How Divergent Is the Cuatro Ciénegas Oasis? Genomic Studies of Microbial Populations and Niche Differentiation

2018 ◽  
pp. 57-71 ◽  
Author(s):  
Zulema Gomez-Lunar ◽  
Mirna Vázquez-Rosas-Landa ◽  
Gabriel Yaxal Ponce-Soto ◽  
Alejandra Moreno-Letelier ◽  
Gabriela Olmedo-Álvarez ◽  
...  
Keyword(s):  
Niche Differentiation ◽  
Microbial Populations ◽  
Genomic Studies ◽  
Cuatro Ciénegas

scholarly journals New Methods for Analysis of Spatial Distribution and Coaggregation of Microbial Populations in Complex Biofilms

2013 ◽  
Vol 79 (19) ◽  
pp. 5978-5987 ◽  
Author(s):  
Robert Almstrand ◽  
Holger Daims ◽  
Frank Persson ◽  
Fred Sörensson ◽  
Malte Hermansson
Keyword(s):  
Quantitative Methods ◽  
Niche Differentiation ◽  
Biofilm Reactor ◽  
Microbial Populations ◽  
Distribution Analysis ◽  
Content Type ◽  
New Methods ◽  
Nitrosomonas Oligotropha ◽  
Automated Tools ◽  
Sequential Fish

ABSTRACTIn biofilms, microbial activities form gradients of substrates and electron acceptors, creating a complex landscape of microhabitats, often resulting in structured localization of the microbial populations present. To understand the dynamic interplay between and within these populations, quantitative measurements and statistical analysis of their localization patterns within the biofilms are necessary, and adequate automated tools for such analyses are needed. We have designed and applied new methods for fluorescencein situhybridization (FISH) and digital image analysis of directionally dependent (anisotropic) multispecies biofilms. A sequential-FISH approach allowed multiple populations to be detected in a biofilm sample. This was combined with an automated tool for vertical-distribution analysis by generatingin silicobiofilm slices and the recently developed Inflate algorithm for coaggregation analysis of microbial populations in anisotropic biofilms. As a proof of principle, we show distinct stratification patterns of the ammonia oxidizersNitrosomonas oligotrophasubclusters I and II and the nitrite oxidizerNitrospirasublineage I in three different types of wastewater biofilms, suggesting niche differentiation between theN. oligotrophasubclusters, which could explain their coexistence in the same biofilms. Coaggregation analysis showed thatN. oligotrophasubcluster II aggregated closer toNitrospirathan didN. oligotrophasubcluster I in a pilot plant nitrifying trickling filter (NTF) and a moving-bed biofilm reactor (MBBR), but not in a full-scale NTF, indicating important ecophysiological differences between these phylogenetically closely related subclusters. By using high-resolution quantitative methods applicable to any multispecies biofilm in general, the ecological interactions of these complex ecosystems can be understood in more detail.

Water-sediment niche differentiation in ancient marine lineages of Exiguobacterium endemic to the Cuatro Cienegas Basin

2012 ◽  
Vol 14 (9) ◽  
pp. 2323-2333 ◽  
Author(s):  
Eria A. Rebollar ◽  
Morena Avitia ◽  
Luis E. Eguiarte ◽  
Andrea González-González ◽  
Lucy Mora ◽  
...  
Keyword(s):  
Niche Differentiation ◽  
Cuatro Ciénegas ◽  
Cuatro Ciénegas Basin

scholarly journals Ecological Specialization in a Spatially Structured Population of the Thermophilic Cyanobacterium Mastigocladus laminosus

2008 ◽  
Vol 75 (3) ◽  
pp. 729-734 ◽  
Author(s):  
Scott R. Miller ◽  
Carin Williams ◽  
Aaron L. Strong ◽  
Darla Carvey
Keyword(s):  
Population Sample ◽  
Natural Populations ◽  
Niche Differentiation ◽  
Laboratory Culture ◽  
Microbial Populations ◽  
Ecological Specialization ◽  
Selection Gradient ◽  
Mastigocladus Laminosus ◽  
Ecological Selection ◽  
Evolutionary Innovation

ABSTRACT Laboratory evolution experiments suggest the potential for microbial populations to contribute significant ecological variation to ecosystems, yet the functional importance of genetic diversity within natural populations of microorganisms is largely unknown. Here, we investigated the distribution of genetic and phenotypic variation for a population of the cyanobacterium Mastigocladus laminosus distributed along the temperature gradient of White Creek, Yellowstone NP. A total of 153 laboratory strains were directly isolated from five sites with mean annual temperatures ranging between 39 and 54�C. Genetic characterization at four nitrogen metabolism genes identified 15 closely related lineages in the population sample. These lineages were distributed nonrandomly along White Creek, but the observed geographic structure could not be explained by limited dispersal capabilities. Temperature performance experiments with six M. laminosus lineages that maximized their respective relative abundances at different positions along the gradient provided evidence for niche differentiation within the population. Niche differentiation included a tradeoff in performance at high and low temperatures, respectively. The physiological variation of these lineages in laboratory culture was generally well matched to the prevailing temperature conditions experienced by these organisms in situ. These results suggest that sympatric diversification along an ecological selection gradient can be a potent source of evolutionary innovation in microbial populations.

Modulation of the light field related to valve optical properties of raphid diatoms: implications for niche differentiation in the microphytobenthos

2018 ◽  
Vol 588 ◽  
pp. 29-42 ◽  
Author(s):  
JW Goessling ◽  
S Frankenbach ◽  
L Ribeiro ◽  
J Serôdio ◽  
M Kühl
Keyword(s):  
Optical Properties ◽  
Light Field ◽  
Niche Differentiation

Conservation Status and Sex Ratios of the Coahuila Box Turtle (Terrapene coahuila) in the Cuatro Ciénegas Valley of Coahuila, Mexico, 2011–2018

2020 ◽  
Vol 19 (1) ◽  
pp. 14
Author(s):  
Gamaliel Castañeda Gaytan ◽  
Ernesto Becerra-López ◽  
Sara Valenzuela-Ceballos ◽  
Miguel Borja-Jiménez ◽  
Bruno Rodríguez-López ◽  
...  
Keyword(s):  
Conservation Status ◽  
Sex Ratios ◽  
Box Turtle ◽  
Cuatro Ciénegas

The fate of formaldehyde in sugar manufacture and in products

2015 ◽  
pp. 692-696
Author(s):  
Remi Aubry ◽  
Laurence Gasnot
Keyword(s):  
Sugar Industry ◽  
Common Knowledge ◽  
Microbial Populations ◽  
Optimal Dosage ◽  
Consumer Health ◽  
Microbiological Activity ◽  
Specific Process ◽  
Beet Sugar ◽  
Series Of Experiments

A study was carried out in six beet sugar factories in France during the 2012/13 sugar campaign. The objective was to assess the optimal dosage of formaldehyde solutions at specific process stages and in different existing factory set-ups in order to obtain the desired effect on microbial populations, without interference with the quality of the products. In addition harmlessness regarding consumer health was to be demonstrated. A series of experiments was conducted resulting in new data allowing refreshment of common knowledge and references existing regarding the use of formaldehyde solutions in the sugar industry. The effectiveness and convenience for controlling microbiological activity in beet sugar manufacture was assessed. Formaldehyde reduces sugar losses and protects in-process products without harming their further use, such as for ethanol production.

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