Natural Compounds Obtained Through Centrifugal Molecular Distillation

Author(s):  
Vanessa Mayumi Ito ◽  
Patricia Fazzio Martins ◽  
César Benedito Batistella ◽  
Rubens Maciel Filho ◽  
Maria Regina Wolf Maciel
1996 ◽  
Vol 131 (1-3) ◽  
pp. 716-726
Author(s):  
Vanessa Mayumi Ito ◽  
Patricia Fazzio Martins ◽  
César Benedito Batistella ◽  
Rubens Maciel Filho ◽  
Maria Regina Wolf Maciel

2006 ◽  
Vol 131 (1-3) ◽  
pp. 716-726 ◽  
Author(s):  
Vanessa Mayumi Ito ◽  
Patricia Fazzio Martins ◽  
César Benedito Batistella ◽  
Rubens Maciel Filho ◽  
Maria Regina Wolf Maciel

Author(s):  
David L. Spector ◽  
Robert J. Derby

Studies in our laboratory are involved in evaluating the structural and functional organization of the mammalian cell nucleus. Since several major classes (U1, U2, U4/U6, U5) of small nuclear ribonucleoprotein particles (snRNPs) play a crucial role in the processing of pre-mRNA molecules, we have been interested in the localization of these particles within the cell nucleus. Using pre-embedding immunoperoxidase labeling combined with 3-dimensional reconstruction, we have recently shown that nuclear regions enriched in snRNPs form a reticular network within the nucleoplasm which extends between the nucleolar surface and the nuclear envelope. In the present study we were inte rested in extending these nuclear localizations using cell preparation techniques which avoid slow penetration of fixatives, chemical crosslinking of potential antigens and solvent extraction. CHOC 400 cells were cryofixed using a CF 100 ultra rapid cooling device (LifeCell Corp.). After cryofixation cells were molecular distillation dried, vapor osmicated, in filtra ted in 100% Spurr resin in vacuo and polymerized in molds a t 60°C. Using this procedure we were able to evaluate the distribution of snRNPs in resin embedded cells which had not been chemically fixed, incubated in cryoprotectants or extracted with solvents.


2021 ◽  
Author(s):  
Manuela Oliverio ◽  
Monica Nardi ◽  
Maria Luisa Di Gioia ◽  
Paola Costanzo ◽  
Sonia Bonacci ◽  
...  

Semi-synthesis is an effective strategy to obtain both natural and synthetic analogues of the olive secoiridoids, starting from easy accessible natural compounds.


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
C Tamvakopoulos ◽  
ZD Sofianos ◽  
AL Skaltsounis ◽  
M Kritsanida

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
H Derendorf
Keyword(s):  

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
C Si ◽  
L Wu ◽  
S Liu ◽  
G Xu ◽  
X Ren ◽  
...  

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
C Si ◽  
X Ren ◽  
S Liu ◽  
G Xu ◽  
J Jiang

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
HG Anlar ◽  
M Bacanli ◽  
N Basaran ◽  
AA Basaran

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