Detection of Citrus tristeza virus and Coinfecting Viroids

2019 ◽  
pp. 67-78
Author(s):  
Maria Saponari ◽  
Stefania Zicca ◽  
Giuliana Loconsole ◽  
Beatriz Navarro ◽  
Francesco Di Serio
Keyword(s):  
Citrus Tristeza Virus ◽  
Tristeza Virus ◽  
Citrus Tristeza

First report of citrus tristeza virus in commercial citrus orchards in Tunisia

2021 ◽  
Author(s):  
Asma Najar ◽  
Imen Hamdi ◽  
Souad Mahmoud ◽  
Lassaad Medhioub ◽  
Imed Jaouadi ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
First Report ◽  
Citrus Orchards ◽  
Tristeza Virus ◽  
Citrus Tristeza

A model for the spread and control of citrus tristeza virus disease

1989 ◽  
Vol 16 (3) ◽  
pp. 315-320
Author(s):  
Ruth Marcus ◽  
Hovav Talpaz ◽  
Moshe Bar-Joseph
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
And Control ◽  
Tristeza Virus ◽  
Citrus Tristeza

Sscp analysis of variations in haplotypes of citrus tristeza virus isolated from yuzu (Citrus junos) in geographically separate regions of Korea

2006 ◽  
Vol 49 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Dae Hyun Kim ◽  
Hye Kyung Shim ◽  
Jae Wook Hyeon ◽  
Hyeog Mo Kwon ◽  
Kwang Sik Kim ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Sscp Analysis ◽  
Citrus Junos ◽  
Tristeza Virus ◽  
Citrus Tristeza

Production of Antibodies to Citrus Tristeza Virus in Transgenic Citrus

1995 ◽  
Author(s):  
Richard Lee ◽  
Moshe Bar-Joseph ◽  
K.S. Derrick ◽  
Aliza Vardi ◽  
Roland Brlansky ◽  
...  
Keyword(s):  
Citrus Tristeza Virus ◽  
Virus Disease ◽  
Biological Activities ◽  
Cp Gene ◽  
Single Domain Antibodies ◽  
Tristeza Virus ◽  
Stem Pitting ◽  
Citrus Tristeza

Citrus tristeza virus (CTV) is the most important virus disease of citrus in the world. CTV causes death of trees on sour orange rootstock and/or stem pitting of scions regardless of rootstock which results in trees of low vigor, reduced yield with reduction in size and quality of fruit. The purpose of this project was to produce monoclonal antibodies (MABs) to CTV coat protein (CP), develop single domain antibodies (dAbs) or Fab fragments which neutralize the infection by binding to the virus, and to produce transformed plants which express the dAbs. The objectives of this research have been met and putative transgenic tobacco and citrus plants have been developed. These putative transgenic plants are presently undergoing evaluation to determine the level of dAbs expression and to determine their resistance to CTV. Additionally, the CTV genome has been sequenced and the CP gene of several biologically characterized CTV strains molecular characterized. This has indicated a correlation between CP sequence homology and biological activity, and the finding of DI RNAs associated with some CTV strains. Several MABs have been produced which enable broad spectrum identification of CTV strains while other MABs enable differentiation between mild and severe strains. The use of selected MAbs and determination of the CP gene sequence has enabled predictions of biological activities of unknown CTV isolates. The epitopes of two MABs, one reacting selectively with severe CTV strains and the other reacting with all strains, have been characterized at the molecular level.

scholarly journals Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program

2013 ◽  
Vol 70 (2) ◽  
pp. 116-124 ◽  
Author(s):  
Carlos Alexandre Zanutto ◽  
Maria Júlia Corazza ◽  
William Mário de Carvalho Nunes ◽  
Gerd Walter Müller
Keyword(s):  
Citrus Tristeza Virus ◽  
Protective Capacity ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals Eliminação de vírus do complexo sorose dos citros por microenxertia associada a termoterapia

2002 ◽  
Vol 27 (3) ◽  
pp. 306-308 ◽  
Author(s):  
SÉRGIO A. CARVALHO ◽  
FRANCISCA A. SANTOS ◽  
MARCOS A. MACHADO
Keyword(s):  
Citrus Tristeza Virus ◽  
Citrus Exocortis Viroid ◽  
Tristeza Virus ◽  
Citrus Tristeza

A microenxertia de ápices caulinares tem sido utilizada com 100% de sucesso na eliminação do vírus da tristeza (Citrus tristeza virus) e dos viróides da exocorte (Citrus exocortis viroid - CEVd) e cachexia-xiloporose de materiais do Banco Ativo de Germoplasma de Citros do Centro de Citricultura Sylvio Moreira CCSM-IAC. Para o complexo da sorose, entretanto, esta técnica tem apresentado somente 60% de eficiência, indicando a necessidade de sua associação com termoterapia para garantir a eliminação viral. Para tanto, mudas originadas de borbulhas infetadas com sorose foram mantidas em câmara climática com 16 h de luz a 38 ºC e 8 h no escuro a 32 ºC e utilizadas para a obtenção dos ápices caulinares empregados na microenxertia. Após o pegamento, o conjunto micro porta-enxerto e brotação foi sobre-enxertado em limoeiro (Citrus limonia) 'Cravo' com sete meses de idade e mantido em condições de casa de vegetação. Clones de laranjeiras doces (Citrus sinsensis) 'Lima', 'Rubi', 'Piralima', 'Salustiana', 'João Nunes', 'Rosa' e 'Pêra Caire' tratados desta maneira, comprovaram eficiência de 100% de eliminação do complexo sorose, conforme indexação realizada empregando-se laranjeira 'Do Céu' como planta indicadora.

scholarly journals Genetic Diversity and Phylogenetic Analysis of Citrus tristeza virus Isolates from Turkey

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Gözde Erkiş-Güngör ◽  
Bayram Çevik
Keyword(s):  
Phylogenetic Analysis ◽  
Citrus Tristeza Virus ◽  
Molecular Characteristics ◽  
Cloning And Sequencing ◽  
Phylogenetic Groups ◽  
The 1960S ◽  
Tristeza Virus ◽  
Virus Isolates ◽  
Stem Pitting ◽  
Citrus Tristeza

The presence of Citrus tristeza virus (CTV) in Turkey has been known since the 1960s and the virus was detected in all citrus growing regions of the country. Even though serological and biological characteristics of CTV have been studied since the 1980s, molecular characteristics of CTV isolates have not been studied to date in Turkey. In this study, molecular characteristics of 15 CTV isolates collected from different citrus growing regions of Turkey were determined by amplification, cloning, and sequencing of their major coat protein (CP) genes. The sequence analysis showed that the CP genes were highly conserved among Turkish isolates. However, isolates from different regions showed more genetic variation than isolates from the same region. Turkish isolates were clustered into three phylogenetic groups showing no association with geographical origins, host, or symptoms induced in indicator plants. Phylogenetic analysis of Turkish isolates with isolates from different citrus growing regions of the world including well-characterized type isolates of previously established strain specific groups revealed that some Turkish isolates were closely related to severe quick decline or stem pitting isolates. The results demonstrated that although CTV isolates from Turkey are considered biologically mild, majority of them contain severe components potentially causing quick decline or stem pitting.

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