Isolation of Viral-Infected Brain Regions for miRNA Profiling from Formalin-Fixed Paraffin-Embedded Tissues by Laser Capture Microdissection

2018 ◽  
pp. 41-51
Author(s):  
Anna Majer ◽  
Stephanie A. Booth
Keyword(s):  
Laser Capture Microdissection ◽  
Brain Regions ◽  
Mirna Profiling ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Laser Capture ◽  
Formalin Fixed

Improved Detection ofHelicobacter pyloriDNA in Formalin-fixed Paraffin-embedded (FFPE) Tissue of Patients with Hepatocellular Carcinoma Using Laser Capture Microdissection (LCM)

Helicobacter ◽  
2013 ◽  
Vol 18 (3) ◽  
pp. 244-245 ◽  
Author(s):  
Elizabeth Maria Afonso Rabelo-Gonçalves ◽  
Ilária C. Sgardioli ◽  
Iscia Lopes-Cendes ◽  
Cecília Amélia Fazzio Escanhoela ◽  
Jazon Romilson de Souza Almeida ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Laser Capture Microdissection ◽  
Ffpe Tissue ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Laser Capture ◽  
Formalin Fixed

scholarly journals Approaching tumour heterogeneity using laser capture microdissection coupled with gene expression on formalin-fixed paraffin canine mammary tumours

2018 ◽  
pp. 567-567
Author(s):  
Silvia Guil-Luna ◽  
Francisco Javier Salguero ◽  
Jaime Gómez-Laguna ◽  
Abdullah Alijojani ◽  
Waldo García-Jimenez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Laser Capture Microdissection ◽  
Tumour Heterogeneity ◽  
Mammary Tumours ◽  
Formalin Fixed Paraffin ◽  
Laser Capture ◽  
Formalin Fixed

scholarly journals Laser capture microdissection coupled mass spectrometry (LCM-MS) for spatially resolved analysis of formalin-fixed and stained human lung tissues

2019 ◽  
Author(s):  
Jeremy A. Herrera ◽  
Venkatesh Mallikarjun ◽  
Silvia Rosini ◽  
Maria Angeles Montero ◽  
Stacey Warwood ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Capture Microdissection ◽  
Human Lung ◽  
Heat Extraction ◽  
Preparation Methods ◽  
Spatially Resolved ◽  
Formalin Fixed Paraffin Embedded ◽  
Ffpe Tissues ◽  
Laser Capture ◽  
Formalin Fixed

ABSTRACTHaematoxylin and eosin (H&E) – which respectively stain nuclei blue and other cellular and stromal material pink – are routinely used for clinical diagnosis based on the identification of morphological features. A richer characterization can be achieved by laser capture microdissection coupled to mass spectrometry (LCM-MS), giving an unbiased assay of the proteins that make up the tissue. However, the process of fixing, and H&E staining of tissues is poorly compatible with standard sample preparation methods for mass spectrometry, resulting in low protein yield. Here we describe a microproteomics technique optimized to analyze H&E-stained, formalin-fixed paraffin-embedded (FFPE) tissues. We advance our methodology by combining 3 techniques shown to individually enhance protein yields (heat extraction, physical disruption, and in column digestion) into one optimized pipeline for the analysis of H&E stained FFPE tissues. Micro-dissected morphologically normal human lung alveoli (0.082 mm3) and human lung blood vessels (0.094 mm3) from FFPE fixed section from Idiopathic Pulmonary Fibrosis (IPF) specimens were then subject to comparative proteomics using this methodology. This approach yielded 1252 differentially expressed proteins including 137 extracellular matrix (ECM) proteins. In addition, we offer proof of principal that MS can identify distinct, characteristic proteomic compositions of anatomical features within complex tissues.

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