Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal: A Review

2020 ◽  
pp. 607-624
Author(s):  
Ganeshan Sivanandhan ◽  
Natesan Selvaraj ◽  
Andy Ganapathi ◽  
Yong Pyo Lim
Keyword(s):  
Hairy Root ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture

scholarly journals An efficient hairy root culture system for Withania somnifera (L.) Dunal

2014 ◽  
Vol 13 (43) ◽  
pp. 4141-4147 ◽  
Author(s):  
Sivanandhan Ganeshan ◽  
Selvaraj Natesan ◽  
Ganapathi Andy ◽  
Manickavasagam Markandan
Keyword(s):  
Hairy Root ◽  
Culture System ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture

scholarly journals Optimization of Carbon Source for Hairy Root Growth and Withaferin A and Withanone Production in Withania somnifera

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Ganeshan Sivanandhan ◽  
Manoharan Rajesh ◽  
Muthukrishnan Arun ◽  
Murugaraj Jeyaraj ◽  
Gnanajothi Kapil Dev ◽  
...  
Keyword(s):  
Carbon Source ◽  
Liquid Medium ◽  
Hairy Root ◽  
Root Biomass ◽  
Withania Somnifera ◽  
Carbon Sources ◽  
Root Culture ◽  
Biomass Accumulation ◽  
Hairy Root Culture ◽  
Withaferin A

This study optimized carbon sources in half MS liquid medium for maximum biomass accumulation and withanolides production in hairy root culture of Withania somnifera. The highest production of withaferin A and withanone was achieved when sucrose and sucrose+glucose were used individually as carbon sources. The hairy root suspension culture supplemented with a lower level of sucrose (2%) favored hairy root biomass accumulation (1.41 g DW) followed by sucrose+glucose (2+1) when compared with other carbon sources in half MS liquid medium after 40 days of culture. The hairy roots grown on sucrose (4%) enriched half MS liquid medium stimulated higher production of withaferin A (2.21 mg/g DW) and withanone (2.41 mg/g DW) on the 40th day of culture, followed by sucrose+glucose (4+1%) compared with glucose, fructose, maltose and other combinations tested.

Expression of important pathway genes involved in withanolides biosynthesis in hairy root culture of Withania somnifera upon treatment with Gracilaria edulis and Sargassum wightii

2015 ◽  
Vol 91 ◽  
pp. 61-64 ◽  
Author(s):  
Ganeshan Sivanandhan ◽  
Chinnathambi Arunachalam ◽  
Natesan Selvaraj ◽  
Ali Alharbi Sulaiman ◽  
Yong Pyo Lim ◽  
...  
Keyword(s):  
Hairy Root ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Gracilaria Edulis ◽  
Sargassum Wightii ◽  
Important Pathway

Up-regulation of Squalene synthase in hairy root culture of Withania somnifera (L.) Dunal yields higher quantities of withanolides

2020 ◽  
Vol 154 ◽  
pp. 112706
Author(s):  
Ganeshan Sivanandhan ◽  
Natesan Selvaraj ◽  
Andy Ganapathi ◽  
Yong Pyo Lim
Keyword(s):  
Hairy Root ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Squalene Synthase

scholarly journals Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment

3 Biotech ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 949-956 ◽  
Author(s):  
Chandrasekaran Thilip ◽  
Chellappan Soundar Raju ◽  
Kandhan Varutharaju ◽  
Abubakker Aslam ◽  
Appakan Shajahan
Keyword(s):  
Heat Treatment ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Culture System ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture

Elicitation of withaferin-A in hairy root culture of Withania somnifera (L.) Dunal using natural polysaccharides

Biologia ◽  
2019 ◽  
Vol 74 (8) ◽  
pp. 961-968 ◽  
Author(s):  
Chandrasekaran Thilip ◽  
Valiyaparambath Musfir Mehaboob ◽  
Kandhan Varutharaju ◽  
Kunnampalli Faizal ◽  
Palusamy Raja ◽  
...  
Keyword(s):  
Hairy Root ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Withaferin A
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