Isolation and Maintenance of Mouse Epiblast Stem Cells

2010 ◽  
pp. 25-44 ◽  
Author(s):  
Josh G. Chenoweth ◽  
Paul J. Tesar
Keyword(s):  
Stem Cells ◽  
Epiblast Stem Cells

scholarly journals Formative pluripotent stem cells show features of epiblast cells poised for gastrulation

Cell Research ◽  
2021 ◽  
Author(s):  
Xiaoxiao Wang ◽  
Yunlong Xiang ◽  
Yang Yu ◽  
Ran Wang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cells ◽  
Pluripotent Stem Cells ◽  
Primordial Germ Cells ◽  
Embryonic Stem ◽  
Large Set ◽  
Mouse Escs ◽  
Epiblast Stem Cells ◽  
Early Gastrula

AbstractThe pluripotency of mammalian early and late epiblast could be recapitulated by naïve embryonic stem cells (ESCs) and primed epiblast stem cells (EpiSCs), respectively. However, these two states of pluripotency may not be sufficient to reflect the full complexity and developmental potency of the epiblast during mammalian early development. Here we report the establishment of self-renewing formative pluripotent stem cells (fPSCs) which manifest features of epiblast cells poised for gastrulation. fPSCs can be established from different mouse ESCs, pre-/early-gastrula epiblasts and induced PSCs. Similar to pre-/early-gastrula epiblasts, fPSCs show the transcriptomic features of formative pluripotency, which are distinct from naïve ESCs and primed EpiSCs. fPSCs show the unique epigenetic states of E6.5 epiblast, including the super-bivalency of a large set of developmental genes. Just like epiblast cells immediately before gastrulation, fPSCs can efficiently differentiate into three germ layers and primordial germ cells (PGCs) in vitro. Thus, fPSCs highlight the feasibility of using PSCs to explore the development of mammalian epiblast.

Generation of genome-edited mouse epiblast stem cells via a detour through ES cell-chimeras

2016 ◽  
Vol 91 (4-5) ◽  
pp. 119-125 ◽  
Author(s):  
Pierre Osteil ◽  
Joshua Studdert ◽  
Emilie Wilkie ◽  
Nicolas Fossat ◽  
Patrick P.L. Tam
Keyword(s):  
Stem Cells ◽  
Es Cell ◽  
Epiblast Stem Cells

scholarly journals Pig Epiblast Stem Cells Depend on Activin/Nodal Signaling for Pluripotency and Self-Renewal

2010 ◽  
Vol 19 (10) ◽  
pp. 1627-1636 ◽  
Author(s):  
Ramiro Alberio ◽  
Nicola Croxall ◽  
Cinzia Allegrucci
Keyword(s):  
Stem Cells ◽  
Nodal Signaling ◽  
Self Renewal ◽  
Epiblast Stem Cells

scholarly journals Epiblastin A Induces Reprogramming of Epiblast Stem Cells Into Embryonic Stem Cells by Inhibition of Casein Kinase 1

2016 ◽  
Vol 23 (4) ◽  
pp. 494-507 ◽  
Author(s):  
Andrei Ursu ◽  
Damir J. Illich ◽  
Yasushi Takemoto ◽  
Arthur T. Porfetye ◽  
Miao Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Casein Kinase ◽  
Casein Kinase 1 ◽  
Epiblast Stem Cells
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