Development of Human Keratinocyte Colonies for Confocal Microscopy and for Study of Calcium Effects on Growth Differentiation and Sulfur Mustard Lesions

1999 ◽  
pp. 165-174 ◽  
Author(s):  
Robert J. Werrlein ◽  
Tracey A. Hamilton ◽  
Janna S. Madren-Whalley
Keyword(s):  
Confocal Microscopy ◽  
Sulfur Mustard ◽  
Human Keratinocyte ◽  
Calcium Effects

Sulfur mustard downregulates iNOS expression to inhibit wound healing in a human keratinocyte model

2008 ◽  
Vol 49 (3) ◽  
pp. 207-216 ◽  
Author(s):  
Hiroshi Ishida ◽  
Radharaman Ray ◽  
Prabhati Ray
Keyword(s):  
Wound Healing ◽  
Sulfur Mustard ◽  
Inos Expression ◽  
Human Keratinocyte

Protective effects of the thiol compounds GSH and NAC against sulfur mustard toxicity in a human keratinocyte cell line

2016 ◽  
Vol 244 ◽  
pp. 35-43 ◽  
Author(s):  
Frank Balszuweit ◽  
Georg Menacher ◽  
Annette Schmidt ◽  
Kai Kehe ◽  
Tanja Popp ◽  
...  
Keyword(s):  
Cell Line ◽  
Sulfur Mustard ◽  
Protective Effects ◽  
Thiol Compounds ◽  
Human Keratinocyte ◽  
Human Keratinocyte Cell Line ◽  
Keratinocyte Cell

Human Keratinocyte Inflammatory Transcript Gene Activity Following Sulfur Mustard

2003 ◽  
Author(s):  
James Clark ◽  
Douglas Avery ◽  
Kashif Ali ◽  
Hilma Benjamin ◽  
Claire Levine ◽  
...  
Keyword(s):  
Sulfur Mustard ◽  
Gene Activity ◽  
Human Keratinocyte ◽  
Transcript Gene

scholarly journals Expression of Dominant-negative Fas-associated Death Domain Blocks Human Keratinocyte Apoptosis and Vesication Induced by Sulfur Mustard

2002 ◽  
Vol 278 (10) ◽  
pp. 8531-8540 ◽  
Author(s):  
Dean S. Rosenthal ◽  
Alfredo Velena ◽  
Feng-Pai Chou ◽  
Richard Schlegel ◽  
Radharaman Ray ◽  
...  
Keyword(s):  
Sulfur Mustard ◽  
Dominant Negative ◽  
Death Domain ◽  
Human Keratinocyte

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

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