Site-Directed Spin Labeling for EPR Studies of Nucleic Acids

2016 ◽  
pp. 159-187 ◽  
Author(s):  
Sandip A. Shelke ◽  
Snorri Th. Sigurdsson
Keyword(s):  
Nucleic Acids ◽  
Spin Labeling ◽  
Site Directed Spin Labeling

scholarly journals Site-directed spin labeling measurements of nanometer distances in nucleic acids using a sequence-independent nitroxide probe

2006 ◽  
Vol 34 (17) ◽  
pp. 4722-4730 ◽  
Author(s):  
Qi Cai ◽  
Ana Karin Kusnetzow ◽  
Wayne L. Hubbell ◽  
Ian S. Haworth ◽  
Gian Paola C. Gacho ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Spin Labeling ◽  
Nitroxide Probe ◽  
Site Directed Spin Labeling

Site-directed spin labeling EPR spectroscopy in protein research

2013 ◽  
Vol 394 (10) ◽  
pp. 1281-1300 ◽  
Author(s):  
Johann P. Klare
Keyword(s):  
Nucleic Acids ◽  
Epr Spectroscopy ◽  
Protein Function ◽  
Conformational Dynamics ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Protein Research ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic ◽  
Natronomonas Pharaonis

Abstract Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy has emerged as an efficient tool to elucidate the structure and the conformational dynamics of proteins under conditions close to the native state. This review article summarizes the basics as well as the recent progress in SDSL and EPR methods, especially for investigations on protein structure, protein function, and interaction of proteins with other proteins or nucleic acids. Labeling techniques as well as EPR methods are introduced and exemplified with applications to systems that have been studied in the author’s laboratory in the past 15 years, headmost the sensory rhodopsin-transducer complex mediating the photophobic response of the halophilic archaeum Natronomonas pharaonis. Further examples underline the application of SDSL EPR spectroscopy to answer specific questions about the system under investigation, such as the nature and influence of interactions of proteins with other proteins or nucleic acids. Finally, it is discussed how SDSL EPR can be combined with other biophysical techniques to combine the strengths of the different methodologies.

Site-Directed Spin-Labeling of Nucleic Acids by Click Chemistry: Detection of Abasic Sites in Duplex DNA by EPR Spectroscopy

2010 ◽  
Vol 132 (30) ◽  
pp. 10424-10428 ◽  
Author(s):  
Ulla Jakobsen ◽  
Sandip A. Shelke ◽  
Stefan Vogel ◽  
Snorri Th. Sigurdsson
Keyword(s):  
Nucleic Acids ◽  
Click Chemistry ◽  
Epr Spectroscopy ◽  
Spin Labeling ◽  
Duplex Dna ◽  
Abasic Sites ◽  
Site Directed Spin Labeling

Noncovalent and Site-Directed Spin Labeling of Nucleic Acids

2010 ◽  
Vol 122 (43) ◽  
pp. 8156-8158 ◽  
Author(s):  
Sandip A. Shelke ◽  
Snorri Th. Sigurdsson
Keyword(s):  
Nucleic Acids ◽  
Spin Labeling ◽  
Site Directed Spin Labeling

Noncovalent and Site-Directed Spin Labeling of Nucleic Acids

2010 ◽  
Vol 49 (43) ◽  
pp. 7984-7986 ◽  
Author(s):  
Sandip A. Shelke ◽  
Snorri Th. Sigurdsson
Keyword(s):  
Nucleic Acids ◽  
Spin Labeling ◽  
Site Directed Spin Labeling

scholarly journals Correction: Braun, T., et al. Expanding the Genetic Code for Site-Directed Spin-Labeling. Int. J. Mol. Sci. 2019, 20, 373

2021 ◽  
Vol 22 (4) ◽  
pp. 1574
Author(s):  
Theresa Braun ◽  
Malte Drescher ◽  
Daniel Summerer
Keyword(s):  
Genetic Code ◽  
Spin Labeling ◽  
Site Directed Spin Labeling

The authors wish to make the following two corrections to this paper [...]

Nucleotide-dependent displacement and dynamics of the α-1 helix in kinesin revealed by site-directed spin labeling EPR

2014 ◽  
Vol 443 (3) ◽  
pp. 911-916 ◽  
Author(s):  
Satoshi Yasuda ◽  
Takanori Yanagi ◽  
Masafumi D. Yamada ◽  
Shoji Ueki ◽  
Shinsaku Maruta ◽  
...  
Keyword(s):  
Spin Labeling ◽  
Site Directed Spin Labeling

scholarly journals Membrane Docking Geometry of GRP1 PH Domain Bound to a Target Lipid Bilayer: An EPR Site-Directed Spin-Labeling and Relaxation Study

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33640 ◽  
Author(s):  
Huai-Chun Chen ◽  
Brian P. Ziemba ◽  
Kyle E. Landgraf ◽  
John A. Corbin ◽  
Joseph J. Falke
Keyword(s):  
Lipid Bilayer ◽  
Spin Labeling ◽  
Ph Domain ◽  
Relaxation Study ◽  
Site Directed Spin Labeling
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