Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult-to-Drug Targets

Methods in Molecular Biology - Genotype Phenotype Coupling ◽

10.1007/978-1-4939-9853-1_21 ◽

2019 ◽

pp. 363-396 ◽

Cited By ~ 1

Author(s):

Zachary R. Crook ◽

Gregory P. Sevilla ◽

Andrew J. Mhyre ◽

James M. Olson

Keyword(s):

Drug Targets ◽

Surface Display ◽

Peptide Libraries

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Epitope mapping and affinity purification of monospecific antibodies by Escherichia coli cell surface display of gene-derived random peptide libraries

Journal of Immunological Methods ◽

10.1016/s0022-1759(01)00461-6 ◽

2001 ◽

Vol 257 (1-2) ◽

pp. 163-173 ◽

Cited By ~ 43

Author(s):

Andreas Christmann ◽

Alexander Wentzel ◽

Christiane Meyer ◽

Gregor Meyers ◽

Harald Kolmar

Keyword(s):

Escherichia Coli ◽

Cell Surface ◽

Epitope Mapping ◽

Affinity Purification ◽

Surface Display ◽

Cell Surface Display ◽

Peptide Libraries ◽

Random Peptide ◽

Random Peptide Libraries ◽

Monospecific Antibodies

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SAROTUP: Scanner and Reporter of Target-Unrelated Peptides

Journal of Biomedicine and Biotechnology ◽

10.1155/2010/101932 ◽

2010 ◽

Vol 2010 ◽

pp. 1-7 ◽

Cited By ~ 43

Author(s):

Jian Huang ◽

Beibei Ru ◽

Shiyong Li ◽

Hao Lin ◽

Feng-Biao Guo

Keyword(s):

Phage Display ◽

Epitope Mapping ◽

Surface Display ◽

Epitope Prediction ◽

Peptide Libraries ◽

Screening System ◽

Web Tool ◽

Random Peptide ◽

Random Peptide Libraries ◽

Convenient Approach

As epitope mimics, mimotopes have been widely utilized in the study of epitope prediction and the development of new diagnostics, therapeutics, and vaccines. Screening the random peptide libraries constructed with phage display or any other surface display technologies provides an efficient and convenient approach to acquire mimotopes. However, target-unrelated peptides creep into mimotopes from time to time through binding to contaminants or other components of the screening system. In this study, we present SAROTUP, a free web tool for scanning, reporting and excluding possible target-unrelated peptides from real mimotopes. Preliminary tests show that SAROTUP is efficient and capable of improving the accuracy of mimotope-based epitope mapping. It is also helpful for the development of mimotope-based diagnostics, therapeutics, and vaccines.

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