Bacterial Expression of Membrane-Associated Cytochrome P450s and Their Activity Assay in Nanodiscs

Methods in Molecular Biology - Microbial Metabolic Engineering ◽

10.1007/978-1-4939-9142-6_5 ◽

2019 ◽

pp. 47-72

Author(s):

Demetrios Maroutsos ◽

Hannah Huff ◽

Aditi Das

Keyword(s):

Cytochrome P450s ◽

Bacterial Expression ◽

Activity Assay

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Enhanced bacterial expression of several mammalian cytochrome P450s by codon optimization and chaperone coexpression

Biotechnology Letters ◽

10.1007/s10529-009-0059-5 ◽

2009 ◽

Vol 31 (10) ◽

pp. 1589-1593 ◽

Cited By ~ 20

Author(s):

Zhong-Liu Wu ◽

Jing Qiao ◽

Zhi-Gang Zhang ◽

F. Peter Guengerich ◽

Yan Liu ◽

...

Keyword(s):

Codon Optimization ◽

Cytochrome P450s ◽

Bacterial Expression

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Azocoll protease activity assay

Protocol Exchange ◽

10.1038/nprot.2007.484 ◽

2007 ◽

Cited By ~ 4

Author(s):

Naxin Jiang ◽

Nguan Soon Tan ◽

Bow Ho ◽

Jeak Ling Ding

Keyword(s):

Protease Activity ◽

Activity Assay

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Development of a collagen-binding activity assay as a screening test for type II von Willebrand disease in dogs

Journal of the American Veterinary Medical Association ◽

10.2460/javma.228.4.567 ◽

2006 ◽

Vol 228 (4) ◽

pp. 567-567

Author(s):

Elizabeth Peet Sabino ◽

Hollis N. Erb ◽

James L. Catalfamo

Keyword(s):

Screening Test ◽

Binding Activity ◽

Von Willebrand Disease ◽

Type Ii ◽

Activity Assay ◽

Collagen Binding ◽

Von Willebrand

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Faculty Opinions recommendation of (+)-(10R)-Germacrene A synthase from goldenrod, Solidago canadensis; cDNA isolation, bacterial expression and functional analysis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1009311.122859 ◽

2002 ◽

Author(s):

John W Mansfield

Keyword(s):

Functional Analysis ◽

Bacterial Expression ◽

Solidago Canadensis ◽

Cdna Isolation

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Faculty Opinions recommendation of Bacterial expression and purification of active hematopoietic cell kinase.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.10734957.11627055 ◽

2011 ◽

Author(s):

Mario Lebendiker

Keyword(s):

Bacterial Expression ◽

Hematopoietic Cell ◽

Expression And Purification ◽

Hematopoietic Cell Kinase

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Faculty Opinions recommendation of A site-specific, multiplexed kinase activity assay using stable-isotope dilution and high-resolution mass spectrometry.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1161908.623533 ◽

2009 ◽

Author(s):

Jonathan Chernoff

Keyword(s):

Mass Spectrometry ◽

High Resolution ◽

Kinase Activity ◽

High Resolution Mass Spectrometry ◽

Activity Assay ◽

Site Specific ◽

Stable Isotope Dilution ◽

A Site ◽

Kinase Activity Assay ◽

Resolution Mass

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Faculty Opinions recommendation of Non-natural olefin cyclopropanation catalyzed by diverse cytochrome P450s and other hemoproteins.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.720329823.793515627 ◽

2016 ◽

Author(s):

Gregory Stephanopoulos

Keyword(s):

Cytochrome P450s

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Expression and Enzyme Activity Assay of pfu with Molecular Chaperone

Acta Botanica Yunnanica ◽

10.3724/sp.j.1143.2009.00499 ◽

2010 ◽

Vol 31 (6) ◽

pp. 499-503

Author(s):

Hai-Jun ZHANG ◽

Jun YANG ◽

Xiao-Guang LIU ◽

Xiang-Yang HU

Keyword(s):

Enzyme Activity ◽

Molecular Chaperone ◽

Activity Assay ◽

Enzyme Activity Assay

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Catalase Activity Assay in Candida glabrata

BIO-PROTOCOL ◽

10.21769/bioprotoc.1072 ◽

2014 ◽

Vol 4 (6) ◽

Cited By ~ 5

Author(s):

Emmanuel Orta-Zavalza ◽

Marcela Briones-Martin-del-Campo ◽

Irene Castano ◽

Alejandro Penas

Keyword(s):

Catalase Activity ◽

Candida Glabrata ◽

Activity Assay

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Expression, Purification and in vitro Enzyme Activity Assay of Plant Derived GTPase

BIO-PROTOCOL ◽

10.21769/bioprotoc.1651 ◽

2015 ◽

Vol 5 (22) ◽

Author(s):

Annemarie Gl�ckner ◽

Christian Voigt

Keyword(s):

Enzyme Activity ◽

Activity Assay ◽

Enzyme Activity Assay

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