Periodate Oxidation of Antibodies for Site-Selective Immobilization in Immunoaffinity Chromatography

Author(s):  
David S. Hage
2010 ◽  
Vol 46 (22) ◽  
pp. 3863 ◽  
Author(s):  
Hyun Joo Jung ◽  
Hyegeun Min ◽  
Hyunung Yu ◽  
Tae Geol Lee ◽  
Taek Dong Chung

2015 ◽  
Vol 16 (4) ◽  
Author(s):  
Svea Petersen ◽  
Christiane Häcker ◽  
Gökmen Turan ◽  
Stefanie Knödler ◽  
Andreas Brodehl ◽  
...  

AbstractFunctionality of implant surfaces is considerably determined by the conformations of immobilized biomolecules adjustable by the applied surface modification approach. Moreover, surface modification of drug eluting systems often needs to be thoroughly optimized with regard to possible drug losses associated with a loss of functionality. Here, we thoroughly investigate biomolecule surface loading, functionality, morphology and stability in dependence of modification conditions at the example of a site-selective immobilization of anti-CD34 antibodies (CD34-Ab) to drug-eluting stents with the aim of providing general information on the biofunctionalization of drug eluting systems. In this context, we demonstrate that the reaction time of biomolecule immobilization defines achievable surface loads but also drug loss. We could moreover show that the used site-selective immobilization procedure elevated the surface functionality considerably in comparison to surfaces modified by random physisorption. Furthermore investigated bionfunctional stability gives important indications for storage conditions of biofunctionalized implants.


1998 ◽  
Vol 10 (6) ◽  
pp. 1510-1520 ◽  
Author(s):  
Melanie S. Sanford ◽  
Paul T. Charles ◽  
Sarah M. Commisso ◽  
Jenna C. Roberts ◽  
David W. Conrad

ChemPhysChem ◽  
2007 ◽  
Vol 8 (10) ◽  
pp. 1469-1472 ◽  
Author(s):  
Eugene W. L. Chan ◽  
Muhammad N. Yousaf

2001 ◽  
Vol 279 (1) ◽  
pp. 68-72 ◽  
Author(s):  
C. M. Niemeyer ◽  
B. Ceyhan ◽  
S. Gao ◽  
L. Chi ◽  
S. Peschel ◽  
...  

2017 ◽  
Vol 5 (30) ◽  
pp. 7637-7643 ◽  
Author(s):  
M. Shaali ◽  
J. G. Woller ◽  
P. G. Johansson ◽  
J. K. Hannestad ◽  
L. de Battice ◽  
...  

Teflon AF acts as a negative e-beam resist, here utilized for immobilization of porphyrin-anchored single DNA molecules on nanopillars, and precise determination of individual label positions.


Langmuir ◽  
2010 ◽  
Vol 26 (8) ◽  
pp. 5323-5328 ◽  
Author(s):  
Feng Tian ◽  
Nan Cheng ◽  
Nicolas Nouvel ◽  
Jin Geng ◽  
Oren A. Scherman

2013 ◽  
Vol 102 (2) ◽  
pp. 345-355 ◽  
Author(s):  
Svea Petersen ◽  
Anne Strohbach ◽  
Raila Busch ◽  
Stephan B. Felix ◽  
Klaus-Peter Schmitz ◽  
...  

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