Agrobacterium, The Genetic Engineer

Author(s):  
Paul J. J. Hooykaas
Keyword(s):  
1987 ◽  
Vol 9 (4) ◽  
pp. 250-251 ◽  
Author(s):  
Peter G. Sanders
Keyword(s):  

Science ◽  
2001 ◽  
Vol 294 (5550) ◽  
pp. 2245f-2245
Keyword(s):  

2018 ◽  
Vol 3 (2) ◽  
pp. 97-101
Author(s):  
Yohanes Cacur ◽  
Jonshon Tarigan ◽  
Bartholomeus Pasangka

Abstrak Telah dilakukan penelitian dengan menggunakan metode radiasi multigamma untuk rekayasa genetik pada tanamn sorgum lokal asal Niki-Niki Soe di Desa Penfui, Kecamatan Kupang Tengah, Kabupaten Kupang. Penelitian ini bertujuan untuk menentukan dosis yang tepat untuk pengembangan sorgum lokal dengan produksi tinggi dan menentukan karateristik bakal unggul tanaman sorgum yang diradiasi sumber multigamma serta mengestimasi peningkatan produksi tanaman sorgum yang dikembangkan dengan iradiasi multigamma. Karateristik bakal unggul yang lebih baik pada dosis 3000 rads dibandingkan dengan 4000 rads dan 2000 rads. Estimasi peningkatan sorgum lokal ditunjukkan pada dosis 3000 rads meningkat 16.83% berbeda dibandingkan dengan dosis 4000 rads dan 2000 rads masing-masing mencapai 9.02% dan 2.33%. Kata kunci: pemulian, sorgum, radiasi, multigamma Abstract THE USE OF MULTIGAMMA RADIATION FOR GENETIC ENGINEER LOCAL SORGUM PLANTS FROM NIKI-NIKI SOE A research has been done to use multigamma radiaton methods for genetic engineer to local sorgum plants from Niki-Niki Soe in Desa Penfui, Subdistrict Kupang Tengah, Regency Kupang. The purpose of this research is to determine the right dose for the development of local sorgum with high production and to determine the characteristic of the excellent shoot of sorgum plants that radiated by multigamma source also to estimate upgrading production of sorgum plants that have been develop by multigamma radiation. The result of the research shows that the right dose for the preservation of sorgum plants are range around 3000 rads and 4000 rads. The upgrading estimation on local sorgum was shown on the dose of 3000 tads which increased 20,2% differ with the dose of 4000 rads and 2000 rads which each of it increased about 9.92% and 2.38%. Keywords: Preservation, Sorgum, Radiation, Multigamma


2003 ◽  
Vol 67 (1) ◽  
pp. 16-37 ◽  
Author(s):  
Stanton B. Gelvin

SUMMARY Agrobacterium tumefaciens and related Agrobacterium species have been known as plant pathogens since the beginning of the 20th century. However, only in the past two decades has the ability of Agrobacterium to transfer DNA to plant cells been harnessed for the purposes of plant genetic engineering. Since the initial reports in the early 1980s using Agrobacterium to generate transgenic plants, scientists have attempted to improve this “natural genetic engineer” for biotechnology purposes. Some of these modifications have resulted in extending the host range of the bacterium to economically important crop species. However, in most instances, major improvements involved alterations in plant tissue culture transformation and regeneration conditions rather than manipulation of bacterial or host genes. Agrobacterium-mediated plant transformation is a highly complex and evolved process involving genetic determinants of both the bacterium and the host plant cell. In this article, I review some of the basic biology concerned with Agrobacterium-mediated genetic transformation. Knowledge of fundamental biological principles embracing both the host and the pathogen have been and will continue to be key to extending the utility of Agrobacterium for genetic engineering purposes.


Science ◽  
1979 ◽  
Vol 203 (4377) ◽  
pp. 254-255 ◽  
Author(s):  
J. L. MARX

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