Molecular Weight Determination of DNA-Binding Proteins by UV Cross-linking Combined with SDS Polyacrylamide Gel Electrophoresis

1991 ◽  
pp. 79-91 ◽  
Author(s):  
Melya J. Hughes ◽  
Haimin Liang ◽  
Jean-Pierre Jost
Keyword(s):  
Molecular Weight ◽  
Dna Binding ◽  
Gel Electrophoresis ◽  
Binding Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Dna Binding Proteins ◽  
Polyacrylamide Gel ◽  
Cross Linking ◽  
Molecular Weight Determination

scholarly journals Molecular weight estimation of esterase isoenzymes in closely related Drosophila Species (Diptera: Drosophilidae) in non-denaturing polyacrylamide gel electrophoresis

2009 ◽  
Vol 52 (5) ◽  
pp. 1083-1089 ◽  
Author(s):  
Rogério Pincela Mateus ◽  
Hamilton Cabral ◽  
Gustavo Orlando Bonilla-Rodriguez ◽  
Carlos Roberto Ceron
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Duplication Event ◽  
Polyacrylamide Gel ◽  
Molecular Weight Determination ◽  
Structural Genes ◽  
Repleta Group ◽  
Native Polyacrylamide Gel Electrophoresis

A method that allows the measure of molecular weight of two well-known and closely related esterases from Drosophila mojavensis and its sibling species, D. arizonae, is here described, using native polyacrylamide gel electrophoresis at several concentrations, applying Fergunson´s principles. These enzymes, namely EST-4 and EST-5, presented molecular weight values between 81 and 91 kDa. In spite of their distinct expression pattern through the insect's life cycle, they showed properties of isoenzymes codified by distinct structural genes, supporting the hypothesis of a rather recent gene duplication event that generated both in D. mojavensis and D. arizonae, as well as in other species of repleta group. The method is simple and adequate to be applied to preliminary molecular weight determination of other enzymes without any previous purification procedure.

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