Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR

2019 ◽  
pp. 139-151 ◽  
Author(s):  
Francesca Salvianti ◽  
Filomena Costanza ◽  
Gemma Sonnati ◽  
Pamela Pinzani
Keyword(s):  
Real Time ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr

scholarly journals Molecular characterization of circulating tumor cells in large quantities of contaminating leukocytes by a multiplex real-time PCR

2008 ◽  
Vol 118 (3) ◽  
pp. 455-468 ◽  
Author(s):  
Anieta M. Sieuwerts ◽  
Jaco Kraan ◽  
Joan Bolt-de Vries ◽  
Petra van der Spoel ◽  
Bianca Mostert ◽  
...  
Keyword(s):  
Real Time ◽  
Tumor Cells ◽  
Molecular Characterization ◽  
Circulating Tumor Cells ◽  
Real Time Pcr

scholarly journals Improved HF183 Quantitative Real-Time PCR Assay for Characterization of Human Fecal Pollution in Ambient Surface Water Samples

2014 ◽  
Vol 80 (10) ◽  
pp. 3086-3094 ◽  
Author(s):  
Hyatt C. Green ◽  
Richard A. Haugland ◽  
Manju Varma ◽  
Hana T. Millen ◽  
Mark A. Borchardt ◽  
...  
Keyword(s):  
Real Time ◽  
Surface Waters ◽  
Real Time Pcr ◽  
Fecal Pollution ◽  
Qpcr Assay ◽  
Taqman Assay ◽  
Reference Database ◽  
Quantitative Real Time Pcr ◽  
Ambient Surface

ABSTRACTQuantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genusBacteroidesare among the most widely used methods for the characterization of human fecal pollution in ambient surface waters. In this study, we show that a current TaqMan HF183 qPCR assay (HF183/BFDrev) routinely forms nonspecific amplification products and introduce a modified TaqMan assay (HF183/BacR287) that alleviates this problem. The performance of each qPCR assay was compared in head-to-head experiments investigating limits of detection, analytical precision, predicted hybridization to 16S rRNA gene sequences from a reference database, and relative marker concentrations in fecal and sewage samples. The performance of the modified HF183/BacR287 assay is equal to or improves upon that of the original HF183/BFDrev assay. In addition, a qPCR chemistry designed to combat amplification inhibition and a multiplexed internal amplification control are included. In light of the expanding use of PCR-based methods that rely on the detection of extremely low concentrations of DNA template, such as qPCR and digital PCR, the new TaqMan HF183/BacR287 assay should provide more accurate estimations of human-derived fecal contaminants in ambient surface waters.

Characterization of fecal concentrations in human and other animal sources by physical, culture-based, and quantitative real-time PCR methods

2013 ◽  
Vol 47 (18) ◽  
pp. 6873-6882 ◽  
Author(s):  
Jared S. Ervin ◽  
Todd L. Russell ◽  
Blythe A. Layton ◽  
Kevan M. Yamahara ◽  
Dan Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Physical Culture ◽  
Quantitative Real Time Pcr
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