Computational Analysis of Quantitative Proteomics Data Using Stable Isotope Labeling

2007 ◽  
pp. 177-189 ◽  
Author(s):  
Michael J. MacCoss ◽  
Christine C. Wu
Keyword(s):  
Stable Isotope ◽  
Quantitative Proteomics ◽  
Computational Analysis ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Proteomics Data

scholarly journals Stable Isotope Labeling ofArabidopsis thalianaCells and Quantitative Proteomics by Mass Spectrometry

2005 ◽  
Vol 4 (11) ◽  
pp. 1697-1709 ◽  
Author(s):  
Albrecht Gruhler ◽  
Waltraud X. Schulze ◽  
Rune Matthiesen ◽  
Matthias Mann ◽  
Ole N. Jensen
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Quantitative Proteomics ◽  
Isotope Labeling ◽  
Stable Isotope Labeling

Toward improved peptide feature detection in quantitative proteomics using stable isotope labeling

2015 ◽  
Vol 9 (7-8) ◽  
pp. 706-714 ◽  
Author(s):  
Lars Nilse ◽  
Florian Christoph Sigloch ◽  
Martin L. Biniossek ◽  
Oliver Schilling
Keyword(s):  
Stable Isotope ◽  
Quantitative Proteomics ◽  
Feature Detection ◽  
Isotope Labeling ◽  
Stable Isotope Labeling

scholarly journals Quantitative Proteomics in Drosophila with Holidic Stable-Isotope Labeling of Amino Acids in Fruit Flies (SILAF)

2020 ◽  
pp. 75-87
Author(s):  
Florian A. Schober ◽  
Ilian Atanassov ◽  
Christoph Freyer ◽  
Anna Wredenberg
Keyword(s):  
Amino Acids ◽  
Stable Isotope ◽  
Posttranslational Modifications ◽  
Quantitative Proteomics ◽  
Isotope Labeling ◽  
Cost Effective ◽  
Fruit Flies ◽  
Stable Isotope Labeling ◽  
Functional Studies ◽  
Wet Lab

AbstractProtein-focused research has been challenging in Drosophila melanogaster due to few specific antibodies for Western blotting and the lack of effective labeling methods for quantitative proteomics. Herein, we describe the preparation of a holidic medium that allows stable-isotope labeling of amino acids in fruit flies (SILAF). Furthermore, in this chapter, we provide a protocol for mitochondrial enrichments from Drosophila larvae and flies together with a procedure to generate high-quality peptides for further analysis by mass spectrometry. Samples obtained following this protocol can be used for various functional studies such as comprehensive proteome profiling or quantitative analysis of posttranslational modifications upon enrichment. SILAF is based on standard fly routines in a basic wet lab environment and provides a flexible and cost-effective tool for quantitative protein expression analysis.

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