rna sequencing
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2022 ◽  
Vol 22 (2) ◽  
pp. 100577
Author(s):  
Amr Elshalofy ◽  
Karen Wagener ◽  
Katharina Weber ◽  
Miguel Blanco ◽  
Stefan Bauersachs ◽  
...  
Keyword(s):  

2022 ◽  
Vol 3 (1) ◽  
pp. 101064
Author(s):  
Andrew G. Shuparski ◽  
Brett W. Higgins ◽  
Karen B. Miller ◽  
Chad R. Dufaud ◽  
Amanda M. Robinson ◽  
...  

2022 ◽  
Vol 3 (1) ◽  
pp. 101036
Author(s):  
Adelina Rabenius ◽  
Sajitha Chandrakumaran ◽  
Lea Sistonen ◽  
Anniina Vihervaara

2022 ◽  
Vol 42 (3) ◽  
pp. 380-383
Author(s):  
Rae Na ◽  
Jinyoung Hong ◽  
Hyunjung Gu ◽  
Woochang Lee ◽  
Jae-Lyun Lee ◽  
...  

2022 ◽  
Vol 3 (1) ◽  
pp. 101041
Author(s):  
Saran Pankaew ◽  
Clémence Grosjean ◽  
Julie Quessada ◽  
Marie Loosveld ◽  
Delphine Potier ◽  
...  

2022 ◽  
Vol 146 ◽  
pp. 112558
Author(s):  
Nafiseh Erfanian ◽  
Afshin Derakhshani ◽  
Saeed Nasseri ◽  
Mohammad Fereidouni ◽  
Behzad Baradaran ◽  
...  

2022 ◽  
Vol 12 (2) ◽  
pp. 346-351
Author(s):  
Dong-Yang Guo ◽  
Zhou-Xin Yang ◽  
Guo-Long Cai ◽  
Ling-Zhi Shen ◽  
Ying-Xing Yue ◽  
...  

Background: Lipopolysaccharide (LPS) desensitization, which is characterized by hyporesponsiveness and a form of immunosuppression, is important in the negative regulation of responses to LPS and inflammatory disease such as sepsis. However, effect of IL-33 in the desensitization to LPS remains unclear. Methods: We used RNA-sequencing technology to analyze changes in mRNA in bone-marrow-derived macrophages (BMDMs) stimulated with LPS. Changes in expression and secretion of inflammatory cytokines were detected by qPCR and ELISA, respectively. Mechanisms were further studied through p65 phosphorylation detection. Results: IL-33 expression was significantly increased in LPS-treated macrophages, indicating its involvement in LPS-induced inflammation. Exogenous IL-33 increased the inflammatory response and ameliorated LPS desensitization by increasing the secretion of proinflammatory cytokines. It also activated p65 phosphorylation in resistant cells. Conclusion: IL-33 can enhance the inflammatory response induced by LPS and ameliorate LPS desensitization possibly by activating the NF-κB pathway in mouse macrophages.


Cells ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 300
Author(s):  
Hiroshi Ohguro ◽  
Yosuke Ida ◽  
Fumihito Hikage ◽  
Araya Umetsu ◽  
Hanae Ichioka ◽  
...  

To elucidate the currently unknown mechanisms responsible for the diverse biological aspects between two-dimensional (2D) and three-dimensional (3D) cultured 3T3-L1 preadipocytes, RNA-sequencing analyses were performed. During a 7-day culture period, 2D- and 3D-cultured 3T3-L1 cells were subjected to lipid staining by BODIPY, qPCR for adipogenesis related genes, including peroxisome proliferator-activated receptor γ (Pparγ), CCAAT/enhancer-binding protein alpha (Cebpa), Ap2 (fatty acid-binding protein 4; Fabp4), leptin, and AdipoQ (adiponectin), and RNA-sequencing analysis. Differentially expressed genes (DEGs) were detected by next-generation RNA sequencing (RNA-seq) and validated by a quantitative reverse transcription–polymerase chain reaction (qRT–PCR). Bioinformatic analyses were performed on DEGs using a Gene Ontology (GO) enrichment analysis and an Ingenuity Pathway Analysis (IPA). Significant spontaneous adipogenesis was observed in 3D 3T3-L1 spheroids, but not in 2D-cultured cells. The mRNA expression of Pparγ, Cebpa, and Ap2 among the five genes tested were significantly higher in 3D spheroids than in 2D-cultured cells, thus providing support for this conclusion. RNA analysis demonstrated that a total of 826 upregulated and 725 downregulated genes were identified as DEGs. GO enrichment analysis and IPA found 50 possible upstream regulators, and among these, 6 regulators—transforming growth factor β1 (TGFβ1), signal transducer and activator of transcription 3 (STAT3), interleukin 6 (IL6), angiotensinogen (AGT), FOS, and MYC—were, in fact, significantly upregulated. Further analyses of these regulators by causal networks of the top 14 predicted diseases and functions networks (IPA network score indicated more than 30), suggesting that STAT3 was the most critical upstream regulator. The findings presented herein suggest that STAT3 has a critical role in regulating the unique biological properties of 3D spheroids that are produced from 3T3-L1 preadipocytes.


2022 ◽  
Vol 19 (1) ◽  
Author(s):  
Joachim R. de Miranda ◽  
Laura E. Brettell ◽  
Nor Chejanovsky ◽  
Anna K. Childers ◽  
Anne Dalmon ◽  
...  

AbstractIn 1977, a sample of diseased adult honeybees (Apis mellifera) from Egypt was found to contain large amounts of a previously unknown virus, Egypt bee virus, which was subsequently shown to be serologically related to deformed wing virus (DWV). By sequencing the original isolate, we demonstrate that Egypt bee virus is in fact a fourth unique, major variant of DWV (DWV-D): more closely related to DWV-C than to either DWV-A or DWV-B. DWV-A and DWV-B are the most common DWV variants worldwide due to their close relationship and transmission by Varroa destructor. However, we could not find any trace of DWV-D in several hundred RNA sequencing libraries from a worldwide selection of honeybee, varroa and bumblebee samples. This means that DWV-D has either become extinct, been replaced by other DWV variants better adapted to varroa-mediated transmission, or persists only in a narrow geographic or host range, isolated from common bee and beekeeping trade routes.


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