Zinc-Finger Nucleases-Based Genome Engineering to Generate Isogenic Human Cell Lines

2011 ◽  
pp. 145-156 ◽  
Author(s):  
Anne-Kathrin Dreyer ◽  
Toni Cathomen
Keyword(s):  
Cell Lines ◽  
Zinc Finger ◽  
Human Cell ◽  
Genome Engineering ◽  
Zinc Finger Nucleases ◽  
Human Cell Lines

scholarly journals Isogenic Human Cell Lines for Drug Discovery: Regulation of Target Gene Expression by Engineered Zinc-Finger Protein Transcription Factors

2005 ◽  
Vol 10 (4) ◽  
pp. 304-313 ◽  
Author(s):  
Pei-Qi Liu ◽  
Siyuan Tan ◽  
Matthew C. Mendel ◽  
Richard J. Murrills ◽  
Bheem M. Bhat ◽  
...  
Keyword(s):  
Cell Lines ◽  
Zinc Finger ◽  
Human Cell ◽  
Target Gene ◽  
Zinc Finger Protein ◽  
Cyclic Adenosine Monophosphate ◽  
Adenosine Monophosphate ◽  
Hek293 Cells ◽  
Human Cell Lines ◽  
Finger Protein

Isogenic cell lines differing only in the expression of the protein of interest provide the ideal platform for cell-based screening. However, related natural lines differentially expressing the therapeutic target of choice are rare. Here the authors report a strategy for drug screening employing isogenic human cell lines in which the expression of the target protein is regulated by a gene-specific engineered zinc-finger protein (ZFP) transcription factor (TF). To demonstrate this approach, a ZFP TF activator of the human parathyroid hormone receptor 1 (PTHR1) gene was identified and introduced into HEK293 cells (negative for PTHR1). Following induction of ZFP TF expression, this cell line produced functional PTHR1 protein, resulting in a robust and ligand-specific cyclic adenosine monophosphate (cAMP) response. Reciprocally, the natural expression of PTHR1 observed in SAOS2 cells was dramatically reduced by the introduction of the appropriate PTHR1-specific ZFP TF repressor. Moreover, this ZFP-driven PTHR1 repression selectively eliminated the functional cAMP response invoked by known ligands of PTHR1. These data establish ZFP TF–generated isogenic lines as a general approach for the identification of therapeutic agents specific for the target gene of interest.

A zinc finger nuclease assay to rapidly quantitate homologous recombination proficiency in human cell lines

2013 ◽  
Vol 434 (1) ◽  
pp. 96-98 ◽  
Author(s):  
Jing Yuan ◽  
Peter R. Strack ◽  
Carlo Toniatti ◽  
Marc Pelletier
Keyword(s):  
Homologous Recombination ◽  
Cell Lines ◽  
Zinc Finger ◽  
Human Cell ◽  
Zinc Finger Nuclease ◽  
Human Cell Lines ◽  
Nuclease Assay

Investigations of the influence of microgravity on the state of human cell lines

2004 ◽  
Vol 10 (5-6) ◽  
pp. 226-228
Author(s):  
L.M. Nosach ◽  
◽  
O.Yu. Povnitsa ◽  
V.L. Zhovnovata ◽  
◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
The State ◽  
Human Cell Lines
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