Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis

2009 ◽  
pp. 51-63 ◽  
Author(s):  
Ian K. Moon ◽  
Michael B. Jarstfer
Keyword(s):  
Gel Electrophoresis ◽  
Native Gel

scholarly journals Subunit composition of CP43-less photosystem II complexes of Synechocystis sp. PCC 6803: implications for the assembly and repair of photosystem II

2012 ◽  
Vol 367 (1608) ◽  
pp. 3444-3454 ◽  
Author(s):  
M. Boehm ◽  
J. Yu ◽  
V. Reisinger ◽  
M. Beckova ◽  
L. A. Eichacker ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Gel Electrophoresis ◽  
Photochemical Activity ◽  
Subunit Composition ◽  
Null Mutant ◽  
Trap Potential ◽  
Native Gel ◽  
Oxygen Evolving ◽  
Pcc 6803

Photosystem II (PSII) mutants are useful experimental tools to trap potential intermediates involved in the assembly of the oxygen-evolving PSII complex. Here, we focus on the subunit composition of the RC47 assembly complex that accumulates in a psbC null mutant of the cyanobacterium Synechocystis sp. PCC 6803 unable to make the CP43 apopolypeptide. By using native gel electrophoresis, we showed that RC47 is heterogeneous and mainly found as a monomer of 220 kDa. RC47 complexes co-purify with small Cab-like proteins (ScpC and/or ScpD) and with Psb28 and its homologue Psb28-2. Analysis of isolated His-tagged RC47 indicated the presence of D1, D2, the CP47 apopolypeptide, plus nine of the 13 low-molecular-mass (LMM) subunits found in the PSII holoenzyme, including PsbL, PsbM and PsbT, which lie at the interface between the two momomers in the dimeric holoenzyme. Not detected were the LMM subunits (PsbK, PsbZ, Psb30 and PsbJ) located in the vicinity of CP43 in the holoenzyme. The photochemical activity of isolated RC47-His complexes, including the rate of reduction of P680 + , was similar to that of PSII complexes lacking the Mn 4 CaO 5 cluster. The implications of our results for the assembly and repair of PSII in vivo are discussed.

scholarly journals Analysis of Polymerase II Elongation Complexes by Native Gel Electrophoresis

2004 ◽  
Vol 279 (22) ◽  
pp. 23223-23228 ◽  
Author(s):  
Zhiqiang Zhang ◽  
Chwen-Huey Wu ◽  
David S. Gilmour
Keyword(s):  
Gel Electrophoresis ◽  
Native Gel

Analysis of Protein-DNA Equilibria by Native Gel Electrophoresis

2007 ◽  
pp. 417-446 ◽  
Author(s):  
Claire A. Adams ◽  
Michael G. Fried
Keyword(s):  
Gel Electrophoresis ◽  
Native Gel

Analysis of proteins by agarose native gel electrophoresis in the presence of solvent additives

2021 ◽  
Author(s):  
Yui Tomioka ◽  
Tsutomu Arakawa ◽  
Teruo Akuta ◽  
Masataka Nakagawa ◽  
Matsujiro Ishibashi
Keyword(s):  
Gel Electrophoresis ◽  
Native Gel

scholarly journals SMA-PAGE: A new method to examine complexes of membrane proteins using SMALP nano-encapsulation and native gel electrophoresis

2019 ◽  
Vol 1861 (8) ◽  
pp. 1437-1445 ◽  
Author(s):  
Naomi L. Pollock ◽  
Megha Rai ◽  
Kailene S. Simon ◽  
Sophie J. Hesketh ◽  
Alvin C.K. Teo ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Gel Electrophoresis ◽  
New Method ◽  
Native Gel
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