Activation Tagging and Insertional Mutagenesis in Barley

2010 ◽  
pp. 107-128 ◽  
Author(s):  
Michael A. Ayliffe ◽  
Anthony J. Pryor
Keyword(s):  
Insertional Mutagenesis ◽  
Activation Tagging

Diverse developmental mutants revealed in an activation-tagged population of poplarThis article is one of a selection of papers published on the Special Issue of Poplar Research in Canada.

2007 ◽  
Vol 85 (11) ◽  
pp. 1071-1081 ◽  
Author(s):  
Edward J. Harrison ◽  
Michael Bush ◽  
Jonathan M. Plett ◽  
Daniel P. McPhee ◽  
Robin Vitez ◽  
...  
Keyword(s):  
Large Scale ◽  
Insertional Mutagenesis ◽  
Hybrid Poplar ◽  
Activation Tagging ◽  
Developmental Abnormalities ◽  
Endogenous Gene ◽  
Developmental Mutants ◽  
Poplar Trees ◽  
Wide Range ◽  
Mutant Lines

We have produced the largest population of activation-tagged poplar trees to date, approximately 1800 independent lines, and report on phenotypes of interest that have been identified in tissue culture and greenhouse conditions. Activation tagging is an insertional mutagenesis technique that results in the dominant upregulation of an endogenous gene. A large-scale Agrobacterium -mediated transformation protocol was used to transform the pSKI074 activation-tagging vector into Populus tremula × Populus alba hybrid poplar. We have screened the first 1000 lines for developmental abnormalities and have a visible mutant frequency of 2.4%, with alterations in leaf and stem structure as well as overall stature. Most of the phenotypes represent new phenotypes that have not previously been identified in poplar and, in some cases, not in any other plant either. Molecular analysis of the T-DNA inserts of a subpopulation of mutant lines reveal both single and double T-DNA inserts with double inserts more common in lines with visible phenotypes. The broad range of developmental mutants identified in this pilot screen of the population reveals that it will be a valuable resource for gene discovery in poplar. The full value of this population will only be realized as we screen these lines for a wide range of phenotypes.

scholarly journals T-DNA Insertional Mutagenesis for Activation Tagging in Rice

2002 ◽  
Vol 130 (4) ◽  
pp. 1636-1644 ◽  
Author(s):  
Dong-Hoon Jeong ◽  
Suyoung An ◽  
Hong-Gyu Kang ◽  
Sunok Moon ◽  
Jong-Jin Han ◽  
...  
Keyword(s):  
Insertional Mutagenesis ◽  
Activation Tagging ◽  
Dna Insertional Mutagenesis

Constructing and Phenotypic Analyzing an Activation-Tagging Arabidopsis Mutant Pool

2010 ◽  
Vol 32 (1) ◽  
pp. 53-59
Author(s):  
Yan-Long GUAN ◽  
Wan-Sha LI ◽  
Kui-De YIN ◽  
Yong-Ping YANG ◽  
Xiang-Yang HU
Keyword(s):  
Activation Tagging ◽  
Mutant Pool

Insertional Mutagenesis of Chlamydomonas reinhardtii

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (24) ◽  
Author(s):  
Chia-Hong Tsai ◽  
Christoph Benning
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Insertional Mutagenesis

scholarly journals High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica

2021 ◽  
Author(s):  
Christian Südfeld ◽  
Michal Hubáček ◽  
Daniel Rodrigues Figueiredo ◽  
Mihris I.S. Naduthodi ◽  
John van der Oost ◽  
...  
Keyword(s):  
High Throughput ◽  
Lipid Accumulation ◽  
Insertional Mutagenesis ◽  
Nannochloropsis Oceanica ◽  
Novel Targets

scholarly journals Virus-Driven Carcinogenesis

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2625
Author(s):  
Yuichiro Hatano ◽  
Takayasu Ideta ◽  
Akihiro Hirata ◽  
Kayoko Hatano ◽  
Hiroyuki Tomita ◽  
...  
Keyword(s):  
Insertional Mutagenesis ◽  
Human Herpesvirus ◽  
Kaposi Sarcoma ◽  
Malignant Cell ◽  
Precision Oncology ◽  
Oncogenic Viruses ◽  
Barr Virus ◽  
Human T Cell ◽  
Tumor Viruses ◽  
Tumor Virus

Cancer arises from the accumulation of genetic and epigenetic alterations. Even in the era of precision oncology, carcinogens contributing to neoplastic process are still an important focus of research. Comprehensive genomic analyses have revealed various combinations of base substitutions, referred to as the mutational signatures, in cancer. Each mutational signature is believed to arise from specific DNA damage and repair processes, including carcinogens. However, as a type of carcinogen, tumor viruses increase the cancer risk by alternative mechanisms, including insertional mutagenesis, viral oncogenes, and immunosuppression. In this review, we summarize virus-driven carcinogenesis to provide a framework for the control of malignant cell proliferation. We first provide a brief overview of oncogenic viruses and describe their implication in virus-related tumors. Next, we describe tumor viruses (HPV, Human papilloma virus; HBV, Hepatitis B virus; HCV, Hepatitis C virus; EBV, Epstein–Barr virus; Kaposi sarcoma herpesvirus; MCV, Merkel cell polyoma virus; HTLV-1, Human T-cell lymphotropic virus, type-1) and tumor virus-related cancers. Lastly, we introduce emerging tumor virus candidates, human cytomegalovirus (CMV), human herpesvirus-6 (HHV-6) and adeno-associated virus-2 (AAV-2). We expect this review to be a hub in a complex network of data for virus-associated carcinogenesis.

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