Catalase Activity Assay in Candida glabrata

We have found that microbodies appeared profusely together with a remarkable increase in catalase activity in normal alkane-grown cells of hydrocarbon-utilizing Candida yeasts, and that the microbodies multiplied by division in these cells. These features of Candida yeasts seem to provide a useful model system for studies on the biogenesis of the microbody. Subsequently, we have succeeded in isolation of Candida microbodies in an apparently native state, as judged biochemically and morphologically. The presence of DNA in the purified microbody fraction thus obtained was proved by the diphenylamine method. DNA molecule of about 15 urn in contour length was released from an isolated microbody. The physicochemical analyses of the microbody DNA revealed that its buoyant density differed from nuclear and mitochondrial DNAs. All these results lead us to the possibility that there is a novel type of DNA in microbodies.

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Azocoll protease activity assay

Protocol Exchange ◽

10.1038/nprot.2007.484 ◽

2007 ◽

Cited By ~ 4

Author(s):

Naxin Jiang ◽

Nguan Soon Tan ◽

Bow Ho ◽

Jeak Ling Ding

Keyword(s):

Protease Activity ◽

Activity Assay

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Catalase Activity in Bottom Sediments of the Water Bodies of Different Types in the Urbanized Territory

Hydrobiological Journal ◽

10.1615/hydrobj.v56.i1.60 ◽

2020 ◽

Vol 56 (1) ◽

pp. 70-80

Author(s):

Ye. V. Starosyla

Keyword(s):

Bottom Sediments ◽

Catalase Activity ◽

Water Bodies ◽

Urbanized Territory ◽

Different Types

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CHARACTERISTICS OF FREE-RADICAL PROCESSES IN THE ORGANISMS OF SOME PREDATORY FISH SPECIES IN DIFFERENT SEASONS OF THE YEAR

VESTNIK OF ASTRAKHAN STATE TECHNICAL UNIVERSITY SERIES FISHING INDUSTRY ◽

10.24143/2073-5529-2017-1-117-123 ◽

2017 ◽

pp. 117-123

Author(s):

Nikolay Aleksandrovich Pudovkin ◽

Peter Vladimirovich Smutnev

Keyword(s):

Fish Species ◽

Catalase Activity ◽

Perca Fluviatilis ◽

Selenium Concentration ◽

The Body ◽

Predatory Fish ◽

Volga River ◽

Silurus Glanis ◽

Average Value ◽

Pike Perch

The authors of the article have studied the content of the elements of anti-oxidant system (malondialdehyde, catalase, selenium) in tissues of the internal organs (gills, intestine, muscles, liver, swimbladder, scales) in some species of predatory fish (pike Esox lucius (L., 1758), perch Perca fluviatilis (L., 1758), pike-perch Sander lucioperca (L., 1758), catfish Silurus glanis ) widespread in the basin of the Volga river in the Saratov region. The lowest concentration of malondialdehyde in organisms of the studied fish species is observed in fall and winter; the highest - in spring and summer. Catalase activity in gills tissue of a pike raised in 11.8%, cat-fish - 9.1%, pike-perch - 7.5%, perch - 7.8%. In fall (compared to winter) enzyme activity lowering in gonads of pike-perch makes 16.3%, in gonads of perch - 14.4%. In other tissues there were not observed any evident changes of catalase activity. Fish species under consideration are listed according to the average value of selenium concentration in organisms, µg/g: pike (0.208) > catfish (0.207) > pike-perch (0.196) > perch (0.178). According to the average value of the selenium accumulation in the body in different season all the studied species can be placed in the following order, µg/g: winter-pike (0.132) > pike-perch (0.136) > perch and catfish (0.142); spring - pike-perch (0.190) > perch (0.191) > pike (0.208) > catfish (0.209); summer - perch (0.186) > pike-perch (0.190) > catfish and pike (0.203); autumn - perch (0.193) > pike-perch (0.268) > > catfish (0.274) > pike (0.289).

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Drought stress and osmo-priming effects on physiological and biochemical characteristics of Ocimum basilicum

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v6i1.5397 ◽

2016 ◽

Vol 6 (1) ◽

pp. 905-913

Author(s):

Bahram Majd Nassiry ◽

Neda Mohammadi

Keyword(s):

Drought Stress ◽

Total Protein ◽

Catalase Activity ◽

Seedling Emergence ◽

Seed Priming ◽

Ocimum Basilicum ◽

Field Development ◽

Physiological And Biochemical Characteristics ◽

Germination Characteristics ◽

And Control

Â Â Â One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development. Seed priming technique has been known as a challenge to improve germination and seedling emergence under different environmental stresses. The objectives of this research were to evaluate the effects of osmo-priming on germination characteristics and changes of proline, protein and catalase activity of Ocimum basilicum seeds. Results showed that drought stress reduced the germination characteristics and drought stress in -8 bar was the critical stress.Â Priming treatments were include KNO3, PEG and NaCl by 0, -4 and -8 bar concentrations. The seeds were primed with those materials for 8 and 16 hours. The highest germination characteristics were obtained from nitrate potassium in -8 bar for 16 hours priming. Therefore the best seed treatment under drought stress during germination was obtained from the osmo-primed with -8 bar nitrate potassium for 16 hours. The drought stress increased proline and catalase activity but reduced total protein. Priming treatment increases proline, total protein and catalase activity under drought and control conditions. It is concluded that priming results in improvement in germination components of Ocimum basilicum in drought stress conditions and increases the resistance to drought stress with improvement of proline, protein and catalase activity in germination phase.

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