin ◽  
M. I. Podsevalov ◽  
I. A. Toygildina ◽  
V. N. Austin

In the conditions of the forest-steppe zone of the Volga region, winter wheat occupies the largest area among grain crops, which is associated with its relatively high productivity, economic efficiency and the ability to unload the peaks of field work in the spring. Winter wheat makes fuller use of the bioclimatic potential of productivity, especially in the arid conditions of the region. Despite the fact that the adaptive-integrated approach to the joint use of agricultural techniques and chemicalization means when growing grain crops provides significant and stable yield increases, they require regular study to adjust agricultural technologies. The article presents the results of 3-year studies in a stationary field experiment to assess the influence of predecessors, methods of basic soil cultivation and plant protection on the spread of diseases and weeds, as well as on the yield of winter wheat. Studies have shown that the placement of winter wheat after clean fallow helps to reduce the infestation of crops, and after cruciferous predecessors (white mustard, spring rape)— the spread of root rot (Bipolaris sorokiniana (Helmintosporium sativum)) and leaf rust (Puccinia recondita). Winter wheat yield after different predecessors varied from 5.16 t/ha for pure fallow to 3.77–4.04 t/ha after nonfallow predecessors with a significant increase in adaptive-integrated plant protection by 0.37 t/ha (9.2%) and insignificant — for combined tillage in crop rotation — by 0.19 t/ha (4.6%).


2022 ◽  
Vol 17 (1) ◽  
pp. 152-167
Author(s):  
Muhammad Junaid Yousaf ◽  
Anwar Hussain ◽  
Muhammad Hamayun ◽  
Amjad Iqbal ◽  
In-Jung Lee

PLoS Genetics ◽  
2021 ◽  
Vol 17 (12) ◽  
pp. e1009473
Author(s):  
Gazala Ameen ◽  
Shyam Solanki ◽  
Lauren Sager-Bittara ◽  
Jonathan Richards ◽  
Prabin Tamang ◽  
...  

Disease lesion mimic mutants (DLMMs) are characterized by the spontaneous development of necrotic spots with various phenotypes designated as necrotic (nec) mutants in barley. The nec mutants were traditionally considered to have aberrant regulation of programmed cell death (PCD) pathways, which have roles in plant immunity and development. Most barley nec3 mutants express cream to orange necrotic lesions contrasting them from typical spontaneous DLMMs that develop dark pigmented lesions indicative of serotonin/phenolics deposition. Barley nec3 mutants grown under sterile conditions did not exhibit necrotic phenotypes until inoculated with adapted pathogens, suggesting that they are not typical DLMMs. The F2 progeny of a cross between nec3-γ1 and variety Quest segregated as a single recessive susceptibility gene post-inoculation with Bipolaris sorokiniana, the causal agent of the disease spot blotch. Nec3 was genetically delimited to 0.14 cM representing 16.5 megabases of physical sequence containing 149 annotated high confidence genes. RNAseq and comparative analysis of the wild type and five independent nec3 mutants identified a single candidate cytochrome P450 gene (HORVU.MOREX.r2.6HG0460850) that was validated as nec3 by independent mutations that result in predicted nonfunctional proteins. Histology studies determined that nec3 mutants had an unstable cutin layer that disrupted normal Bipolaris sorokiniana germ tube development.


Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1526
Author(s):  
Yanjie Yi ◽  
Youtian Shan ◽  
Shifei Liu ◽  
Yanhui Yang ◽  
Yang Liu ◽  
...  

Common root rot, caused by Bipolaris sorokiniana, is one of the most prevalent diseases of wheat and has led to major declines in wheat yield and quality worldwide. Here, strain XZ34-1 was isolated from soil and identified as Bacillus amyloliquefaciens based on the morphological, physiological, biochemical characteristics and 16S rDNA sequence. Culture filtrate (CF) of strain XZ34-1 showed a high inhibition rate against B.sorokiniana and had a broad antifungal spectrum. It also remarkably inhibited the mycelial growth and spore germination of B. sorokiniana. In pot control experiments, the incidence and disease index of common root rot in wheat seedlings were decreased after treatment with CF, and the biological control efficacy was significant, up to 78.24%. Further studies showed XZ34-1 could produce antifungal bioactive substances and had the potential of promoting plant growth. Lipopeptide genes detection with PCR indicated that strain XZ34-1 may produce lipopeptides. Furthermore, activities of defense-related enzymes were enhanced in wheat seedlings after inoculation with B.sorokiniana and treatment with CF, which showed induced resistance could be produced in wheat to resist pathogens. These results reveal that strain XZ34-1 is a promising candidate for application as a biological control agent against B.sorokiniana.


Plant Disease ◽  
2021 ◽  
Author(s):  
Hafiz Muhammad Usman Aslam ◽  
Nasir Ahmad Khan ◽  
Syed Ismat Hussain ◽  
Yasir Ali ◽  
Muhammad Raheel ◽  
...  

Brown leaf spot of rice is one of the major seed-borne diseases and can diminish grain production up to 52% (Barnwal et al. 2013). In 2018, infected leaf samples showing the typical symptoms of brown spots were collected from the vicinity of the University of Agriculture, Faisalabad (31°26'10.3"N 73°03'35.1"E). The symptoms were brown-dark spots, with gray-light gray or brown centers surrounded by dark margins and with chlorotic halos and of oval or cylindrical shapes (5 to 9 mm in diameter). Disease incidence averaged 61% across the seven fields observed. Leaves were collected from the seven infected fields and symptomatic leaf tissues of 5 mm2 were excised from representative necrotic spots in each. These tissues were surface disinfected with 70% ethanol, rinsed with sterile distilled water (SDW), dried by blotting on paper, and placed on potato dextrose agar medium. For pathogen growth, the plates were placed at 25oC (±2oC) with a 12-hour photoperiod for 5 days. Five samples from each of the infected fields were taken for pathogen isolation and among them ten isolates were sub-cultured and purified by using the single spore method. The resulting fungal colonies were fluffy and ranged in color from grayish black/black to light brown. Fifteen conidia were measured that are olivaceous-brown to dark brown in color, elliptical to oblong with narrow (tapered) ends, with 3-10 septa and 35.6-65.4 µm in length x 13.1-25.7 µm in width. Conidiophores were yellowish-brown, geniculate, and solitary (Pratt 2003). For molecular studies, rDNA of the internal transcribed spacer (ITS) region, translation elongation factor (tef), RNA polymerase II second largest subunit (rpb2) and glyceraldehyde-3-phosphate dehydrogenase (gpd) gene were amplified by using the primers ITS1F/ITS4R (White et al. 1990), EF1-983F/EF1-2218R (Rehner and Buckley 2005), 5F2/7CR (O’Donnell et al. 2007), and GPD1/GPD2 (Berbee et al. 1999) respectively. The sequence of all the amplified gene regions of one SUL-1 isolate was deposited into GenBank with accession numbers MN314844 (ITS), MN326866 (tef), MN990457 (rpb2) and MN990456 (gpd). BLASTn queries of the obtained sequences (ITS, tef, rpb2 and gpd) showed 99-100% homology with the corresponding nucleotide sequences of B. sorokiniana (GenBank accession nos. GU480767, MF490855, LT715652 and MK558818 respectively). To fulfill the Koch’s postulates, twenty rice plants (cv. Basmati-385) were sprayed at 2 to 3 leaf stages by using the two representative isolates with a spore suspension of 105 spores/ml. SDW was sprayed on ten control plants. The plants were covered with polyethylene bags to keep the moisture contents and incubated at 25oC (±2oC) for 7 days. After a week, same symptoms as those described above were observed. In the repeated experiment, B. sorokiniana was re-isolated from the infected rice leaves and confirmed morphologically; fulfill the Koch’s postulates. With grave worry, the other species of the genus Bipolaris (B. oryzae, and B. victoriae) have also been found to the cause brown leaf spot of rice (Motlagh and Kaviani 2008). To our knowledge, this is the first report of Bipolaris sorokiniana causing brown leaf spot of rice in Pakistan. Because rice is highly consumable grain in Pakistan, so the rapid spread of this disease in the rice farming areas is of a serious concern.


2021 ◽  
Vol 9 ◽  
Author(s):  
Yuqi Cui ◽  
Yinuo Wang ◽  
Yuwen Zhang ◽  
Liangyue Pang ◽  
Yan Zhou ◽  
...  

Purpose: Severe early childhood caries (S-ECC) is a microbe-mediated disease with tooth hard tissue destruction. However, the role of the fungal community in various ecological niches of deciduous dental caries has not been fully elucidated. This study aimed to analyze the differences of mycobiome in diverse spatial niches with and without S-ECC.Method: A total of 48 samples were obtained from 8 S-ECC children (SE group) and 8 caries-free children (CF group) aged 4–5 years. Unstimulated saliva (S), healthy supragingival plaque (FMIX), mixed plaque from decayed teeth (SMIX) and carious lesion (DMIX) samples were collected. The ITS2 region of the fungi was amplified and sequenced using the Ion S5™XL platform.Results: A total of 281 species were identified. Candida albicans showed relatively higher abundance in S-ECC children, while Alternaria alternata and Bipolaris sorokiniana were more enriched in CF group. In this study, the relative abundance of C. albicans in CF.FMIX (0.4%), SE.FMIX (12.5%), SE.SMIX (24.0%), and SE.DMIX (37.2%) increased successively. Significant differences of fungal species richness and diversity were observed between SE.FMIX-SE.SMIX, SE.FMIX-SE.DMIX (P < 0.05).Conclusions: The diversity of fungal communities in S-ECC children showed significant differences in various spatial niches of primary teeth. The richness of C. albicans was closely related to the caries states and depth, suggesting that it may play a crucial role in caries pathogenicity.


Author(s):  
Kartar Singh ◽  
Rashmi Aggarwal ◽  
Praveen Kumar Verma ◽  
Sandhya Verma ◽  
Sapna Sharma ◽  
...  

2021 ◽  
Vol 2064 (1) ◽  
pp. 012101
Author(s):  
N N Loy ◽  
N I Sanzharova ◽  
S N Gulina ◽  
O V Suslova ◽  
T V Chizh ◽  
...  

Abstract The effect of electron irradiation of barley seeds of the Vladimir variety on the development of plants, disease incidence and productivity in controlled greenhouse conditions has been studied. It was found that the effectiveness of the effect of seed treatment with low-energy electronic radiation in the dose range of 30–150 kGy on the morphometric parameters of barley largely depends on the accelerating voltage – 130 kV (mode 1) and 160 kV (mode 2). It was noted that irradiation suppressed the formation of chlorophyll a and b and carotenoids in barley leaves at all doses and irradiation regimes and, at the tendency level, reduced the infestation of Bipolaris sorokiniana plants in the tillering and heading phases. It was shown that irradiation of seeds caused an increase in the total tillering of plants (mode 2), but at the same time reduced the productive tillering. Electron irradiation caused an increase in the mass of 1000 grains and did not affect the yield of grain and straw.


2021 ◽  
Author(s):  
Qiaoyun Li ◽  
Runyu Hu ◽  
Zhenfeng Guo ◽  
Siyu Wang ◽  
Chuang Gao ◽  
...  

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