Clonal Propagation of Cyclamen persicum Via Somatic Embryogenesis

2009 ◽  
pp. 281-290 ◽  
Author(s):  
Traud Winkelmann
Keyword(s):  
Somatic Embryogenesis ◽  
Clonal Propagation ◽  
Cyclamen Persicum

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum

Commercialization potential of somatic embryogenesis in black spruce tree improvement

1994 ◽  
Vol 70 (5) ◽  
pp. 593-598 ◽  
Author(s):  
G. W. Adams ◽  
M. G. Doiron ◽  
Y. S. Park ◽  
J. M. Bonga ◽  
P. J. Charest
Keyword(s):  
Somatic Embryogenesis ◽  
Clonal Propagation ◽  
Black Spruce ◽  
Tree Improvement ◽  
Embryogenic Tissue ◽  
Improvement Program ◽  
Mature Embryos ◽  
Somatic Plants ◽  
Number Of Individuals ◽  
Potential Tool

The somatic embryogenesis process was evaluated as a potential tool for operational vegetative propagation using individuals from families currently used in the J.D. Irving, Ltd. black spruce tree improvement program. Most families were responsive although the number of individuals within families capable of producing embryogenic tissue (ET) varied greatly (1–70%). Seventy-four percent of the ET clones produced mature embryos and most of these germinated. Greenhouse survival was initially low (11%) but improved in subsequent experiments to 45% as growing regimes were refined. Demonstration plantings of the resulting somatic plants were established at two sites in New Brunswick. A total of 206 clones were cryopreserved. The potential for integrating somatic embryogenesis techniques into tree improvement and stock production programs is discussed. Key words: tree improvement, somatic embryogenesis, clonal propagation, black spruce, biotechnology

scholarly journals Erratum to: Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

2010 ◽  
Vol 28 (2) ◽  
pp. 364-364 ◽  
Author(s):  
Fuhua Bian ◽  
Caixia Zheng ◽  
Funing Qu ◽  
Xueqin Gong ◽  
Cuirong You
Keyword(s):  
Somatic Embryogenesis ◽  
Proteomic Analysis ◽  
Cyclamen Persicum

scholarly journals Genome-wide analysis, transcription factor network approach and gene expression profile of GH3 genes over early somatic embryogenesis in Coffea spp

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Renan Terassi Pinto ◽  
Natália Chagas Freitas ◽  
Wesley Pires Flausino Máximo ◽  
Thiago Bergamo Cardoso ◽  
Débora de Oliveira Prudente ◽  
...  
Keyword(s):  
Amino Acids ◽  
Transcription Factor ◽  
Somatic Embryogenesis ◽  
Amino Acid ◽  
Clonal Propagation ◽  
Family Members ◽  
Network Approach ◽  
Embryogenic Potential ◽  
Auxin Homeostasis ◽  
Early Somatic Embryogenesis

Abstract Background Coffee production relies on plantations with varieties from Coffea arabica and Coffea canephora species. The first, the most representative in terms of coffee consumption, is mostly propagated by seeds, which leads to management problems regarding the plantations maintenance, harvest and processing of grains. Therefore, an efficient clonal propagation process is required for this species cultivation, which is possible by reaching a scalable and cost-effective somatic embryogenesis protocol. A key process on somatic embryogenesis induction is the auxin homeostasis performed by Gretchen Hagen 3 (GH3) proteins through amino acid conjugation. In this study, the GH3 family members were identified on C. canephora genome, and by performing analysis related to gene and protein structure and transcriptomic profile on embryogenic tissues, we point a GH3 gene as a potential regulator of auxin homeostasis during early somatic embryogenesis in C. arabica plants. Results We have searched within the published C. canephora genome and found 17 GH3 family members. We checked the conserved domains for GH3 proteins and clustered the members in three main groups according to phylogenetic relationships. We identified amino acids sets in four GH3 proteins that are related to acidic amino acid conjugation to auxin, and using a transcription factor (TF) network approach followed by RT-qPCR we analyzed their possible transcriptional regulators and expression profiles in cells with contrasting embryogenic potential in C. arabica. The CaGH3.15 expression pattern is the most correlated with embryogenic potential and with CaBBM, a C. arabica ortholog of a major somatic embryogenesis regulator. Conclusion Therefore, one out of the GH3 members may be influencing on coffee somatic embryogenesis by auxin conjugation with acidic amino acids, which leads to the phytohormone degradation. It is an indicative that this gene can serve as a molecular marker for coffee cells with embryogenic potential and needs to be further studied on how much determinant it is for this process. This work, together with future studies, can support the improvement of coffee clonal propagation through in vitro derived somatic embryos.

CYCLAMEN PERSICUM MILL.: SOMATIC EMBRYOGENESIS AND RAPD ANALYSIS OF EMBRYOGENIC CALLUS

2003 ◽  
pp. 137-144 ◽  
Author(s):  
M. Laura ◽  
L. De Benedetti ◽  
.S Bruna ◽  
G. Burchi ◽  
T. Berio ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Embryogenic Callus ◽  
Rapd Analysis ◽  
Cyclamen Persicum

Proteomic Analysis of Somatic Embryogenesis in Cyclamen persicum Mill

2009 ◽  
Vol 28 (1) ◽  
pp. 22-31 ◽  
Author(s):  
Fuhua Bian ◽  
Caixia Zheng ◽  
Funing Qu ◽  
Xueqin Gong ◽  
Cuirong You
Keyword(s):  
Somatic Embryogenesis ◽  
Proteomic Analysis ◽  
Cyclamen Persicum

Proteomic and histological analyses of endosperm development in Cyclamen persicum as a basis for optimization of somatic embryogenesis

Plant Science ◽  
2013 ◽  
Vol 201-202 ◽  
pp. 52-65 ◽  
Author(s):  
Jenniffer Wamaitha Mwangi ◽  
Christina Rode ◽  
Frank Colditz ◽  
Christin Haase ◽  
Hans-Peter Braun ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Endosperm Development ◽  
Cyclamen Persicum

scholarly journals Evaluation of induction of somatic embryogenesis from cotyledonary leaves of Banana Passion fruit (Passiflora mollissima) L.H Bailey

Respuestas ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 31-38
Author(s):  
Oscar José Parra Peñalosa ◽  
Giovanni Orlando Cancino Escalante
Keyword(s):  
Somatic Embryogenesis ◽  
South America ◽  
Genetic Transformation ◽  
High Resistance ◽  
Culture Medium ◽  
Clonal Propagation ◽  
Zygotic Embryogenesis ◽  
Commercial Use ◽  
The Andes ◽  
Embryo Formation

Passiflora mollissima  L.H Bailey is an endemic species from the Andes mountain of Colombia in South America with important edible fruits and medicinal and economical properties. The development of improved micropropagation techniques is necessary to provide rapid and efficient clonal propagation of elite genotypes with high resistance and uniform production, as well as a system that can be used for genetic transformation. For this reason, the investigation focused on the evaluation of induction of somatic embryogenesis in P. mollissima from cotyledonary leaves, the effect of growth regulator concentrations and the orientation of explants on embryo production. Histological analyses of somatic embryogenesis were performed every 10 days after induction over 38 days of exposure to the medium. Results showed somatic embryo formation on Murashige and Skoog, (1962) culture medium supplemented with 4.5 μM 2,4-diclorophenoxyacetic acid plus 4.5 μM 6-benzyladenine. The results obtained are applicable to knowledge of non-zygotic embryogenesis in passionflower of the Andean region, for the purpose of improvement and commercial use. It is noteworthy that this is the first study in the induction and obtaining of embryos in P. mollissima.

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