DNA Barcoding of Mosquito Species

2018 ◽  
pp. 217-227
Author(s):  
Lalita Gupta ◽  
Sanjeev Kumar ◽  
Kuldeep Gupta
Keyword(s):  
Dna Barcoding ◽  
Mosquito Species

scholarly journals First Record of Mansonia dyari in the State of Morelos, Mexico, Based on Morphology and COI DNA Barcoding

2020 ◽  
Vol 36 (1) ◽  
pp. 33-36
Author(s):  
Luis F. Hernández-Guevara ◽  
Francisco J. Sánchez-Rámos ◽  
Rahuel J. Chan-Chable ◽  
Luis M. Hernández-Triana ◽  
Ma. Teresa Valdés-Perezgasga ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Mosquito Species ◽  
First Record ◽  
Morphological Characters ◽  
The State ◽  
Historical Records ◽  
Cytochrome C Oxidase I ◽  
Medical Importance

ABSTRACT Collections of mosquitoes were conducted for the surveillance of species of medical importance in the state of Morelos, Mexico, in June 2017. Species collected included Mansonia (Mansonia) dyari, which was identified using morphological characters and cytochrome c oxidase I DNA barcoding. Although 3 species of genus Mansonia have been previously reported in Mexico, this is the 1st confirmed record of Ma. dyari in Morelos State, where no Mansonia species had been recorded. Historical records of Ma. dyari and Ma. indubitans in Mexico were reviewed. Therefore, this record increases the number of mosquito species occurring in Morelos to 46. The specimens collected in this study were deposited in the Culicidae collection of the Universidad Autónoma Agraria Antonio Narro, Unidad Laguna.

scholarly journals DNA barcoding: complementing morphological identification of mosquito species in Singapore

2014 ◽  
Vol 7 (1) ◽  
Author(s):  
Abigail Chan ◽  
Lee-Pei Chiang ◽  
Hapuarachchige C Hapuarachchi ◽  
Cheong-Huat Tan ◽  
Sook-Cheng Pang ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Mosquito Species ◽  
Morphological Identification

scholarly journals Wing Morphometric and DNA Barcoding Analysis of Two Different Public Health Important Anopheles Mosquito Species

2021 ◽  
Vol 3 (23) ◽  
pp. 239-255
Author(s):  
Deepika Panda ◽  
Ipsita Biswal ◽  
Jaya Kishor Seth ◽  
Tapan Kumar Barik
Keyword(s):  
Public Health ◽  
Dna Barcoding ◽  
Mosquito Species ◽  
Anopheles Mosquito

scholarly journals New Records and Updated Checklist of Mosquito Species in Quintana Roo, Mexico, Using DNA-Barcoding

2020 ◽  
Vol 36 (4) ◽  
pp. 264-268
Author(s):  
Rahuel J. Chan-Chable ◽  
Arely Martínez-Arce ◽  
Aldo I. Ortega-Morales ◽  
Pedro C. Mis-Ávila
Keyword(s):  
Dna Barcoding ◽  
New Records ◽  
Mosquito Species ◽  
Quintana Roo ◽  
Vector Surveillance ◽  
Mexican State ◽  
Psorophora Columbiae ◽  
Molecular Characters

ABSTRACT Collections of mosquitoes were conducted as part of the entomological vector surveillance in Quintana Roo State, Mexico, during September 2015. Species collected included Anopheles gabaldoni, An. darlingi, Psorophora columbiae, Culex inflictus, Cx. trifidus, Cx. lactator, and Wyeomyia guatemala s.l. All the specimens were identified by morphological and molecular characters (DNA-barcoding). This is the 1st time these species are reported in the Mexican state of Quintana Roo. This research updates and increases the list of species of mosquitoes in Quintana Roo from 79 to 86.

Phylogenetics and molecular identification of theOchlerotatus communiscomplex (Diptera: Culicidae) using DNA barcoding and polymerase chain reaction-restriction fragment length polymorphism

2013 ◽  
Vol 146 (1) ◽  
pp. 26-35 ◽  
Author(s):  
Hooman H. Namin ◽  
Mahmood Iranpour ◽  
Barbara J. Sharanowski
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Dna Barcoding ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Mosquito Species ◽  
Morphological Characters ◽  
Oxidase Subunit ◽  
Restriction Fragment Length

AbstractTheOchlerotatus communis(De Geer, 1776) complex consists of four cryptic mosquito species in North America, including:O. communis,Ochlerotatus churchillensis(Ellis and Brust, 1973),Ochlerotatus nevadensis(Chapman and Barr, 1964), andOchlerotatus tahoensis(Dyar, 1916). Most of the morphological characters used for the identification of these species are quantitative and overlap across species. Here we evaluated the efficacy of DNA barcoding for identification of three members of thecommuniscomplex (O. nevadensisis not included in this study) and developed diagnostic restriction fragment length polymorphism (RFLP) patterns forO. communisandO. churchillensis. A phylogeny of 23OchlerotatusLynch Arribálzaga, 1891 species was inferred using mitochondrial cytochromecoxidase subunit I gene sequences. All species included in our analysis within theO. communiscomplex were delineated using cytochromecoxidase subunit I barcodes. However, this complex was recovered as paraphyletic with respect toOchlerotatus abserratus(Felt and Young, 1904) andOchlerotatus implicatus(Vockeroth, 1954), indicating the need for increased genetic and taxonomic sampling to infer the phylogenetic relationships of these taxa. The RFLP profile for multiple field specimens ofO. communiswas distinct from all RFLP patterns forO. churchillensis, and this method can be used as an efficient molecular method for the identification these species.

scholarly journals Identifying the Main Mosquito Species in China Based on DNA Barcoding

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47051 ◽  
Author(s):  
Gang Wang ◽  
Chunxiao Li ◽  
Xiaoxia Guo ◽  
Dan Xing ◽  
Yande Dong ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Mosquito Species

Identification of Belgian mosquito species (Diptera: Culicidae) by DNA barcoding

2014 ◽  
Vol 15 (2) ◽  
pp. 449-457 ◽  
Author(s):  
V. Versteirt ◽  
Z. T. Nagy ◽  
P. Roelants ◽  
L. Denis ◽  
F. C. Breman ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Mosquito Species

scholarly journals DNA barcoding of British mosquitoes (Diptera, Culicidae) to support species identification, discovery of cryptic genetic diversity and monitoring invasive species

ZooKeys ◽  
2019 ◽  
Vol 832 ◽  
pp. 57-76 ◽  
Author(s):  
Luis M. Hernández-Triana ◽  
Victor A. Brugman ◽  
Nadya I. Nikolova ◽  
Ignacio Ruiz-Arrondo ◽  
Elsa Barrero ◽  
...  
Keyword(s):  
Invasive Species ◽  
Dna Barcoding ◽  
Species Identification ◽  
Mosquito Species ◽  
Extraction Methods ◽  
Control Programs ◽  
Species Groups ◽  
Dna Extraction Methods ◽  
The Uk ◽  
Cryptic Genetic Diversity

Correct mosquito species identification is essential for mosquito and disease control programs. However, this is complicated by the difficulties in morphologically identifying some mosquito species. In this study, variation of a partial sequence of the cytochromecoxidase unit I (COI) gene was used for the molecular identification of British mosquito species and to facilitate the discovery of cryptic diversity, and monitoring invasive species. Three DNA extraction methods were compared to obtain DNA barcodes from adult specimens. In total, we analyzed 42 species belonging to the generaAedesMeigen, 1818 (21 species),AnophelesMeigen, 1818 (7 species),CoquillettidiaTheobald, 1904 (1 species),CulexLinnaeus, 1758 (6 species),CulisetaFelt, 1904 (7 species), andOrthopodomyiaTheobald, 1904 (1 species). Intraspecific genetic divergence ranged from 0% to 5.4%, while higher interspecific divergences were identified betweenAedesgeminusPeus, 1971/Culisetalitorea(Shute, 1928) (24.6%) andAe.geminus/An.plumbeusStephens, 1828 (22.5%). Taxonomic discrepancy was shown betweenAn.daciaeLinton, Nicolescu & Harbach, 2004 andAn.messeaeFalleroni, 1828 indicating the poor resolution of theCOIDNA barcoding region in separating these taxa. Other species such asAe.cantans(Meigen, 1818)/Ae.annulipes(Meigen, 1830) showed similar discrepancies indicating some limitation of this genetic marker to identify certain mosquito species. The combination of morphology and DNA barcoding is an effective approach for the identification of British mosquitoes, for invasive mosquitoes posing a threat to the UK, and for the detection of hidden diversity within species groups.

A DNA Barcoding Based Study to Identify Main Mosquito Species in Taiwan and its Difference from Those in Mainland China

2017 ◽  
Vol 20 (2) ◽  
Author(s):  
Bo Gao ◽  
Yiliang Fang ◽  
Jianqing Zhang ◽  
Rongquan Wu ◽  
Baohai Xu ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Mosquito Species ◽  
Mainland China
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