scholarly journals Side-by-Side Comparison of Skin Biopsies and Skin Tape Stripping Highlights Abnormal Stratum Corneum in Atopic Dermatitis

2019 ◽  
Vol 139 (11) ◽  
pp. 2387-2389.e1 ◽  
Author(s):  
Byung Eui Kim ◽  
Elena Goleva ◽  
Peter S. Kim ◽  
Kathryn Norquest ◽  
Caroline Bronchick ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Tape Stripping ◽  
Skin Biopsies

Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis

Dermatology ◽  
2021 ◽  
pp. 1-6
Author(s):  
Stine Simonsen ◽  
Peter Brøgger ◽  
Sanja Kezic ◽  
Jacob P. Thyssen ◽  
Lone Skov
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Tape Stripping ◽  
Mrna Levels ◽  
Protein Levels ◽  
Cytokine Levels ◽  
Invasive Method ◽  
Healthy Control ◽  
Skin Biopsies ◽  
Control Skin

Skin biomarkers for disease severity and treatment response in atopic dermatitis (AD) are needed. Biopsies cause scarring and tape stripping represents an alternative minimally invasive method for stratum corneum sampling. In this study, we examined the gene expression of cytokines in skin biopsies and cytokines in stratum corneum tape strips collected from adults with AD. We collected punch biopsies and tape strips from healthy controls (<i>n</i> = 6) and subjects with AD (<i>n</i> = 12) at baseline and after 2 weeks of topical treatment with mometasone furoate 0.1% cream. We found that IFN-γ, IL-13, and IL-10 mRNA (biopsies) and IL-1β protein expression levels (tape strips) were significantly increased in lesional AD skin compared to healthy control skin. Treatment with topical corticosteroid led to a significant decrease in mRNA levels for IL-13 and IL-4R but no significant differences in cytokine protein levels measured in tape strips. Finally, we found no significant correlations between cytokine levels in tape strips and mRNA levels in skin biopsies.

scholarly journals Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy

2016 ◽  
Vol 170 (3) ◽  
pp. 187-193 ◽  
Author(s):  
Sjors A. Koppes ◽  
Richard Brans ◽  
Suzana Ljubojevic Hadzavdic ◽  
Monique H.W. Frings-Dresen ◽  
Thomas Rustemeyer ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Inflammatory Mediators ◽  
Topical Therapy ◽  
Tape Stripping

scholarly journals Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Maja-Lisa Clausen ◽  
H.-C. Slotved ◽  
Karen A. Krogfelt ◽  
Tove Agner
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Tape Stripping ◽  
Healthy Skin

scholarly journals Cytokine concentration across the stratum corneum in atopic dermatitis and healthy controls

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Maja-Lisa Clausen ◽  
S. Kezic ◽  
C. M. Olesen ◽  
T. Agner
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Skin Diseases ◽  
Sampling Strategy ◽  
Tape Stripping ◽  
Healthy Controls ◽  
Inflammatory Skin Diseases ◽  
Lesional Skin ◽  
Cytokine Milieu ◽  
Healthy Control

AbstractTape stripping is a promising technique for assessment of epidermal biomarkers in inflammatory skin diseases. However, to facilitate its implementation in the clinical practice, a thorough validation regarding sampling strategy is needed. Knowledge of biomarkers variation in concentration across stratum corneum is scarce. Therefore, this study aimed to assess the variability of cytokines across stratum corneum using tape stripping technique by consecutive application of 21 adhesive tapes (D-squame) to lesional and non-lesional skin from 15 patients with atopic dermatitis (AD) and 16 healthy controls. Concentration of cytokines (IL-1α, IL-1b, IL-5, IL-18, IFN-γ, CCL17, CCL22, CCL27, CXCL8, CXCL10, TNF-α, TSLP, VEGFA) was determined in five different depths, using multiplex immunoassay. Comparing tape 4 with tape 21, no cytokine changed significantly in concentration in AD lesional skin. In AD non-lesional skin a small decrease was found for CCL17, CXCL8 and CXCL10. For healthy controls, a decrease was found for IL-1a, IL-1b, VEGFA and an increase for IL-18. Differences were found between AD skin and healthy control skin. Concentration of cytokines was stable across stratum corneum, indicating that sampling of only one tape from the stratum corneum is reliable in reflecting the overall cytokine milieu. Differences between AD and healthy skin confirm robustness of tape stripping for measuring cytokine levels.

scholarly journals Measurement of trihydroxy-linoleic acids in stratum corneum by tape-stripping: Possible biomarker of barrier function in atopic dermatitis

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0210013 ◽  
Author(s):  
Takahito Chiba ◽  
Takeshi Nakahara ◽  
Futoshi Kohda ◽  
Toshio Ichiki ◽  
Motomu Manabe ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Barrier Function ◽  
Tape Stripping

scholarly journals Interleukin-8 Levels in the Stratum Corneum as a Biomarker for Monitoring Therapeutic Effect in Atopic Dermatitis Patients

2021 ◽  
pp. 1-15
Author(s):  
Susumu Murata ◽  
Sakae Kaneko ◽  
Eishin Morita
Keyword(s):  
Atopic Dermatitis ◽  
Topical Corticosteroid ◽  
Stratum Corneum ◽  
Corticosteroid Treatment ◽  
Skin Inflammation ◽  
Interleukin 8 ◽  
Tape Stripping ◽  
Therapeutic Effects ◽  
Local Skin ◽  
Before And After

<b><i>Introduction:</i></b> The stratum corneum contains several growth factors and cytokines that are synthesized in keratinocytes. We previously reported that the amount of interleukin-8 in the stratum corneum (scIL-8) is related to the severity of local skin inflammation in atopic dermatitis (AD). However, it is unknown whether scIL-8 levels reflect pharmacologic responses to a therapeutic intervention in AD patients. Therefore, in this study, we aimed to investigate whether the improvement of dermatitis in AD is correlated with scIL-8 levels before and after topical corticosteroid treatment. <b><i>Methods:</i></b> Stratum corneum samples were collected from 22 AD patients using the noninvasive tape-stripping method before treatment, 2 weeks after topical treatment, and 4–6 weeks after treatment. <b><i>Results:</i></b> scIL-8 levels on the forearm reduced significantly from 790 ± 348 pg/mg before treatment to 163 ± 68 pg/mg 2 weeks after treatment and 100 ± 37 pg/mg 4–6 weeks after corticosteroid treatment. scIL-8 levels on the abdomen also reduced significantly from 902 ± 391 to 142 ± 38 pg/mg at the end of study. The reduction in scIL-8 levels was associated with the improvement in local skin severity in AD. We also found that scIL-8 levels, along with blood biomarker levels (serum thymus and activation-regulated chemokine, lactate dehydrogenase, and %eosinophil), decreased significantly after the treatment. <b><i>Conclusion:</i></b> The scIL-8 concentration decreases with improvements in skin symptoms in AD patients after topical corticosteroid treatment; thus, it may be a suitable biomarker for monitoring therapeutic effects in AD patients.

The stratum corneum transcriptome in atopic dermatitis can be assessed by tape stripping

2020 ◽  
Author(s):  
Julie Sølberg ◽  
Stine B. Jacobsen ◽  
Jeppe D. Andersen ◽  
Thomas Litman ◽  
Nina H. Ulrich ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Stratum Corneum ◽  
Tape Stripping

Changes in corneocyte element concentrations occur in skin inner stratum corneum

1990 ◽  
Vol 48 (2) ◽  
pp. 146-147
Author(s):  
R. R. Warner
Keyword(s):  
Stratum Corneum ◽  
Human Skin ◽  
Element Concentration ◽  
Skin Barrier ◽  
Barrier Properties ◽  
Brick Wall ◽  
Inorganic Elements ◽  
Dry Skin ◽  
Skin Biopsies ◽  
Intercellular Lipid

Keratinocytes undergo maturation during their transit through the viable layers of skin, and then abruptly transform into flattened, anuclear corneocytes that constitute the cellular component of the skin barrier, the stratum corneum (SC). The SC is generally considered to be homogeneous in its structure and barrier properties, and is often shown schematically as a featureless brick wall, the “bricks” being the corneocytes, the “mortar” being intercellular lipid. Previously we showed the outer SC was not homogeneous in its composition, but contained steep gradients of the physiological inorganic elements Na, K and Cl, likely originating from sweat salts. Here we show the innermost corneocytes in human skin are also heterogeneous in composition, undergoing systematic changes in intracellular element concentration during transit into the interior of the SC.Human skin biopsies were taken from the lower leg of individuals with both “good” and “dry” skin and plunge-frozen in a stirred, cooled isopentane/propane mixture.

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