Characterization of Anti-competitor Activities Produced by Oral Bacteria

Author(s):  
Fengxia Qi ◽  
Jens Kreth
Keyword(s):  
2020 ◽  
Vol 78 (7) ◽  
pp. 541-546 ◽  
Author(s):  
Akiko Oshiro ◽  
Takashi Zaitsu ◽  
Masayuki Ueno ◽  
Yoko Kawaguchi

2000 ◽  
Vol 68 (5) ◽  
pp. 2621-2629 ◽  
Author(s):  
K. Anne Clancy ◽  
Sylvia Pearson ◽  
William H. Bowen ◽  
Robert A. Burne

ABSTRACT Dental caries results from prolonged plaque acidification that leads to the establishment of a cariogenic microflora and demineralization of the tooth. Urease enzymes of oral bacteria hydrolyze urea to ammonia, which can neutralize plaque acids. To begin to examine the relationship between plaque ureolytic activity and the incidence of dental caries, recombinant, ureolytic strains ofStreptococcus mutans were constructed. Specifically, theureABCEFGD operon from Streptococcus salivarius57.I was integrated into the S. mutans chromosome in such a way that the operon was transcribed from a weak, cognate promoter inS. mutans ACUS4 or a stronger promoter in S. mutans ACUS6. Both strains expressed NiCl2-dependent urease activity, but the maximal urease levels in ACUS6 were threefold higher than those in ACUS4. In vitro pH drop experiments demonstrated that the ability of the recombinant S. mutans strains to moderate a decrease in pH during the simultaneous metabolism of glucose and urea increased proportionately with the level of urease activity expressed. Specific-pathogen-free rats that were infected with ACUS6 and fed a cariogenic diet with drinking water containing 25 mM urea and 50 μM NiCl2 had relatively high levels of oral urease activity, as well as dramatic decreases in the prevalence of smooth-surface caries and the severity of sulcal caries, relative to controls. Urease activity appears to influence plaque biochemistry and metabolism in a manner that reduces cariogenicity, suggesting that recombinant, ureolytic bacteria may be useful to promote dental health.


2017 ◽  
Vol 8 (5) ◽  
pp. 1955-1965 ◽  
Author(s):  
C. C. Neto ◽  
K. A. Penndorf ◽  
M. Feldman ◽  
S. Meron-Sudai ◽  
Z. Zakay-Rones ◽  
...  

A mixture of compounds identified in non-dialyzable fraction from cranberry juice inhibits adhesion, coaggregation and biofilm formation by oral bacteria.


Author(s):  
H. Ranta ◽  
M. Haapasalo ◽  
E. Kerosuo ◽  
K. Lounatmaa
Keyword(s):  

1987 ◽  
Vol 55 (5) ◽  
pp. 1198-1202 ◽  
Author(s):  
E I Weiss ◽  
J London ◽  
P E Kolenbrander ◽  
A S Kagermeier ◽  
R N Andersen
Keyword(s):  

2017 ◽  
Vol 136 ◽  
pp. 21-29 ◽  
Author(s):  
Daniel K.Y. Solaiman ◽  
Richard D. Ashby ◽  
Joseph Uknalis

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