T-DNA Insertional Mutagenesis and Activation Tagging in Medicago truncatula

2018 ◽  
pp. 83-105
Author(s):  
Francesco Panara ◽  
Ornella Calderini ◽  
Andrea Porceddu
Keyword(s):  
Medicago Truncatula ◽  
Insertional Mutagenesis ◽  
Activation Tagging ◽  
Dna Insertional Mutagenesis

scholarly journals T-DNA Insertional Mutagenesis for Activation Tagging in Rice

2002 ◽  
Vol 130 (4) ◽  
pp. 1636-1644 ◽  
Author(s):  
Dong-Hoon Jeong ◽  
Suyoung An ◽  
Hong-Gyu Kang ◽  
Sunok Moon ◽  
Jong-Jin Han ◽  
...  
Keyword(s):  
Insertional Mutagenesis ◽  
Activation Tagging ◽  
Dna Insertional Mutagenesis

scholarly journals Embryonic Lethals and T-DNA Insertional Mutagenesis in Arabidopsis

1991 ◽  
Vol 3 (2) ◽  
pp. 149 ◽  
Author(s):  
Deena Errampalli ◽  
David Patton ◽  
Linda Castle ◽  
Leigh Mickelson ◽  
Karl Hansen ◽  
...  
Keyword(s):  
Insertional Mutagenesis ◽  
Dna Insertional Mutagenesis

scholarly journals Chlamydomonas WDR92 in association with R2TP-like complex and multiple DNAAFs to regulate ciliary dynein preassembly

2018 ◽  
Vol 11 (9) ◽  
pp. 770-780 ◽  
Author(s):  
Guang Liu ◽  
Limei Wang ◽  
Junmin Pan
Keyword(s):  
Mass Spectrometry ◽  
Insertional Mutagenesis ◽  
The Other ◽  
Cytoplasmic Protein ◽  
Flagellar Motility ◽  
Heavy Chains ◽  
The Third ◽  
Axonemal Dynein ◽  
Dynein Arms ◽  
Dna Insertional Mutagenesis

Abstract The motility of cilia or eukaryotic flagella is powered by the axonemal dyneins, which are preassembled in the cytoplasm by proteins termed dynein arm assembly factors (DNAAFs) before being transported to and assembled on the ciliary axoneme. Here, we characterize the function of WDR92 in Chlamydomonas. Loss of WDR92, a cytoplasmic protein, in a mutant wdr92 generated by DNA insertional mutagenesis resulted in aflagellate cells or cells with stumpy or short flagella, disappearance of axonemal dynein arms, and diminishment of dynein arm heavy chains in the cytoplasm, suggesting that WDR92 is a DNAAF. Immunoprecipitation of WDR92 followed by mass spectrometry identified inner dynein arm heavy chains and multiple DNAAFs including RuvBL1, RPAP3, MOT48, ODA7, and DYX1C. The PIH1 domain-containing protein MOT48 formed a R2TP-like complex with RuvBL1/2 and RPAP3, while PF13, another PIH1 domain-containing protein with function in dynein preassembly, did not. Interestingly, the third PIH1 domain-containing protein TWI1 was not related to flagellar motility. WDR92 physically interacted with the R2TP-like complex and the other identified DNNAFs. Our data suggest that WDR92 functions in association with the HSP90 co-chaperone R2TP-like complex as well as linking other DNAAFs in dynein preassembly.

Diverse developmental mutants revealed in an activation-tagged population of poplarThis article is one of a selection of papers published on the Special Issue of Poplar Research in Canada.

2007 ◽  
Vol 85 (11) ◽  
pp. 1071-1081 ◽  
Author(s):  
Edward J. Harrison ◽  
Michael Bush ◽  
Jonathan M. Plett ◽  
Daniel P. McPhee ◽  
Robin Vitez ◽  
...  
Keyword(s):  
Large Scale ◽  
Insertional Mutagenesis ◽  
Hybrid Poplar ◽  
Activation Tagging ◽  
Developmental Abnormalities ◽  
Endogenous Gene ◽  
Developmental Mutants ◽  
Poplar Trees ◽  
Wide Range ◽  
Mutant Lines

We have produced the largest population of activation-tagged poplar trees to date, approximately 1800 independent lines, and report on phenotypes of interest that have been identified in tissue culture and greenhouse conditions. Activation tagging is an insertional mutagenesis technique that results in the dominant upregulation of an endogenous gene. A large-scale Agrobacterium -mediated transformation protocol was used to transform the pSKI074 activation-tagging vector into Populus tremula × Populus alba hybrid poplar. We have screened the first 1000 lines for developmental abnormalities and have a visible mutant frequency of 2.4%, with alterations in leaf and stem structure as well as overall stature. Most of the phenotypes represent new phenotypes that have not previously been identified in poplar and, in some cases, not in any other plant either. Molecular analysis of the T-DNA inserts of a subpopulation of mutant lines reveal both single and double T-DNA inserts with double inserts more common in lines with visible phenotypes. The broad range of developmental mutants identified in this pilot screen of the population reveals that it will be a valuable resource for gene discovery in poplar. The full value of this population will only be realized as we screen these lines for a wide range of phenotypes.

Insertional mutagenesis: a Swiss Army knife for functional genomics of Medicago truncatula

2005 ◽  
Vol 10 (5) ◽  
pp. 229-235 ◽  
Author(s):  
Million Tadege ◽  
Pascal Ratet ◽  
Kirankumar S. Mysore
Keyword(s):  
Functional Genomics ◽  
Medicago Truncatula ◽  
Insertional Mutagenesis

T-DNA insertional mutagenesis in Arabidopsis

1992 ◽  
Vol 20 (5) ◽  
pp. 963-976 ◽  
Author(s):  
Csaba Koncz ◽  
Kinga N�meth ◽  
George P. R�dei ◽  
Jeff Schell
Keyword(s):  
Insertional Mutagenesis ◽  
Dna Insertional Mutagenesis

T-DNA insertional mutagenesis and promoter trapping in Lycopersicon esculentum

2008 ◽  
Vol 136 ◽  
pp. S229
Author(s):  
Ismanizan Ismail ◽  
Siti Izera Ismail ◽  
Zamri Zainal
Keyword(s):  
Lycopersicon Esculentum ◽  
Insertional Mutagenesis ◽  
Promoter Trapping ◽  
Dna Insertional Mutagenesis

Genetic Dissection of T-DNA Insertional Mutants Reveals Uncoupling of Dikaryotic Filamentation and Virulence in Sugarcane Smut Fungus

2021 ◽  
Author(s):  
Shan Lu ◽  
Feng Guo ◽  
Zhiqiang Wang ◽  
Xiaorui Shen ◽  
Yizhen Deng ◽  
...  
Keyword(s):  
Regulatory Networks ◽  
Insertional Mutagenesis ◽  
Genetic Regulation ◽  
Genetic Dissection ◽  
Opposite Mating Type ◽  
Basidiomycetous Fungus ◽  
Sporisorium Scitamineum ◽  
Type Strains ◽  
Dna Insertional Mutagenesis ◽  
Teliospore Development

The biotrophic basidiomycetous fungus Sporisorium scitamineum causing smut disease in sugarcane is characterized by a life-cycle composed of a yeast-like nonpathogenic haploid basidiosporial stage outside the plant and filamentous pathogenic dikaryotic hyphae within the plant. Under field conditions, dikaryotic hyphae are formed after mating of two opposite mating-type strains. However, the mechanisms underlying genetic regulation of filamentation and its association with pathogenicity and development of teliospores are currently unclear. This study has focused on the characterization and genetic dissection of haploid filamentous mutants derived from T-DNA insertional mutagenesis. Our results support the existence of at least three genotypes among the six haploid filamentous mutants that differentially contribute to virulence and development of the whip and teliospore, providing a novel foundation for further investigation of the regulatory networks associated with pathogenicity and teliospore development in S. scitamineum.

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