Ribosome Display for the Selection of Sac7d Scaffolds

Author(s):  
Barbara Mouratou ◽  
Ghislaine Béhar ◽  
Lauranne Paillard-Laurance ◽  
Stéphane Colinet ◽  
Frédéric Pecorari
2004 ◽  
Vol 284 (1-2) ◽  
pp. 147-157 ◽  
Author(s):  
Myung-Shin Lee ◽  
Myung-Hee Kwon ◽  
Kyongmin Hwang Kim ◽  
Ho-Joon Shin ◽  
Sun Park ◽  
...  

2001 ◽  
Vol 254 (1-2) ◽  
pp. 191-197 ◽  
Author(s):  
Gregory Coia ◽  
Luisa Pontes-Braz ◽  
Stewart D Nuttall ◽  
Peter J Hudson ◽  
Robert A Irving

2012 ◽  
Vol 168 (5) ◽  
pp. 967-979 ◽  
Author(s):  
Yangbin Pan ◽  
Weiping Mao ◽  
Xuanxuan Liu ◽  
Chong Xu ◽  
Zhijuan He ◽  
...  

2018 ◽  
Author(s):  
Adinarayana Kunamneni ◽  
Elizabeth C. Clarke ◽  
Chunyan Ye ◽  
Steven B. Bradfute ◽  
Ravi Durvasula

AbstractFiloviruses, which include ebolaviruses and marburgvirus, can cause outbreaks of highly lethal hemorrhagic fever. This disease causes significant morbidity and mortality in humans and non-human primates, with human fatality rates reaching 90% during some outbreaks. Currently, there are a lack of licensed vaccines or antivirals for these viruses. Since early symptoms of filovirus infection mimic more common diseases, there is a strong unmet public health and biodefense need for broad-spectrum filovirus rapid diagnostics. We have generated a panel of mouse single-chain Fv-antibodies (scFvs) to filovirus glycoproteins (GPs) using cell-free ribosome display and determined their cross-reactivity profiles to all known filovirus species. Two scFvs (4-2 and 22-1) were able to detect all known Ebolavirus and Marburgvirus species. This is the first report on ribosome display scFvs that can detect a broad set of filovirus GPs, which demonstrates their potential use in the development of a new generation of rapid diagnostic immunoassays.


Author(s):  
Valentina Kalichuk ◽  
Stanimir Kambarev ◽  
Ghislaine Béhar ◽  
Benjamin Chalopin ◽  
Axelle Renodon-Cornière ◽  
...  

2018 ◽  
Vol 109 (5) ◽  
pp. 1503-1512
Author(s):  
Shangke Huang ◽  
Lu Feng ◽  
Gaili An ◽  
Xiaojin Zhang ◽  
Zixuan Zhao ◽  
...  

2010 ◽  
Vol 17 (5) ◽  
pp. 585-590 ◽  
Author(s):  
Fang Li ◽  
Pingping Su ◽  
Chao Lin ◽  
Hong Li ◽  
Jiajing Cheng ◽  
...  

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