Standardized Tape Stripping: A Practical and Reproducible Protocol to Reduce Uniformly the Stratum Corneum

2015 ◽  
pp. 1-9
Author(s):  
Heinrich Dickel ◽  
Alexandros Goulioumis ◽  
Thilo Gambichler ◽  
Joachim Fluhr ◽  
Jeanette Kamphowe ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Tape Stripping

Tape Stripping Method and Stratum Corneum

2004 ◽  
pp. 531-547 ◽  
Author(s):  
Hongbo Zhai ◽  
Howard Maibach ◽  
Myeong Jun Choi
Keyword(s):  
Stratum Corneum ◽  
Tape Stripping ◽  
Stripping Method

scholarly journals Evaluation of the Stratum Corneum Exfoliated by Tape Stripping Method (No. 1)

1997 ◽  
Vol 32 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Masayuki Matsumoto ◽  
Shoji Hayashi ◽  
Seiichi Arai
Keyword(s):  
Stratum Corneum ◽  
Tape Stripping ◽  
Stripping Method

scholarly journals Stratum Corneum Sampling to Assess Bioequivalence between Topical Acyclovir Products

2019 ◽  
Vol 36 (12) ◽  
Author(s):  
A. Pensado ◽  
W.S. Chiu ◽  
S. F. Cordery ◽  
E. Rantou ◽  
A. L. Bunge ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Statistical Power ◽  
Application Site ◽  
Tape Stripping ◽  
Local Skin ◽  
Drug Products ◽  
Single Time Point ◽  
Topical Acyclovir ◽  
Alternative Metrics

Abstract Purpose To examine the potential of stratum corneum (SC) sampling via tape-stripping in humans to assess bioequivalence of topical acyclovir drug products, and to explore the potential value of alternative metrics of local skin bioavailability calculable from SC sampling experiments. Methods Three acyclovir creams were considered in two separate studies in which drug amounts in the SC after uptake and clearance periods were measured and used to assess bioequivalence. In each study, a “reference” formulation (evaluated twice) was compared to the “test” in 10 subjects. Each application site was replicated to achieve greater statistical power with fewer volunteers. Results SC sampling revealed similarities and differences between products consistent with results from other surrogate bioequivalence measures, including dermal open-flow microperfusion experiments. Further analysis of the tape-stripping data permitted acyclovir flux into the viable skin to be deduced and drug concentration in that ‘compartment’ to be estimated. Conclusions Acyclovir quantities determined in the SC, following a single-time point uptake and clearance protocol, can be judiciously used both to objectively compare product performance in vivo and to assess delivery of the active into skin tissue below the barrier, thereby permitting local concentrations at or near to the site of action to be determined.

Biology of Stratum Corneum: Tape Stripping and Protein Quantification

2016 ◽  
pp. 387-396
Author(s):  
Hanjiang Zhu ◽  
Ali Alikhan ◽  
Howard I. Maibach
Keyword(s):  
Stratum Corneum ◽  
Protein Quantification ◽  
Tape Stripping

scholarly journals Side-by-Side Comparison of Skin Biopsies and Skin Tape Stripping Highlights Abnormal Stratum Corneum in Atopic Dermatitis

2019 ◽  
Vol 139 (11) ◽  
pp. 2387-2389.e1 ◽  
Author(s):  
Byung Eui Kim ◽  
Elena Goleva ◽  
Peter S. Kim ◽  
Kathryn Norquest ◽  
Caroline Bronchick ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Tape Stripping ◽  
Skin Biopsies

scholarly journals Tape stripping method is useful for the quantification of antimicrobial peptides on the human skin surface including the stratum corneum

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shigeyuki Ono ◽  
Nobuhiko Eda ◽  
Takuya Mori ◽  
Atsuko Otsuka ◽  
Nobuhiro Nakamura ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Stratum Corneum ◽  
Human Skin ◽  
Significant Positive Correlation ◽  
Skin Surface ◽  
Tape Stripping ◽  
Established Method ◽  
Positive Correlation ◽  
Physiological Importance ◽  
Stripping Method

Abstract Antimicrobial peptides (AMPs) play an important role in innate immunity in human skin. It is known that AMPs mainly function in the stratum corneum. Therefore, AMP concentrations in the stratum corneum need to be precisely measured to clarify functional and physiological importance of AMPs in cutaneous defence. Tape stripping (TS) is a well-established method by which components in the stratum corneum can be collected. However, the usefulness of the TS method for measuring AMP concentration in human skin remains unclear. Therefore, we compared it with another popular method, skin rinsing, which had been established as a method for measuring AMP concentration in human skin. When investigated on healthy medial forearm using RNase 7, which is one of the typical AMPs, as an index, there was a significant positive correlation between RNase 7 concentrations measured by the TS method at adjacent forearm sites, demonstrating the reproducibility of the TS method. Next, a significant positive correlation was detected in RNase 7 concentrations measured using the TS and the skin rinsing method, indicating that the TS method is comparable to the skin rinsing method. Thus, we speculate that the TS method is useful for measuring AMP concentration in human skin.

scholarly journals Solvent effects on the harvesting of stratum corneum from hairless mouse skin through adhesive tape stripping in vitro

1991 ◽  
Vol 68 (1-3) ◽  
pp. 127-133 ◽  
Author(s):  
Tsai Jui-Chen ◽  
Markus J. Cappel ◽  
Norman D. Weiner ◽  
Gordon L. Flynn ◽  
James Ferry
Keyword(s):  
Stratum Corneum ◽  
Solvent Effects ◽  
Mouse Skin ◽  
Hairless Mouse ◽  
Tape Stripping ◽  
Adhesive Tape ◽  
Hairless Mouse Skin
Sign in / Sign up

Export Citation Format

Share Document