Epitope Mapping of Antibodies Using Bacterial Cell Surface Display of Gene Fragment Libraries

2018 ◽  
pp. 141-157
Author(s):  
Anna-Luisa Volk ◽  
Francis Jingxin Hu ◽  
Johan Rockberg
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Epitope Mapping ◽  
Surface Display ◽  
Cell Surface Display ◽  
Gene Fragment ◽  
Bacterial Cell Surface ◽  
Fragment Libraries

scholarly journals Bacterial cell surface display for epitope mapping of hepatitis C virus core antigen

2003 ◽  
Vol 226 (2) ◽  
pp. 347-353 ◽  
Author(s):  
Su-Min Kang ◽  
Jin-Kyu Rhee ◽  
Eui-Joong Kim ◽  
Kwang-Hyub Han ◽  
Jong-Won Oh
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Surface ◽  
Bacterial Cell ◽  
Epitope Mapping ◽  
Surface Display ◽  
Cell Surface Display ◽  
Core Antigen ◽  
Bacterial Cell Surface ◽  
Virus Core

scholarly journals Cover Feature: Bacterial Cell‐Surface Display of Semisynthetic Cyclic Peptides (ChemBioChem 1/2019)

ChemBioChem ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 4-4
Author(s):  
Shubhendu Palei ◽  
Kira S. Becher ◽  
Christian Nienberg ◽  
Joachim Jose ◽  
Henning D. Mootz
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cyclic Peptides ◽  
Surface Display ◽  
Cell Surface Display ◽  
Bacterial Cell Surface

Bacterial Cell Surface Display of a Multifunctional Cellulolytic Enzyme Screened from a Bovine Rumen Metagenomic Resource

2015 ◽  
Vol 25 (11) ◽  
pp. 1835-1841 ◽  
Author(s):  
Kyong-Cheol Ko ◽  
Binna Lee ◽  
Dae-Eun Cheong ◽  
Yunjon Han ◽  
Jong Hyun Choi ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Surface Display ◽  
Cell Surface Display ◽  
Cellulolytic Enzyme ◽  
Bovine Rumen ◽  
Bacterial Cell Surface

Evaluation of a Temperature-Induced Chitosanase Bacterial Cell Surface Display System for the Efficient Production of Chitooligosaccharides

2021 ◽  
Author(s):  
Qianqian Li ◽  
Tuantuan Wang ◽  
Yangzhi Ye ◽  
Shimin Guan ◽  
Baoguo Cai ◽  
...  
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Surface Display ◽  
Cell Surface Display ◽  
Efficient Production ◽  
Display System ◽  
Ice Nucleation Protein ◽  
Bacterial Cell Surface ◽  
Hydrolysis Temperature ◽  
Cell Surface Display System

Abstract Objective To establish a temperature-induced chitosanase bacterial cell surface display system to produce chitooligosaccharides (COSs) efficiently for industrial applications. Results Temperature-inducible chitosanase CSN46A bacterial surface display systems containing one or two copies of ice nucleation protein (InaQ-N) as anchoring motifs were successfully constructed on the basis of Escherichia coli and named as InaQ-N-CSN46A and 2InaQ-N-CSN46A. The specific enzyme activity of 2InaQ-N-CSN46A reached 886.33±0.81 U/g cell dry weight, which was 45.6% higher than that of InaQ-N-CSN46A. However, few proteins were detected in 2InaQ-N-CSN46A hydrolysis system. Therefore, 2InaQ-N-CSN46A had higher hydrolysis efficiency and stability than InaQ-N-CSN46A. GPC revealed that under the optimum enzymatic hydrolysis temperature, the final products were mainly chitobiose and chitotriose. Chitopentaose accumulated (77.62%) when the hydrolysis temperature reached 60 ℃. FTIR and NMR analysis demonstrated that the structures of the two hydrolysis products were consistent with those of COSs.Conclusions In this study, chitosanase was expressed on the surfaces of E. coli by increasing induction temperature, and chitosan was hydrolysed directly without enzyme purification steps. This study provided a novel strategy for industrial COSs production.

Bacterial Cell‐Surface Display of Semisynthetic Cyclic Peptides

ChemBioChem ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 72-77 ◽  
Author(s):  
Shubhendu Palei ◽  
Kira S. Becher ◽  
Christian Nienberg ◽  
Joachim Jose ◽  
Henning D. Mootz
Keyword(s):  
Cell Surface ◽  
Bacterial Cell ◽  
Cyclic Peptides ◽  
Surface Display ◽  
Cell Surface Display ◽  
Bacterial Cell Surface
Sign in / Sign up

Export Citation Format

Share Document