scholarly journals Optimization of Carbon Source for Hairy Root Growth and Withaferin A and Withanone Production in Withania somnifera

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Ganeshan Sivanandhan ◽  
Manoharan Rajesh ◽  
Muthukrishnan Arun ◽  
Murugaraj Jeyaraj ◽  
Gnanajothi Kapil Dev ◽  
...  
Keyword(s):  
Carbon Source ◽  
Liquid Medium ◽  
Hairy Root ◽  
Root Biomass ◽  
Withania Somnifera ◽  
Carbon Sources ◽  
Root Culture ◽  
Biomass Accumulation ◽  
Hairy Root Culture ◽  
Withaferin A

This study optimized carbon sources in half MS liquid medium for maximum biomass accumulation and withanolides production in hairy root culture of Withania somnifera. The highest production of withaferin A and withanone was achieved when sucrose and sucrose+glucose were used individually as carbon sources. The hairy root suspension culture supplemented with a lower level of sucrose (2%) favored hairy root biomass accumulation (1.41 g DW) followed by sucrose+glucose (2+1) when compared with other carbon sources in half MS liquid medium after 40 days of culture. The hairy roots grown on sucrose (4%) enriched half MS liquid medium stimulated higher production of withaferin A (2.21 mg/g DW) and withanone (2.41 mg/g DW) on the 40th day of culture, followed by sucrose+glucose (4+1%) compared with glucose, fructose, maltose and other combinations tested.

Elicitation of withaferin-A in hairy root culture of Withania somnifera (L.) Dunal using natural polysaccharides

Biologia ◽  
2019 ◽  
Vol 74 (8) ◽  
pp. 961-968 ◽  
Author(s):  
Chandrasekaran Thilip ◽  
Valiyaparambath Musfir Mehaboob ◽  
Kandhan Varutharaju ◽  
Kunnampalli Faizal ◽  
Palusamy Raja ◽  
...  
Keyword(s):  
Hairy Root ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Withaferin A

Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal: A Review

2020 ◽  
pp. 607-624
Author(s):  
Ganeshan Sivanandhan ◽  
Natesan Selvaraj ◽  
Andy Ganapathi ◽  
Yong Pyo Lim
Keyword(s):  
Hairy Root ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture

scholarly journals An efficient hairy root culture system for Withania somnifera (L.) Dunal

2014 ◽  
Vol 13 (43) ◽  
pp. 4141-4147 ◽  
Author(s):  
Sivanandhan Ganeshan ◽  
Selvaraj Natesan ◽  
Ganapathi Andy ◽  
Manickavasagam Markandan
Keyword(s):  
Hairy Root ◽  
Culture System ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture

Diterpenoid Production in Hairy Root Culture of Salvia sclarea L.

2008 ◽  
Vol 63 (7-8) ◽  
pp. 621-624 ◽  
Author(s):  
Łukasz Kuzma ◽  
Elzbieta Bruchajze ◽  
Halina Wysokińska
Keyword(s):  
Liquid Medium ◽  
Hairy Root ◽  
Total Content ◽  
Root Culture ◽  
Dry Weight ◽  
Hairy Root Culture ◽  
Growth Cycle ◽  
Transformed Roots ◽  
Salvia Sclarea ◽  
Half Strength

Growth and diterpenoid accumulation (salvipisone, ferruginol, aethiopinone and 1-oxoaethiopinone) during the growth cycle of a Salvia sclarea hairy root culture are described. The roots transformed by Agrobacterium rhizogenes (LBA 9402) were cultured in half-strength B5 liquid medium supplemented with 30 g L-1 sucrose under light (16 h/8 h light/dark). A culture period of 30 days was optimal for both biomass and diterpenoid production. The total content of four diterpenoids in the hairy roots [(27.3 ± 0.6) mg g-1 dry weight] was higher than that of roots of field-grown S. sclarea plants [(3.15 ± 0.15) mg g-1 dry weight]. In transformed roots, aethiopinone was the main diterpenoid, whereas the principal diterpenoid of natural roots was salvipisone.

scholarly journals Effect of Carbon Source on Biomass and Flavonoid Content of Gynuraprocumbens (Lour.) Merr Adventitious Root in Liquid Culture

2019 ◽  
Vol 16 (1) ◽  
pp. 121-127
Author(s):  
Layin Muthoharoh ◽  
Hanik Faizah ◽  
Popy Hartatie Hardjo ◽  
Alfinda Novi Kristanti ◽  
Yosephine Sri Wulan Manuhara
Keyword(s):  
Carbon Source ◽  
Liquid Medium ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Carbon Sources ◽  
Leaf Explants ◽  
Root Culture ◽  
Ms Medium ◽  
Flavonoid Content ◽  
Initial Inoculum

Gynura procumbens (Lour) Merr is a medicinal plant that has been widely used as an ingredient of herbal medicine. G. procumbens is known to contains secondary metabolite compounds namely flavonoids. The aim of this study was to investigate the effect of carbon sources on biomass and flavonoid content of G. procumbens adventitious root culture grown in agitated liquid medium. Adventitious roots were induced from leaf explants on Murashige and Skoog (MS) medium supplemented with 3% sucrose and 8 g/Lagar. G. procumbens adventitious root cultures were performed in MS liquid medium containing different carbon source of 3% sucrose, 5% sucrose, 3% glucose and 4% fructose, and supplemented with5 mg/L IBA. Two grams of adventitious roots as the initial inoculum were cultured in1000 mL Erlenmeyer flask containing 250 mL MS medium and agitated at 70 rpm in dark conditions for 28 days.The results showed that medium with the addition of 5% sucrose produced the highest fresh weight (10.23 ± 0.86 g) followed by 3% sucrose (8.39 ± 0.60 g), while the addition of 3% glucose (3.35 ± 0.11 g) and 4% fructose (3.68 ± 1.13 g) produced adventitious root biomass lower compared to the addition of sucrose.The production of G. procumbens adventitious roots biomass increased 5-fold of the initial inoculum. The highest flavonoid content was obtained in MS medium supplemented with 3% glucose with the content of kaempferol and quercetin reached 25.44g/L per 1gdry weight and 7.08 g/L per gdry weight, respectively.

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