scholarly journals Three-Dimensional Fluorescence In Situ Hybridization in Mouse Embryos Using Repetitive Probe Sequences

2010 ◽  
pp. 401-408 ◽  
Author(s):  
Walid E. Maalouf ◽  
Tiphaine Aguirre-Lavin ◽  
Laetitia Herzog ◽  
Isabelle Bataillon ◽  
Pascale Debey ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Three Dimensional ◽  
Mouse Embryos

scholarly journals Short NoteOptimalization of Fluorescence in situ Hybridization Conditions in Mare Oocytes and Mouse Embryos

2008 ◽  
Vol 57 (1) ◽  
pp. 49-55
Author(s):  
Monika Bugno ◽  
Zofia Jabłońska ◽  
Ewa Słota
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Mouse Embryos

scholarly journals Distinct contracted conformations of the Tcra/Tcrd locus during Tcra and Tcrd recombination

2010 ◽  
Vol 207 (9) ◽  
pp. 1835-1841 ◽  
Author(s):  
Han-Yu Shih ◽  
Michael S. Krangel
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Three Dimensional ◽  
Double Negative ◽  
Thymocyte Development ◽  
Double Positive ◽  
Long Distance ◽  
Large Pool ◽  
Antigen Receptor Loci

Studies have suggested that antigen receptor loci adopt contracted conformations to promote long-distance interactions between gene segments during V(D)J recombination. The Tcra/Tcrd locus is unique because it undergoes highly divergent Tcrd and Tcra recombination programs in CD4−CD8− double negative (DN) and CD4+CD8+ double positive (DP) thymocytes, respectively. Using three-dimensional fluorescence in situ hybridization, we asked whether these divergent recombination programs are supported by distinct conformational states of the Tcra/Tcrd locus. We found that the 3′ portion of the locus is contracted in DN and DP thymocytes but not in B cells. Remarkably, the 5′ portion of the locus is contracted in DN thymocytes but is decontracted in DP thymocytes. We propose that the fully contracted conformation in DN thymocytes allows Tcrd rearrangements involving Vδ gene segments distributed over 1 Mb, whereas the unique 3′-contracted, 5′-decontracted conformation in DP thymocytes biases initial Tcra rearrangements to the most 3′ of the available Vα gene segments. This would maintain a large pool of distal 5′ Vα gene segments for subsequent rounds of recombination. Thus, distinct contracted conformations of the Tcra/Tcrd locus may facilitate a transition from a Tcrd to a Tcra mode of recombination during thymocyte development.

mRNA fluorescence in situ hybridization to determine overlapping gene expression in whole-mount mouse embryos

2013 ◽  
Vol 242 (9) ◽  
pp. 1094-1100 ◽  
Author(s):  
Stanley J. Neufeld ◽  
Xiaolan Zhou ◽  
Peter D. Vize ◽  
John Cobb
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Mouse Embryos ◽  
Overlapping Gene ◽  
Whole Mount

Localization of Nucleus Organizing Regions (NORs) in One-Cell Mouse Embryos and Oocytes by Fluorescence in situ Hybridization

2004 ◽  
Vol 35 (5) ◽  
pp. 270-278
Author(s):  
F. V. Korobova ◽  
L. G. Romanova ◽  
E. M. Noniashvili ◽  
A. P. Dyban ◽  
O. V. Zatsepina
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Mouse Embryos

Spatial Preservation of Nuclear Chromatin Architecture during Three-Dimensional Fluorescence in Situ Hybridization (3D-FISH)

2002 ◽  
Vol 276 (1) ◽  
pp. 10-23 ◽  
Author(s):  
Irina Solovei ◽  
Antonio Cavallo ◽  
Lothar Schermelleh ◽  
Françoise Jaunin ◽  
Catia Scasselati ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Three Dimensional ◽  
Nuclear Chromatin ◽  
Chromatin Architecture ◽  
3D Fish
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