Immunogenicity and Protective Capacity of a Virus-like Particle Vaccine against Chlamydia trachomatis Type 3 Secretion System Tip Protein, CT584

An effective vaccine against Chlamydia trachomatis is urgently needed as infection rates continue to rise and C. trachomatis causes reproductive morbidity. An obligate intracellular pathogen, C. trachomatis employs a type 3 secretion system (T3SS) for host cell entry. The tip of the injectosome is composed of the protein CT584, which represents a potential target for neutralization with vaccine-induced antibody. Here, we investigate the immunogenicity and efficacy of a vaccine made of CT584 epitopes coupled to a bacteriophage virus-like particle (VLP), a novel platform for Chlamydia vaccines modeled on the success of HPV vaccines. Female mice were immunized intramuscularly, challenged transcervically with C. trachomatis, and assessed for systemic and local antibody responses and bacterial burden in the upper genital tract. Immunization resulted in a 3-log increase in epitope-specific IgG in serum and uterine homogenates and in the detection of epitope-specific IgG in uterine lavage at low levels. By contrast, sera from women infected with C. trachomatis and virgin controls had similarly low titers to CT584 epitopes, suggesting these epitopes are not systemically immunogenic during natural infection but can be rendered immunogenic by the VLP platform. C. trachomatis burden in the upper genital tract of mice varied after active immunization, yet passive protection was achieved when immune sera were pre-incubated with C. trachomatis prior to inoculation into the genital tract. These data demonstrate the potential for antibody against the T3SS to contribute to protection against C. trachomatis and the value of VLPs as a novel platform for C. trachomatis vaccines.

Scour Protection of Submarine Pipelines Using Ionic Soil Stabilizer Solidified Soil

A novel scour protection approach for pipeline using the Ionic Soil Stabilizer (ISS) solidified soil was proposed in this study. The ISS-solidified slurry can be poured adjacent to the pipeline immediately after it was placed, or in the growing scour holes. In the present study, the first type was utilized as the scour protection layer around the pipeline. A series of laboratory flume tests were conducted to validate the protective capacity of ISS-solidified slurry for the pipeline in waves and combined waves and current. Then, the scanning electron microscope (SEM) tests and pore size tests were carried out, respectively, to investigate the mechanism of ISS-solidified slurry for scour protection around the pipeline. Finally, the effects of the ISS-solidified layer for liquefaction stability of non-cohesive subsoil were evaluated. The results indicated that the ISS-solidified slurry is a reliable, economic approach for scour protection around pipelines in the ocean environment. It is noteworthy that if a non-cohesive soil layer underlies the ISS-solidified slurry, it is vulnerable to suffer accumulated liquefaction due to the dense crust structure of the ISS-solidified layer, so the adverse effects for accumulated liquefaction should be considered carefully due to the set of the ISS-solidified layer.

Polylactic acid based Janus membranes with asymmetric wettability for directional moisture transport with enhanced UV protective capabilities

The PLA-E/TiO2@PDA–PLA Janus membrane with asymmetric wettability exhibits efficient directional moisture transport and excellent UV protective capacity.

Geohydraulic and vulnerability assessment of tropically weathered and fractured gneissic aquifers using combined electrical resistivity and geostatistical methods

Sustainable potable groundwater supplied by aquifers depends on the protective capacity of the strata overlying the aquifer zones and their thicknesses, as well as the nature of the aquifers and the conduit systems. The poor overburden development of the Araromi area of Akungba-Akoko, in the crystalline basement of southwestern Nigeria, restricts most aquifers to shallow depths. Hence, there is a need to investigate the groundwater quality of the tropically weathered and fractured gneissic aquifers in the area. A combined electrical resistivity tomography (ERT) and Schlumberger vertical electrical sounding (VES) technique were employed to assess the groundwater-yielding potential and vulnerability of the aquifer units. The measured geoelectric parameters (i.e., resistivity and thickness values) at the respective VES surveyed stations were used to compute the geohydraulic parameters, such as aquifer resistivity (\({\rho }_{o}\)), hydraulic conductivity (K), transmissivity (T), porosity (\(\phi\)), permeability (\({\Psi }\)), hydraulic resistance (\({\text{K}}_{R}\)), and longitudinal conductance (S). In addition, regression analysis was employed to establish the correlations between the K and other geohydraulic parameters to achieve the objectives of this study. The subsurface lithostratigraphic units of the studied site were delineated as the motley topsoil, weathered layers, partially weathered/fractured bedrock units, and the fresh bedrock, based on the ERT and the A, H, AK, HA, and KQ curve models. The K model regression-assisted analysis showed that the \({\rho }_{o}\), T, \(\phi\), \({\Psi }\), and S contributed about 81.7%, 3.31%. 96.6%, 100%, and 11.63%, respectively, of the determined K values for the study area. The results, except T and S, have strong high positive correlations with the K of the aquifer units; hence, accounted for the recorded high percentages. The aquifer units in the area were classified as low to moderate groundwater-yielding potential due to the thin overburden, with an average depth of <4 m. However, the deep-weathered and fractured aquifer zones with depths ranging from about 39–55 m could supply high groundwater yield for sustainable exploitation. The estimated S values, i.e., 0.0226–0.1926 mho, for aquifer protective capacity ratings rated the aquifer units in the area as poor/weak to moderately high with extremely high to high aquifer vulnerability index, based on the estimated low Log \({\text{K}}_{R}\) of about 0.01–1.77 years. Hence, intended wells/boreholes in the study area and its environs, as well as any environments with similar geohydraulic and vulnerability characteristics, should be properly constructed to adequately prevent surface and subsurface infiltrating contaminants.

Geohydraulic and vulnerability assessment of tropically weathered and fractured gneissic aquifers using combined electrical resistivity and geostatistical methods

Sustainable potable groundwater supplied by aquifers depends on the protective capacity of the strata overlying the aquifer zones and their thicknesses, as well as the nature of the aquifers and the conduit systems. The poor overburden development of the Araromi area of Akungba-Akoko, in the crystalline basement of southwestern Nigeria, restricts most aquifers to shallow depths. Hence, there is a need to investigate the groundwater quality of the tropically weathered and fractured gneissic aquifers in the area. A combined electrical resistivity tomography (ERT) and Schlumberger vertical electrical sounding (VES) technique were employed to assess the groundwater-yielding potential and vulnerability of the aquifer units. The measured geoelectric parameters (i.e., resistivity and thickness values) at the respective VES surveyed stations were used to compute the geohydraulic parameters, such as aquifer resistivity (\({\rho }_{o}\)), hydraulic conductivity (K), transmissivity (T), porosity (\(\phi\)), permeability (\({\Psi }\)), hydraulic resistance (\({\text{K}}_{R}\)), and longitudinal conductance (S). In addition, regression analysis was employed to establish the correlations between the K and other geohydraulic parameters to achieve the objectives of this study. The subsurface lithostratigraphic units of the studied site were delineated as the motley topsoil, weathered layers, partially weathered/fractured bedrock units, and the fresh bedrock, based on the ERT and the A, H, AK, HA, and KQ curve models. The K model regression-assisted analysis showed that the \({\rho }_{o}\), T, \(\phi\), \({\Psi }\), and S contributed about 81.7%, 3.31%. 96.6%, 100%, and 11.63%, respectively, of the determined K values for the study area. The results, except T and S, have strong high positive correlations with the K of the aquifer units; hence, accounted for the recorded high percentages. The aquifer units in the area were classified as low to moderate groundwater-yielding potential due to the thin overburden, with an average depth of <4 m. However, the deep-weathered and fractured aquifer zones with depths ranging from about 39–55 m could supply high groundwater yield for sustainable exploitation. The estimated S values, i.e., 0.0226–0.1926 mho, for aquifer protective capacity ratings rated the aquifer units in the area as poor/weak to moderately high with extremely high to high aquifer vulnerability index, based on the estimated low Log \({\text{K}}_{R}\) of about 0.01–1.77 years. Hence, intended wells/boreholes in the study area and its environs, as well as any environments with similar geohydraulic and vulnerability characteristics, should be properly constructed to adequately prevent surface and subsurface infiltrating contaminants.

On increase in stability of superhydrophobic oleic acid layers on surface of D16 aluminum alloy

The possibility of increasing the stability of SHP layers formed from ethanol solutions of oleic acid (OlA) by layer-by-layer modification of the laser-textured surface of the alloy D16 by ethanol solutions of trialkoxysilanes (vinyltrimethoxysilane (VS), octyltriethoxysilane (OTES)) is discussed. It is shown that OTES is more effective than VS in achieving the SHF state of the alloy surface. The protective ability of the coatings was evaluated by polarization measurements in 0,05 M NaCl water solution and corrosion tests in a salt spray chamber. It is shown that the two-stage modification of the alloy surface in solutions of OTES and OlA at Cin = 10 mmol / l, allows one to surpass the protective capacity of individual components, and τcor = 498 h. It is important that the SHP properties of the coating are preserved for 240 hours of testing under salt spray chamber conditions.

A booster dose of an inactivated vaccine increases neutralizing antibodies and T cell responses against SARS-CoV-2

Numerous vaccines have been generated to decrease the morbidity and mortality of COVID-19. CoronaVac® is an inactivated SARS-CoV-2 vaccine approved by the World Health Organization (WHO) to prevent COVID-19 that has safety and immunogenicity profiles described in different clinical trials. We previously reported an increase in levels of neutralizing antibodies two- and four-weeks after administering two doses of CoronaVac® in a two-week interval (0-14 day) vaccination schedule, as compared to pre-immune sera in adults in the Chilean population that are participating in phase 3 clinical trial. Here we report the levels of antibodies directed against the Receptor Binding Domain of the SARS-CoV-2 spike protein comparing their neutralizing capacities and the cellular response at five months after the second dose and four weeks after a booster (third) dose in volunteers immunized with two doses of CoronaVac®in a four-week interval (0-28 day) vaccination schedule. We observed a decrease in the levels of anti-SARS-CoV-2 antibodies with neutralizing capacities five months after the second dose (GMU 39.0 95% confidence interval (CI)(32.4-47.0), which increased up to 12 times at four weeks after the booster dose (GMU 499.4, 95% CI=370.6-673.0). Equivalent results were observed in adults aged 18-59 years old and individuals ≥60 years old. In the case of cellular response, we observed that activation of specific CD4+ T cells increases in time and reaches its maximum at four weeks after the booster dose in both groups. Our results support the notion that a booster dose of the SARS-CoV-2 inactivated vaccine increases the levels of neutralizing antibodies and the specific cellular response in adults of both groups, which is likely to boost the protective capacity of these vaccines against COVID-19.

A stem-loop RNA RIG-I agonist protects against acute and chronic SARS-CoV-2 infection in mice

As SARS-CoV-2 continues to cause morbidity and mortality around the world, there is an urgent need for the development of effective medical countermeasures. Here, we assessed the antiviral capacity of a minimal RIG-I agonist, stem-loop RNA 14 (SLR14), in viral control, disease prevention, post-infection therapy, and cross-variant protection in mouse models of SARS-CoV-2 infection. A single dose of SLR14 prevented viral infection in the lower respiratory tract and development of severe disease in a type I interferon (IFN-I)–dependent manner. SLR14 demonstrated remarkable prophylactic protective capacity against lethal SARS-CoV-2 infection and retained considerable efficacy as a therapeutic agent. In immunodeficient mice carrying chronic SARS-CoV-2 infection, SLR14 elicited near-sterilizing innate immunity in the absence of the adaptive immune system. In the context of infection with variants of concern (VOCs), SLR14 conferred broad protection against emerging VOCs. These findings demonstrate the therapeutic potential of SLR14 as a host-directed, broad-spectrum antiviral for early post-exposure treatment and treatment of chronically infected immunosuppressed patients.

Tomato Yellow Leaf Curl Virus (TYLCV) Promotes Plant Tolerance to Drought

A growing body of research points to a positive interplay between viruses and plants. Tomato yellow curl virus (TYLCV) is able to protect tomato host plants against extreme drought. To envisage the use of virus protective capacity in agriculture, TYLCV-resistant tomato lines have to be infected first with the virus before planting. Such virus-resistant tomato plants contain virus amounts that do not cause disease symptoms, growth inhibition, or yield loss, but are sufficient to modify the metabolism of the plant, resulting in improved tolerance to drought. This phenomenon is based on the TYLCV-dependent stabilization of amounts of key osmoprotectants induced by drought (soluble sugars, amino acids, and proteins). Although in infected TYLCV-susceptible tomatoes, stress markers also show an enhanced stability, in infected TYLCV-resistant plants, water balance and osmolyte homeostasis reach particularly high levels. These tomato plants survive long periods of time during water withholding. However, after recovery to normal irrigation, they produce fruits which are not exposed to drought, similarly to the control plants. Using these features, it might be possible to cultivate TYLCV-resistant plants during seasons characterized by water scarcity.