Production of pharmaceutical active recombinant globular adiponectin as a secretory protein in Withania Somnifera hairy root culture

2020 ◽  
Vol 323 ◽  
pp. 302-312
Author(s):  
Sayed Mehdi Dehdashti ◽  
Sumita Acharjee ◽  
Alireza Nomani ◽  
Manab Deka
Keyword(s):  
Hairy Root ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Secretory Protein ◽  
Globular Adiponectin

Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal: A Review

2020 ◽  
pp. 607-624
Author(s):  
Ganeshan Sivanandhan ◽  
Natesan Selvaraj ◽  
Andy Ganapathi ◽  
Yong Pyo Lim
Keyword(s):  
Hairy Root ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture

scholarly journals An efficient hairy root culture system for Withania somnifera (L.) Dunal

2014 ◽  
Vol 13 (43) ◽  
pp. 4141-4147 ◽  
Author(s):  
Sivanandhan Ganeshan ◽  
Selvaraj Natesan ◽  
Ganapathi Andy ◽  
Manickavasagam Markandan
Keyword(s):  
Hairy Root ◽  
Culture System ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture

scholarly journals Optimization of Carbon Source for Hairy Root Growth and Withaferin A and Withanone Production in Withania somnifera

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Ganeshan Sivanandhan ◽  
Manoharan Rajesh ◽  
Muthukrishnan Arun ◽  
Murugaraj Jeyaraj ◽  
Gnanajothi Kapil Dev ◽  
...  
Keyword(s):  
Carbon Source ◽  
Liquid Medium ◽  
Hairy Root ◽  
Root Biomass ◽  
Withania Somnifera ◽  
Carbon Sources ◽  
Root Culture ◽  
Biomass Accumulation ◽  
Hairy Root Culture ◽  
Withaferin A

This study optimized carbon sources in half MS liquid medium for maximum biomass accumulation and withanolides production in hairy root culture of Withania somnifera. The highest production of withaferin A and withanone was achieved when sucrose and sucrose+glucose were used individually as carbon sources. The hairy root suspension culture supplemented with a lower level of sucrose (2%) favored hairy root biomass accumulation (1.41 g DW) followed by sucrose+glucose (2+1) when compared with other carbon sources in half MS liquid medium after 40 days of culture. The hairy roots grown on sucrose (4%) enriched half MS liquid medium stimulated higher production of withaferin A (2.21 mg/g DW) and withanone (2.41 mg/g DW) on the 40th day of culture, followed by sucrose+glucose (4+1%) compared with glucose, fructose, maltose and other combinations tested.

scholarly journals Improved Agrobacterium rhizogenes-mediated hairy root culture system of Withania somnifera (L.) Dunal using sonication and heat treatment

3 Biotech ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 949-956 ◽  
Author(s):  
Chandrasekaran Thilip ◽  
Chellappan Soundar Raju ◽  
Kandhan Varutharaju ◽  
Abubakker Aslam ◽  
Appakan Shajahan
Keyword(s):  
Heat Treatment ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Culture System ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture

Elicitation of withaferin-A in hairy root culture of Withania somnifera (L.) Dunal using natural polysaccharides

Biologia ◽  
2019 ◽  
Vol 74 (8) ◽  
pp. 961-968 ◽  
Author(s):  
Chandrasekaran Thilip ◽  
Valiyaparambath Musfir Mehaboob ◽  
Kandhan Varutharaju ◽  
Kunnampalli Faizal ◽  
Palusamy Raja ◽  
...  
Keyword(s):  
Hairy Root ◽  
Withania Somnifera ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Withaferin A
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