Molecular Typing of Lung Adenocarcinoma on Cytological Samples in the Next-Generation Sequencing Era

2015 ◽  
pp. 367-379
Author(s):  
Rocco Cappellesso ◽  
Ambrogio Fassina ◽  
Emilio Bria ◽  
Aldo Scarpa ◽  
Matteo Fassan
Keyword(s):  
Next Generation Sequencing ◽  
Lung Adenocarcinoma ◽  
Molecular Typing ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Molecular Typing of Lung Adenocarcinoma on Cytological Samples Using a Multigene Next Generation Sequencing Panel

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e80478 ◽  
Author(s):  
Aldo Scarpa ◽  
Katarzyna Sikora ◽  
Matteo Fassan ◽  
Anna Maria Rachiglio ◽  
Rocco Cappellesso ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Lung Adenocarcinoma ◽  
Molecular Typing ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Mutation Profile of Resected EGFR ‐Mutated Lung Adenocarcinoma by Next‐Generation Sequencing

2019 ◽  
Vol 24 (10) ◽  
pp. 1368-1374 ◽  
Author(s):  
Ze‐Rui Zhao ◽  
Yao‐Bin Lin ◽  
Calvin S.H. Ng ◽  
Rong Zhang ◽  
Xue Wu ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Lung Adenocarcinoma ◽  
Next Generation ◽  
Mutation Profile ◽  
Egfr Mutated ◽  
Generation Sequencing

scholarly journals Next-Generation Sequencing Approach to Non–Small Cell Lung Carcinoma Yields More Actionable Alterations

2017 ◽  
Vol 142 (3) ◽  
pp. 353-357 ◽  
Author(s):  
Mitra Mehrad ◽  
Somak Roy ◽  
Humberto Trejo Bittar ◽  
Sanja Dacic
Keyword(s):  
Next Generation Sequencing ◽  
Lung Adenocarcinoma ◽  
Small Cell ◽  
Gene Panel ◽  
Next Generation ◽  
Small Cell Lung ◽  
Genomic Alterations ◽  
Generation Sequencing ◽  
Gene Alterations ◽  
Cancer Panel

Context.— Different testing algorithms and platforms for EGFR mutations and ALK rearrangements in advanced-stage lung adenocarcinoma exist. The multistep approach with single-gene assays has been challenged by more efficient next-generation sequencing (NGS) of a large number of gene alterations. The main criticism of the NGS approach is the detection of genomic alterations of uncertain significance. Objective.— To determine the best testing algorithm for patients with lung cancer in our clinical practice. Design.— Two testing approaches for metastatic lung adenocarcinoma were offered between 2012–2015. One approach was reflex testing for an 8-gene panel composed of DNA Sanger sequencing for EGFR, KRAS, PIK3CA, and BRAF and fluorescence in situ hybridization for ALK, ROS1, MET, and RET. At the oncologist's request, a subset of tumors tested by the 8-gene panel was subjected to a 50-gene Ion AmpliSeq Cancer Panel. Results.— Of 1200 non–small cell lung carcinomas (NSCLCs), 57 including 46 adenocarcinomas and NSCLCs, not otherwise specified; 7 squamous cell carcinomas (SCCs); and 4 large cell neuroendocrine carcinomas (LCNECs) were subjected to Ion AmpliSeq Cancer Panel. Ion AmpliSeq Cancer Panel detected 9 potentially actionable variants in 29 adenocarcinomas that were wild type by the 8-gene panel testing (9 of 29, 31.0%) in the following genes: ERBB2 (3 of 29, 10.3%), STK11 (2 of 29, 6.8%), PTEN (2 of 29, 6.8%), FBXW7 (1 of 29, 3.4%), and BRAF G469A (1 of 29, 3.4%). Four SCCs and 2 LCNECs showed investigational genomic alterations. Conclusions.— The NGS approach would result in the identification of a significant number of actionable gene alterations, increasing the therapeutic options for patients with advanced NSCLCs.

VPS41-BRAF, a novel BRAF fusion gene identified in a lung adenocarcinoma patient by next-generation sequencing

Lung Cancer ◽  
2020 ◽  
Vol 146 ◽  
pp. 380-381
Author(s):  
Lujuan Wang ◽  
Anqi Chen ◽  
Xiaoling She ◽  
Juanjuan Xiang ◽  
Jingqun Tang
Keyword(s):  
Next Generation Sequencing ◽  
Lung Adenocarcinoma ◽  
Fusion Gene ◽  
Next Generation ◽  
Lung Adenocarcinoma Patient ◽  
Braf Fusion ◽  
Generation Sequencing

scholarly journals Molecular characterization of lung adenocarcinoma from Korean patients using next generation sequencing

PLoS ONE ◽  
2019 ◽  
Vol 14 (11) ◽  
pp. e0224379 ◽  
Author(s):  
You Jin Chun ◽  
Jae Woo Choi ◽  
Min Hee Hong ◽  
Dongmin Jung ◽  
Hyeonju Son ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Lung Adenocarcinoma ◽  
Molecular Characterization ◽  
Next Generation ◽  
Korean Patients ◽  
Generation Sequencing

scholarly journals P3.02-009 Mutation Detection in Cell-Free DNA from Patients with Lung Adenocarcinoma by Next-Generation Sequencing

2017 ◽  
Vol 12 (11) ◽  
pp. S2240-S2241
Author(s):  
H. Faisal ◽  
S. Hirano ◽  
F. Irisuna ◽  
S. Kimura ◽  
N. Morihara ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Lung Adenocarcinoma ◽  
Mutation Detection ◽  
Next Generation ◽  
Cell Free Dna ◽  
Free Dna ◽  
Generation Sequencing
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